Fitness for 5 genes in Escherichia coli BW25113

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500 ntttk and pyrE are separated by 36 nucleotidespyrE and rph are separated by 94 nucleotidesrph and yicC are separated by 126 nucleotidesyicC and dinD are separated by 220 nucleotides b3641: ttk - putative transcriptional regulator (VIMSS), at 3,812,517 to 3,813,113 ttk b3642: pyrE - orotate phosphoribosyltransferase (NCBI), at 3,813,150 to 3,813,791 pyrE b3643: rph - ribonuclease PH (NCBI), at 3,813,886 to 3,814,572 rph b3644: yicC - hypothetical protein (NCBI), at 3,814,699 to 3,815,562 yicC b3645: dinD - DNA-damage-inducible protein (VIMSS), at 3,815,783 to 3,816,607 dinD
Group Condition b3641 b3642 b3643 b3644 b3645
ttk pyrE rph yicC dinD
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin +0.0 -6.7 -0.5 -0.2 -0.1
msc 22hr_MSC_rep1_highinoculum -0.1 -6.8 +0.1 +0.1 -0.3
carbon source Ying_AminoAcid20 rep A; time point 2 +0.0 -7.9 +0.3 +0.6 -0.1
carbon source Ying_AminoAcid20 rep A; time point 3 -0.2 -7.9 +0.5 +0.9 -0.3
msc 22hr_mediaonly_rep1_lowinoculum +0.1 -7.1 -0.1 +0.3 -0.1
carbon source Ying_AminoAcid20 rep B; time point 3 +0.1 -7.6 +0.4 +0.8 -0.2
carbon source carbon source D-Mannose 1mM -0.0 -6.5 -0.6 +0.6 -0.1
carbon source Ying_AminoAcid20 rep B; time point 2 -0.0 -7.3 +0.3 +0.6 -0.1
carbon source Ying_OrganicAcid7 rep A; time point 3 +0.1 -7.7 +0.4 +1.0 -0.1
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.0 -7.4 +0.2 +0.9 -0.0
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 0.5x +0.0 -6.7 +0.7 +0.1 -0.1
carbon source Ying_sugar7 rep B; time point 2 +0.1 -7.9 +1.9 +0.5 -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 -0.1 -6.8 +0.5 +1.0 -0.2
carbon source Ying_sugar7 rep C; time point 2 +0.1 -7.9 +1.9 +0.5 -0.1
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.0 -6.3 +0.9 +0.3 -0.1
carbon source Ying_sugar7 rep A; time point 2 +0.0 -7.4 +1.9 +0.5 -0.1
stress RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM -0.3 -6.8 +1.6 +0.8 -0.1
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.1 -6.1 +0.4 +0.9 -0.1
carbon source Acetate (C) -0.1 -5.6 +0.3 +1.4 -0.1
carbon source Ying_sugar7 rep C; time point 3 -0.1 -7.6 +3.2 +0.7 -0.2
carbon source Ying_sugar7 rep B; time point 3 +0.1 -7.7 +3.2 +0.7 -0.2
stress RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM -0.4 -5.4 +1.7 +0.3 -0.1
carbon source Ying_sugar7 rep A; time point 3 +0.1 -7.7 +3.3 +0.6 -0.0
stress RCH2_defined_1percent_glucose with Chloride 100 mM -0.1 -5.6 +1.7 +0.4 -0.1
stress RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM -0.2 -4.9 +1.7 +0.4 -0.2
stress RCH2_defined_1percent_glucose with Chloride 200 mM -0.1 -5.0 +1.6 +0.6 -0.1
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.0 -6.1 +1.5 +2.1 -0.1
carbon source acetate (C) -0.6 -3.6 +0.3 +3.3 -0.3
carbon source L-Malic (C) -0.4 -3.5 +0.5 +2.9 -0.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.1 -4.0 +1.5 +2.3 -0.3
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