Fitness for 5 genes in Escherichia coli BW25113

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500 ntyibQ and yibD overlap by 14 nucleotidesyibD and tdh are separated by 238 nucleotidestdh and kbl are separated by 9 nucleotideskbl and htrL are separated by 274 nucleotides b3614: yibQ - orf, hypothetical protein (VIMSS), at 3,786,124 to 3,787,083 yibQ b3615: yibD - predicted glycosyl transferase (NCBI), at 3,787,070 to 3,788,104 yibD b3616: tdh - L-threonine 3-dehydrogenase (NCBI), at 3,788,343 to 3,789,368 tdh b3617: kbl - 2-amino-3-ketobutyrate coenzyme A ligase (NCBI), at 3,789,378 to 3,790,574 kbl b3618: htrL - involved in lipopolysaccharide biosynthesis (VIMSS), at 3,790,849 to 3,791,706 htrL
Group Condition b3614 b3615 b3616 b3617 b3618
yibQ yibD tdh kbl htrL
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 +0.3 -2.8 -2.9 +0.4
phage Ste41 -0.8 -0.6 -1.8 -1.6 -0.4
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.0 -0.2 -1.8 -2.5 -0.2
stress Benzalkonium Chloride 0.004 mg/ml +0.0 -0.2 -2.2 -2.6 +0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.0 -0.1 -1.6 -2.5 +0.2
motility outer cut, LB soft agar motility assay -0.0 +0.1 -1.1 -3.1 +0.2
phage RB68 -0.2 -0.4 -1.5 -1.7 -0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 +0.4 -2.4 -2.1 +0.4
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.1 -0.0 -2.4 -1.6 +0.1
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.1 -0.2 -1.7 -2.1 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.0 -0.1 -1.9 -2.0 +0.3
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.2 -0.2 -1.4 -2.4 +0.2
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 -0.1 -1.4 -2.1 +0.3
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.0 -0.2 -1.6 -1.7 -0.0
stress Cisplatin 0.1 mg/ml -0.3 -0.1 -1.1 -1.3 -0.6
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.0 -0.1 -1.9 -1.5 +0.2
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.0 -0.2 -1.7 -1.5 -0.0
phage Ste41 -0.3 -0.3 -1.8 -0.8 -0.1
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.1 +0.0 -2.0 -1.1 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.3 -0.9 -1.8 -0.4
stress Bacitracin 1 mg/ml -0.3 +0.1 -1.5 -1.6 +0.1
stress Cephalothin 0.01 mg/ml +0.0 +0.2 -1.2 -2.0 -0.1
phage RB68 -0.5 -0.3 -1.3 -0.9 -0.0
stress Bacitracin 0.5 mg/ml -0.1 +0.1 -1.7 -1.3 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.5 -0.1 -1.5 -1.2 -0.4
TN_18CS_warm37C_5dbl_rep1 +0.1 +0.1 -1.7 -1.0 +0.2
phage T7_phage 0.00015625 MOI TC3 -0.0 +0.3 -2.2 -0.5 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.2 -0.6 -1.6 -1.4 +0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.2 -0.1 -1.2 -1.4 +0.3
stress octanol 0.08 vol% +0.6 +0.1 -1.3 -1.0 +0.4
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