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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yibQ and yibD overlap by 14 nucleotides
yibD and tdh are separated by 238 nucleotides
tdh and kbl are separated by 9 nucleotides
kbl and htrL are separated by 274 nucleotides
b3614: yibQ - orf, hypothetical protein (VIMSS), at 3,786,124 to 3,787,083
yibQ
b3615: yibD - predicted glycosyl transferase (NCBI), at 3,787,070 to 3,788,104
yibD
b3616: tdh - L-threonine 3-dehydrogenase (NCBI), at 3,788,343 to 3,789,368
tdh
b3617: kbl - 2-amino-3-ketobutyrate coenzyme A ligase (NCBI), at 3,789,378 to 3,790,574
kbl
b3618: htrL - involved in lipopolysaccharide biosynthesis (VIMSS), at 3,790,849 to 3,791,706
htrL
Group
Condition
b3614
b3615
b3616
b3617
b3618
yibQ
yibD
tdh
kbl
htrL
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
+0.3
-2.8
-2.9
+0.4
phage
Ste41
-0.8
-0.6
-1.8
-1.6
-0.4
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.0
-0.2
-1.8
-2.5
-0.2
stress
Benzalkonium Chloride 0.004 mg/ml
+0.0
-0.2
-2.2
-2.6
+0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.0
-0.1
-1.6
-2.5
+0.2
motility
outer cut, LB soft agar motility assay
-0.0
+0.1
-1.1
-3.1
+0.2
phage
RB68
-0.2
-0.4
-1.5
-1.7
-0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
+0.4
-2.4
-2.1
+0.4
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.1
-0.0
-2.4
-1.6
+0.1
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.1
-0.2
-1.7
-2.1
+0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.0
-0.1
-1.9
-2.0
+0.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.2
-0.2
-1.4
-2.4
+0.2
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.2
-0.1
-1.4
-2.1
+0.3
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
-0.0
-0.2
-1.6
-1.7
-0.0
stress
Cisplatin 0.1 mg/ml
-0.3
-0.1
-1.1
-1.3
-0.6
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
-0.1
-1.9
-1.5
+0.2
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.0
-0.2
-1.7
-1.5
-0.0
phage
Ste41
-0.3
-0.3
-1.8
-0.8
-0.1
stress
1-ethyl-3-methylimidazolium chloride 40 mM
-0.1
+0.0
-2.0
-1.1
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
-0.3
-0.9
-1.8
-0.4
stress
Bacitracin 1 mg/ml
-0.3
+0.1
-1.5
-1.6
+0.1
stress
Cephalothin 0.01 mg/ml
+0.0
+0.2
-1.2
-2.0
-0.1
phage
RB68
-0.5
-0.3
-1.3
-0.9
-0.0
stress
Bacitracin 0.5 mg/ml
-0.1
+0.1
-1.7
-1.3
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.5
-0.1
-1.5
-1.2
-0.4
TN_18CS_warm37C_5dbl_rep1
+0.1
+0.1
-1.7
-1.0
+0.2
phage
T7_phage 0.00015625 MOI TC3
-0.0
+0.3
-2.2
-0.5
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+1.2
-0.6
-1.6
-1.4
+0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.2
-0.1
-1.2
-1.4
+0.3
stress
octanol 0.08 vol%
+0.6
+0.1
-1.3
-1.0
+0.4
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