Fitness for 5 genes in Escherichia coli BW25113

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500 ntyibT and yibL are separated by 284 nucleotidesyibL and lldP are separated by 371 nucleotideslldP and lldR overlap by 1 nucleotideslldR and lldD overlap by 4 nucleotides b4554: yibT - hypothetical protein (NCBI), at 3,774,194 to 3,774,403 yibT b3602: yibL - hypothetical protein (NCBI), at 3,774,688 to 3,775,050 yibL b3603: lldP - (R)-lactate / (S)-lactate / glycolate:H+ symporter LldP (from data), at 3,775,422 to 3,777,077 lldP b3604: lldR - DNA-binding transcriptional repressor (NCBI), at 3,777,077 to 3,777,853 lldR b3605: lldD - L-lactate dehydrogenase, FMN-linked (NCBI), at 3,777,850 to 3,779,040 lldD
Group Condition b4554 b3602 b3603 b3604 b3605
yibT yibL lldP lldR lldD
carbon source D,L-Lactate (C) -0.0 -0.1 -3.8 -3.1 -0.6
carbon source D,L-Lactate (C) +0.0 +0.1 -3.7 -2.4 -0.6
carbon source D-Lactate (C) +0.0 -0.1 -3.4 -1.6 +0.3
carbon source D-Lactate (C) +0.3 -0.3 -3.3 -1.4 +0.3
carbon source Glycolic Acid (C) +0.1 -0.4 -2.2 -0.4 +0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.0 +0.0 -1.2 -1.1 +0.1
carbon source L-Fucose (C) +0.2 -0.1 -0.6 -0.1 -1.1
carbon source Glycolic Acid (C) +0.2 -0.0 -2.1 -0.2 +0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 -0.4 -0.4 -0.7 +0.2
carbon source L-Fucose (C) +0.2 -0.2 -0.5 -0.2 -0.9
stress LB Aerobic with Irgasan 0.0000003125 mM -1.1 +0.1 -0.1 -0.4 +0.0
msc 22hr_MSC_rep2_lowinoculum +0.2 -0.1 -0.6 -0.6 -0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -1.0 +0.0 -0.6 +0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.0 +0.2 -0.7 -0.8 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.8 -0.5 +0.3 +0.1 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.3 -0.3 +0.4 -0.3 -0.9
stress octanol 0.08 vol% +0.8 -0.6 -0.4 -0.6 +0.1
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.5 -0.6 -0.1 +0.4 +0.3
phage T7_phage 0.0015625 MOI TC2 +0.8 -0.1 -0.5 -0.4 -0.0
carbon source L-Malic (C) -0.2 -0.2 -0.4 +0.3 +0.5
phage Ste41 -1.2 +0.6 -0.3 +0.2 +0.6
carbon source succinate (C) +0.0 -0.3 -0.4 +0.5 +0.5
carbon source carbon source D-Mannose 5mM +0.6 +0.2 +0.2 -0.4 -0.4
carbon source carbon source D-Glucose 0.25mM -0.5 +0.5 +0.0 -0.1 +0.5
control PBS_cage_1_mice _Feces_22 hr +1.2 -0.1 +0.1 -0.4 -0.1
control PBS_cage_1_mice _Feces_16 hr +1.2 -0.0 -0.0 -0.4 -0.1
nitrogen source L-Arginine (N) -0.2 -0.2 -0.1 +0.9 +0.4
carbon source Acetate (C) -0.3 +0.3 -0.1 +0.6 +0.5
stress Aluminum chloride 5 mM +0.3 -0.4 +0.3 +0.5 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.9 +1.0 -0.2 -0.0 +0.6
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