Fitness for 5 genes in Escherichia coli BW25113

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500 ntmtlA and mtlD are separated by 229 nucleotidesmtlD and mtlR overlap by 1 nucleotidesmtlR and yibT are separated by 11 nucleotidesyibT and yibL are separated by 284 nucleotides b3599: mtlA - Mannitol phosphotransferase system, IIA/IIB/IIC components (EC 2.7.1.197) (from data), at 3,770,304 to 3,772,217 mtlA b3600: mtlD - mannitol-1-phosphate 5-dehydrogenase (NCBI), at 3,772,447 to 3,773,595 mtlD b3601: mtlR - DNA-binding repressor (NCBI), at 3,773,595 to 3,774,182 mtlR b4554: yibT - hypothetical protein (NCBI), at 3,774,194 to 3,774,403 yibT b3602: yibL - hypothetical protein (NCBI), at 3,774,688 to 3,775,050 yibL
Group Condition b3599 b3600 b3601 b4554 b3602
mtlA mtlD mtlR yibT yibL
carbon source Carbon source D-Mannitol 5 mM -1.7 -5.8 +0.3 -0.0 +0.1
carbon source Carbon source D-Mannitol 10 mM -1.2 -5.6 +0.3 +0.2 -0.1
carbon source Carbon source D-Mannitol 5 mM -1.8 -4.7 +0.2 +0.0 +0.0
carbon source Carbon source D-Mannitol 2.5 mM -1.7 -4.1 +0.1 -0.1 -0.2
carbon source Carbon source D-Mannitol 10 mM -1.2 -5.0 +0.4 -0.0 -0.0
carbon source Carbon source D-Mannitol 2.5 mM -1.7 -4.2 +0.2 -0.0 -0.0
carbon source D-Sorbitol (C) -1.0 -4.1 -0.1 +0.2 -0.5
carbon source D-Sorbitol (C) -0.9 -4.3 -0.0 -0.1 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 -2.8 -0.7 -0.4 -0.4
stress 2,4-Dinitrophenol 2 mM -0.1 -0.6 -3.2 -0.1 -0.1
control PBS_cage_2_mouse_1_Cecum_40 hr -0.3 -1.6 -0.4 -0.8 -0.5
carbon source Glycolic Acid (C) +0.2 -0.3 -3.5 +0.2 -0.0
carbon source Glycolic Acid (C) +0.3 -0.2 -3.1 +0.1 -0.4
stress LB Aerobic with Spiramycin 0.0000625 mM -0.0 -2.8 -0.4 -0.4 +0.2
stress 2,4-Dinitrophenol 2 mM +0.1 -0.5 -2.8 -0.0 +0.0
stress LB Aerobic with Spiramycin 0.000125 mM +0.1 -2.4 -0.3 -0.5 -0.1
motility outer cut, LB soft agar motility assay +0.1 -0.5 -2.1 -0.8 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -2.5 -0.6 +0.1 -0.0
carbon source a-Ketoglutaric (C) +0.1 -0.4 -2.2 -0.1 -0.4
stress Benzalkonium Chloride 0.004 mg/ml +0.2 -0.8 -1.5 -0.8 +0.0
stress LB Aerobic with Spiramycin 0.0000625 mM +0.1 -3.0 +0.0 +0.2 -0.1
stress LB Aerobic with Spiramycin 0.000125 mM +0.0 -2.7 -0.1 -0.2 +0.3
carbon source Ying_sugar7 rep A; time point 3 +0.3 -2.0 -0.6 -0.2 -0.1
carbon source Ying_sugar7 rep C; time point 3 +0.3 -2.1 -0.2 -0.3 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.8 -3.2 -0.1 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.4 -2.3 -0.5 +0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +0.2 -2.2 +0.4 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +0.9 -2.4 -0.0 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.6 -2.2 +0.6 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.5 -1.3 +0.9 +1.0
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