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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
xylG and xylH overlap by 23 nucleotides
xylH and xylR are separated by 77 nucleotides
xylR and bax are separated by 195 nucleotides
bax and malS are separated by 319 nucleotides
b3567: xylG - fused D-xylose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,730,224 to 3,731,765
xylG
b3568: xylH - D-xylose transporter subunit (NCBI), at 3,731,743 to 3,732,924
xylH
b3569: xylR - DNA-binding transcriptional activator, xylose-binding (NCBI), at 3,733,002 to 3,734,180
xylR
b3570: bax - hypothetical protein (NCBI), at 3,734,376 to 3,735,200
bax
b3571: malS - periplasmic alpha-amylase precursor (NCBI), at 3,735,520 to 3,737,550
malS
Group
Condition
b3567
b3568
b3569
b3570
b3571
xylG
xylH
xylR
bax
malS
carbon source
D-Xylose (C)
-0.9
+0.1
-4.0
-0.0
-0.0
carbon source
D-Xylose (C)
-1.0
-0.0
-3.3
-0.2
-0.1
stress
Bacitracin 1 mg/ml
+0.0
-0.1
-0.2
-2.2
+0.1
stress
Trimethoprim 0.00125 mM
-0.1
-0.1
-0.1
-1.5
+0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
+0.5
+0.1
-1.8
+0.0
stress
Trimethoprim 0.0025 mM
-0.1
-0.3
+0.0
-1.1
-0.0
stress
Cephalothin 0.01 mg/ml
-0.0
-0.3
-0.3
-1.0
+0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.0
-0.2
+0.1
-1.1
+0.0
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.0
-0.3
-0.0
-0.9
-0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
-0.1
-0.2
-1.1
+0.4
phage
Δdbn_10
+0.0
-0.4
-0.0
-0.0
-0.7
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.0
-0.8
-0.3
+0.1
-0.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.2
-0.2
+0.0
-0.7
+0.2
carbon source
carbon source D-Glucose 0.25mM
+0.0
+0.1
-0.2
-0.4
-0.4
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
+0.1
-0.0
-0.2
-0.9
+0.2
carbon source
carbon source D-Glucose 0.25mM
-0.1
-0.9
+0.1
+0.3
-0.2
carbon source
carbon source D-Mannose 1mM
-0.1
-0.3
-0.3
+0.1
-0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
+0.3
-0.2
-0.9
+0.2
carbon source
D-Ribose (C)
-0.2
+0.4
-0.6
-0.3
+0.0
gfp_control
GFP_control
-0.0
+0.3
-0.3
-0.2
-0.2
carbon source
D-Ribose (C)
-0.1
+0.4
-0.5
-0.2
+0.0
stress
Bacitracin 0.5 mg/ml
+0.0
+0.1
-0.1
-0.7
+0.2
phage
Ste41
-0.1
-0.7
+0.2
+0.5
+0.1
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.2
-0.5
+0.3
+0.1
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.0
+0.6
+0.2
-0.6
-0.1
motility
outer cut, LB soft agar motility assay
+0.1
-0.2
+0.3
+0.5
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
-0.4
-0.0
+0.8
+0.3
stress
3-methyl-1-butanol 1.25 vol%
+0.1
+0.3
+0.5
-0.2
+0.0
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.1
+0.3
+0.2
+0.8
-0.1
motility
inner cut, LB soft agar motility assay
+0.2
+0.2
+0.2
+0.2
+0.5
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