Fitness for 5 genes in Escherichia coli BW25113

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500 ntmokA and insJ are separated by 47 nucleotidesinsJ and insK overlap by 4 nucleotidesinsK and sokA are separated by 26 nucleotidessokA and glyS are separated by 222 nucleotides b4647: mokA - no description, at 3,718,471 to 3,718,655 mokA b3557: insJ - IS150 protein InsA (NCBI), at 3,718,703 to 3,719,224 insJ b3558: insK - IS150 conserved protein InsB (NCBI), at 3,719,221 to 3,720,072 insK b4614: sokA - no description, at 3,720,099 to 3,720,128 sokA b3559: glyS - glycyl-tRNA synthetase subunit beta (NCBI), at 3,720,351 to 3,722,420 glyS
Group Condition b4647 b3557 b3558 b4614 b3559
mokA insJ insK sokA glyS
mzaabcde mzaABCDE -2.6 -0.4 -0.3 -0.9 N.D.
carbon source Casamino-acids -3.6 -0.3 +0.1 -0.2 N.D.
nophagecontrol No phage control -3.6 +0.1 -0.0 -0.4 N.D.
motility outer cut, LB soft agar motility assay -2.9 -0.2 +0.1 -0.8 N.D.
nophagecontrol No phage control -3.6 -0.0 -0.1 -0.0 N.D.
ppi ppI -2.9 -0.2 -0.1 -0.3 N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.8 -0.3 +0.2 -2.7 N.D.
dart Outgrowth from conjugation with pRH22 in LB+Gent50 -2.4 -0.2 +0.1 -1.0 N.D.
phage Utah_phage 15 MOI -3.6 +0.1 -0.0 +0.1 N.D.
carbon source Casamino-acids -3.4 -0.1 +0.0 +0.2 N.D.
drt_type_3 DRT_type_3 -2.8 +0.0 -0.1 -0.3 N.D.
carbon source carbon source D-Mannose 5mM -1.0 -0.1 -0.1 -2.1 N.D.
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan -2.9 +0.0 -0.0 -0.3 N.D.
gfp_control GFP_control -2.8 +0.0 -0.1 -0.3 N.D.
stress RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM -3.1 -0.0 +0.0 +0.0 N.D.
mixed community Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:1 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan -2.8 -0.2 +0.1 -0.0 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.2 +0.2 +0.1 -1.0 N.D.
phage Chi_phage 15 MOI -3.6 +0.3 +0.1 +0.3 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM +0.4 -0.4 -0.0 -2.3 N.D.
control PBS_cage_1_mice _Feces_16 hr +0.8 -0.2 -0.0 -2.5 N.D.
phage Mnm4 -2.2 -0.4 +0.1 +0.5 N.D.
msc 3hr_mediaonly_rep2_lowinoculum -2.8 +0.5 -0.0 +0.6 N.D.
msc 22hr_mediaonly_rep1_highinoculum -2.6 +0.1 +0.1 +0.6 N.D.
phage Mnm6 -2.4 -0.1 +0.0 +0.7 N.D.
msc 3hr_MSC_rep1_lowinoculum -2.7 +0.0 +0.0 +1.0 N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON +1.2 +0.1 -0.1 -2.9 N.D.
carbon source carbon source D-Glucose 5mM +2.6 -0.1 -0.3 -1.1 N.D.
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +2.6 -0.4 -0.0 -0.2 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +2.7 +0.2 +0.1 -0.5 N.D.
phage T7_phage 0.00015625 MOI TC4 +2.3 +0.3 +0.6 +0.2 N.D.
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