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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
bcsE and yhjT overlap by 4 nucleotides
yhjT and bcsG overlap by 4 nucleotides
bcsG and ldrD are separated by 86 nucleotides
ldrD and rdlD are separated by 48 nucleotides
b3536: bcsE - hypothetical protein (NCBI), at 3,694,481 to 3,696,052
bcsE
b3537: yhjT - orf, hypothetical protein (VIMSS), at 3,696,049 to 3,696,240
yhjT
b3538: bcsG - predicted inner membrane protein (NCBI), at 3,696,237 to 3,697,916
bcsG
b4453: ldrD - toxic polypeptide, small (NCBI), at 3,698,003 to 3,698,110
ldrD
b4454: rdlD - sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR-D repeat, with toxic peptide LdrD (RefSeq), at 3,698,159 to 3,698,224
rdlD
Group
Condition
b3536
b3537
b3538
b4453
b4454
bcsE
yhjT
bcsG
ldrD
rdlD
stress
octanol 0.08 vol%
-0.1
-0.3
-0.0
-3.1
-1.1
motility
outer cut, LB soft agar motility assay
-0.1
-0.0
-0.2
-3.5
-0.2
phage
Ste41
-0.4
-0.0
+0.2
-3.1
-0.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
+0.2
+0.1
-3.2
-0.3
stress
Cisplatin 0.1 mg/ml
-0.2
+0.1
+0.0
-3.1
+0.1
motility
inner cut, LB soft agar motility assay
-0.1
-0.3
-0.0
-3.3
+0.7
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.1
+0.3
+0.0
-2.5
-0.4
stress
toluene 2.5 vol%
-0.0
-0.3
-0.2
-1.6
-0.5
stress
3-methyl-1-butanol 1.25 vol%
+0.1
+0.1
-0.1
-2.7
+0.1
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
+0.2
-0.3
-0.1
-2.7
+0.3
gfp_control
GFP_control
-0.1
-1.0
-0.1
-0.0
-1.1
phage
R2_N4_T2_phage8
-0.2
-0.3
-0.3
-1.1
-0.5
carbon source
HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C)
+0.1
-0.1
+0.1
-2.0
-0.2
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.1
-0.3
+0.1
-2.3
+0.4
phage
Phage_N4_cage_3_mice _Feces_40 hr
+0.2
+0.1
-0.1
-1.8
+0.2
stress
Aluminum chloride 5 mM
-0.0
+0.4
+0.1
-1.6
-0.1
carbon source
acetate (C)
+0.1
+0.4
-0.0
-1.6
-0.1
carbon source
D-Glucose (C) MOPS
+0.0
+0.3
+0.0
-1.6
+0.2
motility
outer cut, LB soft agar motility assay
+0.1
-1.0
+0.2
-0.8
+0.6
carbon source
Dodecanol and Rhamnolipid (C)
+0.1
+0.6
-0.0
-1.5
+0.1
carbon source
carbon source D-Glucose 0.25mM
-0.1
+1.0
-0.0
-1.6
+0.3
phage
RB68
-0.1
+0.8
+0.0
-1.6
+0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
-1.1
+0.0
+1.3
-0.5
carbon source
carbon source D-Mannose 1mM
+0.2
+0.9
-0.0
-0.7
-0.5
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.1
-0.3
+0.3
-0.8
+1.1
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.2
+0.5
+0.2
-0.6
+0.8
phage
Δdbn_10
+0.2
+0.7
-0.0
+1.0
-0.4
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.2
+1.8
+0.0
-0.2
+0.1
phage
T7_phage 0.0015625 MOI TC2
-0.1
+0.6
-0.4
+1.2
+0.7
motility
inner cut, LB soft agar motility assay
+0.0
+1.0
+0.3
+1.2
+0.1
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