Fitness for 5 genes in Escherichia coli BW25113

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500 ntbcsE and yhjT overlap by 4 nucleotidesyhjT and bcsG overlap by 4 nucleotidesbcsG and ldrD are separated by 86 nucleotidesldrD and rdlD are separated by 48 nucleotides b3536: bcsE - hypothetical protein (NCBI), at 3,694,481 to 3,696,052 bcsE b3537: yhjT - orf, hypothetical protein (VIMSS), at 3,696,049 to 3,696,240 yhjT b3538: bcsG - predicted inner membrane protein (NCBI), at 3,696,237 to 3,697,916 bcsG b4453: ldrD - toxic polypeptide, small (NCBI), at 3,698,003 to 3,698,110 ldrD b4454: rdlD - sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR-D repeat, with toxic peptide LdrD (RefSeq), at 3,698,159 to 3,698,224 rdlD
Group Condition b3536 b3537 b3538 b4453 b4454
bcsE yhjT bcsG ldrD rdlD
stress octanol 0.08 vol% -0.1 -0.3 -0.0 -3.1 -1.1
motility outer cut, LB soft agar motility assay -0.1 -0.0 -0.2 -3.5 -0.2
phage Ste41 -0.4 -0.0 +0.2 -3.1 -0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 +0.2 +0.1 -3.2 -0.3
stress Cisplatin 0.1 mg/ml -0.2 +0.1 +0.0 -3.1 +0.1
motility inner cut, LB soft agar motility assay -0.1 -0.3 -0.0 -3.3 +0.7
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.1 +0.3 +0.0 -2.5 -0.4
stress toluene 2.5 vol% -0.0 -0.3 -0.2 -1.6 -0.5
stress 3-methyl-1-butanol 1.25 vol% +0.1 +0.1 -0.1 -2.7 +0.1
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.2 -0.3 -0.1 -2.7 +0.3
gfp_control GFP_control -0.1 -1.0 -0.1 -0.0 -1.1
phage R2_N4_T2_phage8 -0.2 -0.3 -0.3 -1.1 -0.5
carbon source HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) +0.1 -0.1 +0.1 -2.0 -0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.1 -0.3 +0.1 -2.3 +0.4
phage Phage_N4_cage_3_mice _Feces_40 hr +0.2 +0.1 -0.1 -1.8 +0.2
stress Aluminum chloride 5 mM -0.0 +0.4 +0.1 -1.6 -0.1
carbon source acetate (C) +0.1 +0.4 -0.0 -1.6 -0.1
carbon source D-Glucose (C) MOPS +0.0 +0.3 +0.0 -1.6 +0.2
motility outer cut, LB soft agar motility assay +0.1 -1.0 +0.2 -0.8 +0.6
carbon source Dodecanol and Rhamnolipid (C) +0.1 +0.6 -0.0 -1.5 +0.1
carbon source carbon source D-Glucose 0.25mM -0.1 +1.0 -0.0 -1.6 +0.3
phage RB68 -0.1 +0.8 +0.0 -1.6 +0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -1.1 +0.0 +1.3 -0.5
carbon source carbon source D-Mannose 1mM +0.2 +0.9 -0.0 -0.7 -0.5
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.1 -0.3 +0.3 -0.8 +1.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.2 +0.5 +0.2 -0.6 +0.8
phage Δdbn_10 +0.2 +0.7 -0.0 +1.0 -0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.2 +1.8 +0.0 -0.2 +0.1
phage T7_phage 0.0015625 MOI TC2 -0.1 +0.6 -0.4 +1.2 +0.7
motility inner cut, LB soft agar motility assay +0.0 +1.0 +0.3 +1.2 +0.1
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