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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
yhjJ and dctA are separated by 220 nucleotides
dctA and yhjK are separated by 182 nucleotides
yhjK and bcsC are separated by 81 nucleotides
bcsC and bcsZ overlap by 19 nucleotides
b3527: yhjJ - predicted zinc-dependent peptidase (NCBI), at 3,678,467 to 3,679,963
yhjJ
b3528: dctA - C4 dicarboxylate/orotate:H+ symporter (DctA)
(from data)
, at 3,680,184 to 3,681,470
dctA
b3529: yhjK - predicted diguanylate cyclase (RefSeq), at 3,681,653 to 3,683,641
yhjK
b3530: bcsC - cellulose synthase subunit (RefSeq), at 3,683,723 to 3,687,196
bcsC
b3531: bcsZ - endo-1,4-D-glucanase (NCBI), at 3,687,178 to 3,688,284
bcsZ
Group
Condition
b3527
b3528
b3529
b3530
b3531
yhjJ
dctA
yhjK
bcsC
bcsZ
carbon source
L-Malic (C)
-0.8
-4.2
-0.3
-0.1
-0.2
carbon source
L-Malic (C)
-0.7
-4.3
-0.3
-0.1
-0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.6
-0.3
-2.7
+0.1
-0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.5
-0.3
-2.6
+0.2
-0.1
carbon source
succinate (C)
-0.7
-3.8
-0.6
+0.0
-0.2
carbon source
succinate (C)
-0.8
-3.6
-0.5
-0.2
-0.2
stress
M9 with Methotrexate 1.98 mM 24 hr
-2.8
+0.1
-0.9
-0.1
-0.0
carbon source
a-Ketoglutaric (C)
-1.1
-0.9
-0.9
+0.1
-0.3
stress
Trimethoprim 0.00125 mM
-1.0
-0.1
-1.9
+0.0
-0.1
stress
Trimethoprim 0.0025 mM
-1.0
-0.1
-1.8
+0.0
-0.2
stress
M9 with Methotrexate 1.98 mM 24 hr
-1.7
-0.1
-0.4
-0.1
-0.3
stress
M9 with Methotrexate 1.98 mM 48 hr
-1.9
-0.0
-0.5
-0.0
-0.0
carbon source
a-Ketoglutaric (C)
-0.9
-0.6
-0.8
+0.1
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-2.9
+0.2
+0.3
-0.0
+0.1
nitrogen source
Putrescine (N)
-1.5
+0.0
-0.8
+0.1
-0.1
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
-1.6
+0.1
-0.7
-0.0
-0.1
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
-1.6
+0.1
-0.7
+0.0
-0.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.0
-0.4
-0.8
-0.1
+0.1
carbon source
Ying_OrganicAcid7 rep A; time point 3
+0.1
-1.9
-0.2
+0.0
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-0.2
-2.2
+0.3
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.3
-2.1
+0.2
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
-0.7
-1.8
+0.3
+0.3
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
-1.5
+0.4
-0.7
+0.0
+0.0
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
-1.5
+0.4
-0.6
+0.0
-0.0
phage
TP4
+0.1
-0.2
-1.6
+0.3
-0.3
phage
R2_N4_T1_phage10
-0.1
+0.4
-1.6
+0.2
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.1
-0.5
-1.3
+0.3
+0.3
stress
Benzalkonium Chloride 0.004 mg/ml
+0.8
+0.4
+0.1
+0.1
-1.1
carbon source
carbon source D-Glucose 20mM
+0.7
+0.1
+1.6
+0.2
+0.3
carbon source
carbon source D-Mannose 20mM
+0.3
+0.1
+2.2
+0.2
+0.2
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