Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhjJ and dctA are separated by 220 nucleotidesdctA and yhjK are separated by 182 nucleotidesyhjK and bcsC are separated by 81 nucleotidesbcsC and bcsZ overlap by 19 nucleotides b3527: yhjJ - predicted zinc-dependent peptidase (NCBI), at 3,678,467 to 3,679,963 yhjJ b3528: dctA - C4 dicarboxylate/orotate:H+ symporter (DctA) (from data), at 3,680,184 to 3,681,470 dctA b3529: yhjK - predicted diguanylate cyclase (RefSeq), at 3,681,653 to 3,683,641 yhjK b3530: bcsC - cellulose synthase subunit (RefSeq), at 3,683,723 to 3,687,196 bcsC b3531: bcsZ - endo-1,4-D-glucanase (NCBI), at 3,687,178 to 3,688,284 bcsZ
Group Condition b3527 b3528 b3529 b3530 b3531
yhjJ dctA yhjK bcsC bcsZ
carbon source L-Malic (C) -0.8 -4.2 -0.3 -0.1 -0.2
carbon source L-Malic (C) -0.7 -4.3 -0.3 -0.1 -0.4
stress LB Aerobic with Ramoplanin 0.000125 mM -2.6 -0.3 -2.7 +0.1 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM -2.5 -0.3 -2.6 +0.2 -0.1
carbon source succinate (C) -0.7 -3.8 -0.6 +0.0 -0.2
carbon source succinate (C) -0.8 -3.6 -0.5 -0.2 -0.2
stress M9 with Methotrexate 1.98 mM 24 hr -2.8 +0.1 -0.9 -0.1 -0.0
carbon source a-Ketoglutaric (C) -1.1 -0.9 -0.9 +0.1 -0.3
stress Trimethoprim 0.00125 mM -1.0 -0.1 -1.9 +0.0 -0.1
stress Trimethoprim 0.0025 mM -1.0 -0.1 -1.8 +0.0 -0.2
stress M9 with Methotrexate 1.98 mM 24 hr -1.7 -0.1 -0.4 -0.1 -0.3
stress M9 with Methotrexate 1.98 mM 48 hr -1.9 -0.0 -0.5 -0.0 -0.0
carbon source a-Ketoglutaric (C) -0.9 -0.6 -0.8 +0.1 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -2.9 +0.2 +0.3 -0.0 +0.1
nitrogen source Putrescine (N) -1.5 +0.0 -0.8 +0.1 -0.1
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -1.6 +0.1 -0.7 -0.0 -0.1
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -1.6 +0.1 -0.7 +0.0 -0.0
stress LB Aerobic with Irgasan 0.0000003125 mM -1.0 -0.4 -0.8 -0.1 +0.1
carbon source Ying_OrganicAcid7 rep A; time point 3 +0.1 -1.9 -0.2 +0.0 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -0.2 -2.2 +0.3 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.3 -2.1 +0.2 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -0.7 -1.8 +0.3 +0.3
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -1.5 +0.4 -0.7 +0.0 +0.0
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -1.5 +0.4 -0.6 +0.0 -0.0
phage TP4 +0.1 -0.2 -1.6 +0.3 -0.3
phage R2_N4_T1_phage10 -0.1 +0.4 -1.6 +0.2 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -0.5 -1.3 +0.3 +0.3
stress Benzalkonium Chloride 0.004 mg/ml +0.8 +0.4 +0.1 +0.1 -1.1
carbon source carbon source D-Glucose 20mM +0.7 +0.1 +1.6 +0.2 +0.3
carbon source carbon source D-Mannose 20mM +0.3 +0.1 +2.2 +0.2 +0.2
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