Fitness for 5 genes in Escherichia coli BW25113

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500 ntkdgK and yhjJ are separated by 95 nucleotidesyhjJ and dctA are separated by 220 nucleotidesdctA and yhjK are separated by 182 nucleotidesyhjK and bcsC are separated by 81 nucleotides b3526: kdgK - ketodeoxygluconokinase (VIMSS), at 3,677,442 to 3,678,371 kdgK b3527: yhjJ - predicted zinc-dependent peptidase (NCBI), at 3,678,467 to 3,679,963 yhjJ b3528: dctA - C4 dicarboxylate/orotate:H+ symporter (DctA) (from data), at 3,680,184 to 3,681,470 dctA b3529: yhjK - predicted diguanylate cyclase (RefSeq), at 3,681,653 to 3,683,641 yhjK b3530: bcsC - cellulose synthase subunit (RefSeq), at 3,683,723 to 3,687,196 bcsC
Group Condition b3526 b3527 b3528 b3529 b3530
kdgK yhjJ dctA yhjK bcsC
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -2.6 -0.3 -2.7 +0.1
carbon source L-Malic (C) -0.0 -0.8 -4.2 -0.3 -0.1
carbon source L-Malic (C) -0.2 -0.7 -4.3 -0.3 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -2.5 -0.3 -2.6 +0.2
carbon source succinate (C) -0.2 -0.7 -3.8 -0.6 +0.0
carbon source succinate (C) -0.0 -0.8 -3.6 -0.5 -0.2
carbon source D-Glucuronic Acid (C) -4.5 -0.0 +0.2 -0.2 -0.0
carbon source D-Glucuronic Acid (C) -4.6 +0.1 +0.3 -0.1 +0.0
carbon source D-Galacturonic Acid (C) -4.2 +0.1 +0.2 -0.1 -0.1
stress M9 with Methotrexate 1.98 mM 24 hr -0.0 -2.8 +0.1 -0.9 -0.1
carbon source D-Galacturonic Acid (C) -3.9 +0.1 +0.3 -0.1 +0.0
stress Trimethoprim 0.00125 mM -0.0 -1.0 -0.1 -1.9 +0.0
carbon source D-Galacturonic Acid (C) M9 -3.9 +0.0 +0.6 +0.3 -0.0
carbon source a-Ketoglutaric (C) -0.1 -1.1 -0.9 -0.9 +0.1
stress Trimethoprim 0.0025 mM +0.0 -1.0 -0.1 -1.8 +0.0
stress M9 with Methotrexate 1.98 mM 48 hr -0.0 -1.9 -0.0 -0.5 -0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -2.9 +0.2 +0.3 -0.0
nitrogen source Putrescine (N) -0.1 -1.5 +0.0 -0.8 +0.1
carbon source D-Galacturonic Acid (C) MOPS -3.9 +0.2 +0.9 +0.4 -0.0
carbon source a-Ketoglutaric (C) -0.0 -0.9 -0.6 -0.8 +0.1
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.0 -1.6 +0.1 -0.7 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.0 -0.7 -1.8 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.2 -0.3 -2.1 +0.2
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.2 +0.2 -1.9 -0.2 -0.0
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.1 -1.5 +0.4 -0.7 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.0 -0.2 -2.2 +0.3
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.0 -1.5 +0.4 -0.6 +0.0
carbon source carbon source D-Glucose 20mM -0.2 +0.8 +0.0 +1.4 -0.0
carbon source carbon source D-Glucose 20mM +0.2 +0.7 +0.1 +1.6 +0.2
carbon source carbon source D-Mannose 20mM +0.1 +0.3 +0.1 +2.2 +0.2
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