Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhjH and kdgK are separated by 231 nucleotideskdgK and yhjJ are separated by 95 nucleotidesyhjJ and dctA are separated by 220 nucleotidesdctA and yhjK are separated by 182 nucleotides b3525: yhjH - orf, hypothetical protein (VIMSS), at 3,676,443 to 3,677,210 yhjH b3526: kdgK - ketodeoxygluconokinase (VIMSS), at 3,677,442 to 3,678,371 kdgK b3527: yhjJ - predicted zinc-dependent peptidase (NCBI), at 3,678,467 to 3,679,963 yhjJ b3528: dctA - C4 dicarboxylate/orotate:H+ symporter (DctA) (from data), at 3,680,184 to 3,681,470 dctA b3529: yhjK - predicted diguanylate cyclase (RefSeq), at 3,681,653 to 3,683,641 yhjK
Group Condition b3525 b3526 b3527 b3528 b3529
yhjH kdgK yhjJ dctA yhjK
stress LB Aerobic with Ramoplanin 0.000125 mM -0.4 -0.1 -2.6 -0.3 -2.7
stress LB Aerobic with Ramoplanin 0.000125 mM -0.0 -0.2 -2.5 -0.3 -2.6
carbon source L-Malic (C) +0.0 -0.0 -0.8 -4.2 -0.3
carbon source L-Malic (C) +0.1 -0.2 -0.7 -4.3 -0.3
carbon source succinate (C) +0.1 -0.2 -0.7 -3.8 -0.6
carbon source succinate (C) -0.1 -0.0 -0.8 -3.6 -0.5
motility outer cut, LB soft agar motility assay -4.1 +0.2 -0.6 -0.1 -0.4
carbon source D-Glucuronic Acid (C) +0.1 -4.5 -0.0 +0.2 -0.2
carbon source D-Glucuronic Acid (C) +0.1 -4.6 +0.1 +0.3 -0.1
carbon source D-Galacturonic Acid (C) -0.2 -4.2 +0.1 +0.2 -0.1
stress M9 with Methotrexate 1.98 mM 24 hr -0.0 -0.0 -2.8 +0.1 -0.9
carbon source D-Galacturonic Acid (C) -0.0 -3.9 +0.1 +0.3 -0.1
stress Trimethoprim 0.00125 mM -0.0 -0.0 -1.0 -0.1 -1.9
stress Trimethoprim 0.0025 mM -0.1 +0.0 -1.0 -0.1 -1.8
carbon source a-Ketoglutaric (C) +0.1 -0.1 -1.1 -0.9 -0.9
carbon source D-Galacturonic Acid (C) M9 +0.3 -3.9 +0.0 +0.6 +0.3
nitrogen source Putrescine (N) -0.0 -0.1 -1.5 +0.0 -0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.0 +0.0 -0.7 -1.8
carbon source D-Galacturonic Acid (C) MOPS +0.1 -3.9 +0.2 +0.9 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.0 +0.2 -0.3 -2.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 +0.3 -0.0 -0.2 -2.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 +0.1 -2.9 +0.2 +0.3
carbon source Ying_OrganicAcid7 rep C; time point 3 -0.2 +0.2 +0.2 -1.9 -0.2
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -0.0 +0.1 -1.5 +0.4 -0.7
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.0 -0.0 -1.5 +0.4 -0.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.0 -0.2 -0.3 -0.6 +0.5
phage R2_N4_T2_phage10 +0.4 +0.5 -0.0 +0.1 -1.4
carbon source carbon source D-Glucose 20mM -0.1 -0.2 +0.8 +0.0 +1.4
carbon source carbon source D-Mannose 20mM +0.0 +0.1 +0.3 +0.1 +2.2
carbon source carbon source D-Glucose 20mM +0.1 +0.2 +0.7 +0.1 +1.6
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