Fitness for 5 genes in Escherichia coli BW25113

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500 ntuspA and yhiP are separated by 316 nucleotidesyhiP and yhiQ are separated by 48 nucleotidesyhiQ and prlC are separated by 7 nucleotidesprlC and yhiR are separated by 202 nucleotides b3495: uspA - universal stress global response regulator (NCBI), at 3,638,134 to 3,638,568 uspA b3496: yhiP - predicted transporter (NCBI), at 3,638,885 to 3,640,354 yhiP b3497: yhiQ - predicted SAM-dependent methyltransferase (NCBI), at 3,640,403 to 3,641,155 yhiQ b3498: prlC - oligopeptidase A (NCBI), at 3,641,163 to 3,643,205 prlC b3499: yhiR - predicted DNA (exogenous) processing protein (NCBI), at 3,643,408 to 3,644,250 yhiR
Group Condition b3495 b3496 b3497 b3498 b3499
uspA yhiP yhiQ prlC yhiR
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.3 -2.5 -1.0 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 -0.6 -1.8 -0.8 +0.2
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.0 -0.6 -1.4 -0.9 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -1.0 -1.1 -0.1 -0.1
ppi ppI +0.0 -0.6 -1.2 -0.3 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -0.2 -1.8 -0.8 +0.4
drt_type_3 DRT_type_3 +0.0 -0.5 -1.0 -0.2 -0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -0.6 -1.1 -0.1 +0.1
stress 2-nitrobenzenesulfonyl fluoride -0.1 +0.2 -0.2 -1.8 -0.1
gfp_control GFP_control +0.2 -0.4 -1.2 -0.2 -0.5
drt_type_3 DRT_type_3 +0.1 -0.5 -1.0 -0.3 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.8 -0.4 -0.7 -0.3 +0.2
mzaabcde mzaABCDE +0.1 -0.7 -0.8 -0.2 -0.3
mzaabcde mzaABCDE +0.1 -0.5 -0.8 -0.3 -0.4
phage Δdbn_10 -0.7 +0.3 -0.9 -0.0 -0.6
carbon source carbon source D-Glucose 0.25mM +0.2 -0.6 -0.0 -0.7 -0.6
drt_type_3 DRT_type_3 +0.3 -0.5 -0.9 -0.3 -0.2
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +0.4 -0.2 -1.6 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.4 -0.7 -0.9 -0.1
stress Aluminum chloride 5 mM +0.3 +1.4 +0.5 -3.1 -0.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.2 +0.5 -0.5 -0.9 +0.7
stress Cisplatin 0.1 mg/ml +0.4 +0.1 -0.2 -1.2 +0.5
stress 1-ethyl-3-methylimidazolium chloride 40 mM +0.3 +1.1 -0.5 -1.3 +0.2
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.6 +0.7 -0.4 -1.4 +0.4
m9 anaerobic no stress control M9 anaerobic no stress control +0.9 +0.2 -1.3 +0.1 +0.0
stress M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM +0.9 +0.3 -1.1 +0.1 -0.1
m9 anaerobic no stress control M9 anaerobic no stress control +0.9 +0.2 -1.1 +0.1 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +2.6 +0.1 -1.0 -0.5 -0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +2.2 +0.1 -0.5 -0.7 +0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 +0.5 +1.0 +0.4 +0.1
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