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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
uspA and yhiP are separated by 316 nucleotides
yhiP and yhiQ are separated by 48 nucleotides
yhiQ and prlC are separated by 7 nucleotides
prlC and yhiR are separated by 202 nucleotides
b3495: uspA - universal stress global response regulator (NCBI), at 3,638,134 to 3,638,568
uspA
b3496: yhiP - predicted transporter (NCBI), at 3,638,885 to 3,640,354
yhiP
b3497: yhiQ - predicted SAM-dependent methyltransferase (NCBI), at 3,640,403 to 3,641,155
yhiQ
b3498: prlC - oligopeptidase A (NCBI), at 3,641,163 to 3,643,205
prlC
b3499: yhiR - predicted DNA (exogenous) processing protein (NCBI), at 3,643,408 to 3,644,250
yhiR
Group
Condition
b3495
b3496
b3497
b3498
b3499
uspA
yhiP
yhiQ
prlC
yhiR
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.3
-2.5
-1.0
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
-0.6
-1.8
-0.8
+0.2
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.0
-0.6
-1.4
-0.9
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-1.0
-1.1
-0.1
-0.1
ppi
ppI
+0.0
-0.6
-1.2
-0.3
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-0.2
-1.8
-0.8
+0.4
drt_type_3
DRT_type_3
+0.0
-0.5
-1.0
-0.2
-0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.6
-1.1
-0.1
+0.1
stress
2-nitrobenzenesulfonyl fluoride
-0.1
+0.2
-0.2
-1.8
-0.1
gfp_control
GFP_control
+0.2
-0.4
-1.2
-0.2
-0.5
drt_type_3
DRT_type_3
+0.1
-0.5
-1.0
-0.3
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.8
-0.4
-0.7
-0.3
+0.2
mzaabcde
mzaABCDE
+0.1
-0.7
-0.8
-0.2
-0.3
mzaabcde
mzaABCDE
+0.1
-0.5
-0.8
-0.3
-0.4
phage
Δdbn_10
-0.7
+0.3
-0.9
-0.0
-0.6
carbon source
carbon source D-Glucose 0.25mM
+0.2
-0.6
-0.0
-0.7
-0.6
drt_type_3
DRT_type_3
+0.3
-0.5
-0.9
-0.3
-0.2
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
+0.4
-0.2
-1.6
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.4
-0.7
-0.9
-0.1
stress
Aluminum chloride 5 mM
+0.3
+1.4
+0.5
-3.1
-0.1
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.2
+0.5
-0.5
-0.9
+0.7
stress
Cisplatin 0.1 mg/ml
+0.4
+0.1
-0.2
-1.2
+0.5
stress
1-ethyl-3-methylimidazolium chloride 40 mM
+0.3
+1.1
-0.5
-1.3
+0.2
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.6
+0.7
-0.4
-1.4
+0.4
m9 anaerobic no stress control
M9 anaerobic no stress control
+0.9
+0.2
-1.3
+0.1
+0.0
stress
M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
+0.9
+0.3
-1.1
+0.1
-0.1
m9 anaerobic no stress control
M9 anaerobic no stress control
+0.9
+0.2
-1.1
+0.1
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.6
+0.1
-1.0
-0.5
-0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.2
+0.1
-0.5
-0.7
+0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
+0.5
+1.0
+0.4
+0.1
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