Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhiN and pitA are separated by 231 nucleotidespitA and yhiO are separated by 243 nucleotidesyhiO and uspA are separated by 390 nucleotidesuspA and yhiP are separated by 316 nucleotides b3492: yhiN - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 3,634,231 to 3,635,433 yhiN b3493: pitA - phosphate transporter, low-affinity (NCBI), at 3,635,665 to 3,637,164 pitA b3494: yhiO - universal stress protein UspB (NCBI), at 3,637,408 to 3,637,743 yhiO b3495: uspA - universal stress global response regulator (NCBI), at 3,638,134 to 3,638,568 uspA b3496: yhiP - predicted transporter (NCBI), at 3,638,885 to 3,640,354 yhiP
Group Condition b3492 b3493 b3494 b3495 b3496
yhiN pitA yhiO uspA yhiP
TN_15CS_warm37C_5dbl_rep1 +0.0 -4.8 -0.1 -0.0 -0.1
TN_15CS_warm37C_5dbl_rep2 -0.0 -4.3 -0.2 -0.0 -0.0
TN_15CS_warm37C_5dbl_rep3 -0.1 -4.2 +0.0 +0.0 -0.0
TN_15CS_15C_10dbl_rep1 +0.0 -4.1 +0.0 +0.0 +0.1
stress BHIS with Methotrexate 0.1 mM 24 hr -0.4 -2.2 -0.7 +0.6 -0.4
stress BHIS with Methotrexate 0.06 mM 48 hr -0.4 -2.1 -0.8 +0.6 -0.4
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.5 -2.0 -0.8 +0.6 -0.5
stress BHIS with Methotrexate 0.06 mM 24 hr -0.4 -2.2 -0.8 +0.7 -0.4
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -0.6 -2.0 -0.6 +0.6 -0.5
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -0.5 -2.0 -1.1 +0.6 +0.1
carbon source succinate (C) +0.4 -2.6 +0.6 -0.7 +0.3
carbon source L-Malic (C) +0.6 -2.4 +0.3 -0.6 +0.4
carbon source a-Ketoglutaric (C) +0.2 -2.1 +1.9 -1.0 +0.0
carbon source a-Ketoglutaric (C) +0.3 -2.0 +2.0 -0.7 +0.1
stress Trimethoprim 0.00125 mM +0.1 +3.8 -0.2 -0.3 -0.6
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.1 +3.8 +0.2 +0.0 -0.3
stress Trimethoprim 0.005 mM +0.0 +4.1 +0.0 -0.1 -0.1
stress Trimethoprim 0.0025 mM +0.1 +4.6 -0.2 -0.1 -0.4
stress LB Anaerobic with Trimethoprim 0.0000003125 mM -0.0 +3.9 -0.1 -0.0 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +4.6 +0.3 -0.0 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +4.0 +0.1 +0.2 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.0 +5.0 +0.1 +0.3 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +4.1 +0.7 +0.1 -0.1
stress LB Aerobic with Novobiocin 0.0000625 mM +0.1 +4.0 +1.0 -0.0 +0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.0 +5.6 -0.2 +0.1 -0.4
stress Cisplatin 0.1 mg/ml +0.1 +4.0 +0.7 +0.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 +4.3 +0.8 +0.3 -0.3
phage T7_phage 0.0015625 MOI TC2 -0.1 +5.9 -0.6 +0.3 -0.0
stress LB Aerobic with Novobiocin 0.0000625 mM +0.1 +4.9 +0.9 -0.1 -0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +4.2 +1.1 +0.1 +0.4
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