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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhiN and pitA are separated by 231 nucleotides
pitA and yhiO are separated by 243 nucleotides
yhiO and uspA are separated by 390 nucleotides
uspA and yhiP are separated by 316 nucleotides
b3492: yhiN - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 3,634,231 to 3,635,433
yhiN
b3493: pitA - phosphate transporter, low-affinity (NCBI), at 3,635,665 to 3,637,164
pitA
b3494: yhiO - universal stress protein UspB (NCBI), at 3,637,408 to 3,637,743
yhiO
b3495: uspA - universal stress global response regulator (NCBI), at 3,638,134 to 3,638,568
uspA
b3496: yhiP - predicted transporter (NCBI), at 3,638,885 to 3,640,354
yhiP
Group
Condition
b3492
b3493
b3494
b3495
b3496
yhiN
pitA
yhiO
uspA
yhiP
TN_15CS_warm37C_5dbl_rep1
+0.0
-4.8
-0.1
-0.0
-0.1
TN_15CS_warm37C_5dbl_rep2
-0.0
-4.3
-0.2
-0.0
-0.0
TN_15CS_warm37C_5dbl_rep3
-0.1
-4.2
+0.0
+0.0
-0.0
TN_15CS_15C_10dbl_rep1
+0.0
-4.1
+0.0
+0.0
+0.1
stress
BHIS with Methotrexate 0.1 mM 24 hr
-0.4
-2.2
-0.7
+0.6
-0.4
stress
BHIS with Methotrexate 0.06 mM 48 hr
-0.4
-2.1
-0.8
+0.6
-0.4
no stress control
BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
-0.5
-2.0
-0.8
+0.6
-0.5
stress
BHIS with Methotrexate 0.06 mM 24 hr
-0.4
-2.2
-0.8
+0.7
-0.4
no stress control
BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
-0.6
-2.0
-0.6
+0.6
-0.5
no stress control anaerobic
M9 no stress control; Anaerobic 24 hr
-0.5
-2.0
-1.1
+0.6
+0.1
carbon source
succinate (C)
+0.4
-2.6
+0.6
-0.7
+0.3
carbon source
L-Malic (C)
+0.6
-2.4
+0.3
-0.6
+0.4
carbon source
a-Ketoglutaric (C)
+0.2
-2.1
+1.9
-1.0
+0.0
carbon source
a-Ketoglutaric (C)
+0.3
-2.0
+2.0
-0.7
+0.1
stress
Trimethoprim 0.00125 mM
+0.1
+3.8
-0.2
-0.3
-0.6
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.1
+3.8
+0.2
+0.0
-0.3
stress
Trimethoprim 0.005 mM
+0.0
+4.1
+0.0
-0.1
-0.1
stress
Trimethoprim 0.0025 mM
+0.1
+4.6
-0.2
-0.1
-0.4
stress
LB Anaerobic with Trimethoprim 0.0000003125 mM
-0.0
+3.9
-0.1
-0.0
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
+4.6
+0.3
-0.0
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
+4.0
+0.1
+0.2
+0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
+5.0
+0.1
+0.3
-0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+4.1
+0.7
+0.1
-0.1
stress
LB Aerobic with Novobiocin 0.0000625 mM
+0.1
+4.0
+1.0
-0.0
+0.1
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.0
+5.6
-0.2
+0.1
-0.4
stress
Cisplatin 0.1 mg/ml
+0.1
+4.0
+0.7
+0.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
+4.3
+0.8
+0.3
-0.3
phage
T7_phage 0.0015625 MOI TC2
-0.1
+5.9
-0.6
+0.3
-0.0
stress
LB Aerobic with Novobiocin 0.0000625 mM
+0.1
+4.9
+0.9
-0.1
-0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
+4.2
+1.1
+0.1
+0.4
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