Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhhJ and rbbA overlap by 1 nucleotidesrbbA and yhiI overlap by 4 nucleotidesyhiI and yhiJ are separated by 365 nucleotidesyhiJ and yhiL are separated by 261 nucleotides b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826 yhhJ b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561 rbbA b3487: yhiI - predicted HlyD family secretion protein (NCBI), at 3,627,558 to 3,628,625 yhiI b3488: yhiJ - hypothetical protein (NCBI), at 3,628,991 to 3,630,613 yhiJ b4660: yhiL - no description, at 3,630,875 to 3,632,481 yhiL
Group Condition b3485 b3486 b3487 b3488 b4660
yhhJ rbbA yhiI yhiJ yhiL
motility inner cut, LB soft agar motility assay -0.6 +0.0 -0.2 -0.2 -2.2
stress Benzalkonium Chloride 0.004 mg/ml -0.2 -0.2 +0.4 +0.1 -2.8
M9 Glucose 2% anaerobic -0.1 +0.0 -0.0 -0.2 -2.2
M9 Glucose 2%, 20 mM NO3- anaerobic -0.1 +0.0 -0.0 -0.1 -2.0
carbon source L-Malic (C) +0.1 -0.1 -0.1 -0.3 -1.7
carbon source succinate (C) +0.2 +0.0 -0.4 -0.2 -1.5
carbon source acetate (C) +0.1 -0.0 -0.2 -0.2 -1.6
motility inner cut, LB soft agar motility assay -0.2 +0.2 -0.1 -0.1 -1.4
motility outer cut, LB soft agar motility assay -0.1 -0.1 +0.4 -0.2 -1.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.1 -0.8 -0.3 +0.8
stress octanol 0.08 vol% -0.2 +0.2 +0.2 +0.2 -1.2
phage Ste41 -0.6 -0.4 -0.1 -0.4 +1.1
phage Ste41 +0.2 -0.1 -0.6 -0.1 +1.2
control PBS_cage_2_mouse_1_Cecum_40 hr -0.2 +0.3 -0.4 +0.2 +0.9
phage Δdbn_10 -0.5 -0.1 +0.2 +0.4 +1.0
control PBS_cage_1_mice _Feces_16 hr -0.3 -0.1 -0.1 +0.3 +1.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.4 +0.1 +0.5 -0.2 +1.1
control PBS_cage_1_mouse_1_Cecum_40 hr +0.3 -0.1 -0.4 +0.5 +1.0
phage T7_phage 0.0015625 MOI TC1 -0.0 +0.0 +0.1 -0.3 +1.6
phage T7_phage 0.00015625 MOI TC3 +0.5 -0.1 -0.2 +0.4 +1.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.0 -0.1 +0.2 -0.0 +1.6
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 +0.2 +0.3 -0.1 +1.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.1 -0.1 +0.2 +0.4 +1.4
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.1 -0.0 +0.5 +0.2 +1.2
phage N4_phage 0.09375 MOI TC1 +0.1 +0.1 -0.1 +0.2 +1.6
stress M9 with Methotrexate 1.98 mM 24 hr +0.1 -0.0 -0.1 +0.2 +1.8
phage T7_phage 0.0015625 MOI TC2 -0.3 -0.1 +0.1 +0.3 +2.0
phage T7_phage 0.00015625 MOI TC4 +0.0 +0.1 +0.3 -0.0 +2.1
phage N4_phage 0.009375 MOI TC2 +0.1 +0.3 +0.2 +0.1 +2.6
phage Papa dilution 10-1 cacl2 maltose -0.0 +0.4 +0.5 -0.1 +2.9
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