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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhhJ and rbbA overlap by 1 nucleotides
rbbA and yhiI overlap by 4 nucleotides
yhiI and yhiJ are separated by 365 nucleotides
yhiJ and yhiL are separated by 261 nucleotides
b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826
yhhJ
b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561
rbbA
b3487: yhiI - predicted HlyD family secretion protein (NCBI), at 3,627,558 to 3,628,625
yhiI
b3488: yhiJ - hypothetical protein (NCBI), at 3,628,991 to 3,630,613
yhiJ
b4660: yhiL - no description, at 3,630,875 to 3,632,481
yhiL
Group
Condition
b3485
b3486
b3487
b3488
b4660
yhhJ
rbbA
yhiI
yhiJ
yhiL
motility
inner cut, LB soft agar motility assay
-0.6
+0.0
-0.2
-0.2
-2.2
stress
Benzalkonium Chloride 0.004 mg/ml
-0.2
-0.2
+0.4
+0.1
-2.8
M9 Glucose 2% anaerobic
-0.1
+0.0
-0.0
-0.2
-2.2
M9 Glucose 2%, 20 mM NO3- anaerobic
-0.1
+0.0
-0.0
-0.1
-2.0
carbon source
L-Malic (C)
+0.1
-0.1
-0.1
-0.3
-1.7
carbon source
succinate (C)
+0.2
+0.0
-0.4
-0.2
-1.5
carbon source
acetate (C)
+0.1
-0.0
-0.2
-0.2
-1.6
motility
inner cut, LB soft agar motility assay
-0.2
+0.2
-0.1
-0.1
-1.4
motility
outer cut, LB soft agar motility assay
-0.1
-0.1
+0.4
-0.2
-1.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-0.1
-0.8
-0.3
+0.8
stress
octanol 0.08 vol%
-0.2
+0.2
+0.2
+0.2
-1.2
phage
Ste41
-0.6
-0.4
-0.1
-0.4
+1.1
phage
Ste41
+0.2
-0.1
-0.6
-0.1
+1.2
control
PBS_cage_2_mouse_1_Cecum_40 hr
-0.2
+0.3
-0.4
+0.2
+0.9
phage
Δdbn_10
-0.5
-0.1
+0.2
+0.4
+1.0
control
PBS_cage_1_mice _Feces_16 hr
-0.3
-0.1
-0.1
+0.3
+1.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.4
+0.1
+0.5
-0.2
+1.1
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.3
-0.1
-0.4
+0.5
+1.0
phage
T7_phage 0.0015625 MOI TC1
-0.0
+0.0
+0.1
-0.3
+1.6
phage
T7_phage 0.00015625 MOI TC3
+0.5
-0.1
-0.2
+0.4
+1.1
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
-0.1
+0.2
-0.0
+1.6
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.2
+0.2
+0.3
-0.1
+1.4
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.1
-0.1
+0.2
+0.4
+1.4
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.1
-0.0
+0.5
+0.2
+1.2
phage
N4_phage 0.09375 MOI TC1
+0.1
+0.1
-0.1
+0.2
+1.6
stress
M9 with Methotrexate 1.98 mM 24 hr
+0.1
-0.0
-0.1
+0.2
+1.8
phage
T7_phage 0.0015625 MOI TC2
-0.3
-0.1
+0.1
+0.3
+2.0
phage
T7_phage 0.00015625 MOI TC4
+0.0
+0.1
+0.3
-0.0
+2.1
phage
N4_phage 0.009375 MOI TC2
+0.1
+0.3
+0.2
+0.1
+2.6
phage
Papa dilution 10-1 cacl2 maltose
-0.0
+0.4
+0.5
-0.1
+2.9
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