Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhhP and yhhQ are separated by 220 nucleotidesyhhQ and b3472 are separated by 72 nucleotidesb3472 and yhhS are separated by 3 nucleotidesyhhS and yhhT are separated by 131 nucleotides b3470: yhhP - cell developmental protein SirA (NCBI), at 3,606,774 to 3,607,019 yhhP b3471: yhhQ - conserved inner membrane protein (NCBI), at 3,607,240 to 3,607,905 yhhQ b3472: b3472 - orf, hypothetical protein (VIMSS), at 3,607,978 to 3,608,535 b3472 b3473: yhhS - putative transport (VIMSS), at 3,608,539 to 3,609,756 yhhS b3474: yhhT - orf, hypothetical protein (VIMSS), at 3,609,888 to 3,610,937 yhhT
Group Condition b3470 b3471 b3472 b3473 b3474
yhhP yhhQ b3472 yhhS yhhT
stress Trimethoprim 0.00125 mM N.D. +0.0 -4.6 +0.0 -0.0
stress Aluminum chloride 5 mM N.D. -0.1 -4.1 +0.2 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.3 -4.3 -0.1 +0.7
stress copper (II) chloride 2 mM N.D. -0.1 -3.3 -0.0 +0.2
stress Trimethoprim 0.0025 mM N.D. -0.0 -3.1 -0.1 +0.0
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 N.D. -0.2 -3.2 +0.2 +0.0
stress 2,4-Dinitrophenol 2 mM N.D. -0.1 -2.9 -0.1 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. +0.4 -3.3 +0.1 +0.0
stress Benzalkonium Chloride 0.004 mg/ml N.D. +0.0 -3.5 -0.1 +0.8
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. -0.9 -1.2 -0.2 -0.3
TN_15CS_warm37C_5dbl_rep1 N.D. -0.2 -2.3 -0.1 +0.0
control Mixed culture control experiment N.D. -0.1 -2.2 -0.1 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.3 -3.1 -0.1 +0.4
phage Ste41 N.D. -0.3 -1.4 -0.4 -0.3
TN_15CS_warm37C_5dbl_rep3 N.D. -0.2 -2.3 -0.0 +0.0
TN_15CS_warm37C_5dbl_rep2 N.D. -0.3 -2.3 +0.1 +0.1
motility outer cut, LB soft agar motility assay N.D. -0.4 -2.3 +0.0 +0.3
stress 2-Furfuraldehyde 0.25 vol% N.D. +0.1 -2.4 -0.1 +0.1
stress 2,4-Dinitrophenol 2 mM N.D. +0.1 -2.4 -0.0 +0.1
stress copper (II) chloride 2 mM N.D. -0.1 -2.3 +0.2 +0.0
phage Ste41 N.D. -0.1 -2.2 -0.1 +0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. -0.8 -1.1 -0.1 -0.2
stress Bacitracin 1 mg/ml N.D. -0.2 -1.9 -0.3 +0.3
no phage control Control_TnI N.D. -0.2 -1.8 -0.0 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.2 -1.0 -0.4 -0.5
carbon source carbon source D-Mannose 5mM N.D. +0.1 +1.5 +0.1 +0.2
stress undecene 5 vol% N.D. +0.3 +1.5 -0.0 +0.2
carbon source carbon source D-Mannose 5mM N.D. +0.1 +1.7 +0.3 -0.0
carbon source carbon source D-Mannose 20mM N.D. +0.1 +2.5 +0.1 +0.1
carbon source carbon source D-Mannose 20mM N.D. -0.0 +2.4 +0.1 +0.4
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