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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhhL and yhhM are separated by 2 nucleotides
yhhM and yhhN are separated by 140 nucleotides
yhhN and zntA are separated by 73 nucleotides
zntA and yhhP are separated by 101 nucleotides
b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271
yhhL
b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633
yhhM
b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400
yhhN
b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672
zntA
b3470: yhhP - cell developmental protein SirA (NCBI), at 3,606,774 to 3,607,019
yhhP
Group
Condition
b3466
b3467
b3468
b3469
b3470
yhhL
yhhM
yhhN
zntA
yhhP
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.2
-1.2
-2.9
-2.3
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.7
-1.1
-2.8
-1.8
N.D.
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.7
-0.8
-2.6
-2.1
N.D.
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.9
-0.8
-2.3
-1.4
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-5.6
-0.0
+0.4
+0.1
N.D.
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.4
+0.3
-0.2
-4.2
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-4.5
-0.2
+0.7
-0.1
N.D.
carbon source
Glycolic Acid (C)
+0.4
+0.1
-0.2
-4.0
N.D.
carbon source
acetate (C)
-0.6
+0.1
-0.0
-3.0
N.D.
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.2
-0.3
-0.7
-2.4
N.D.
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-2.3
-0.1
+0.4
-1.4
N.D.
carbon source
acetate (C)
-0.5
+0.2
-0.0
-3.0
N.D.
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.5
-0.6
+0.3
-1.4
N.D.
carbon source
Glycolic Acid (C)
+0.3
+0.2
-0.2
-3.6
N.D.
nitrogen source
Cytidine (N)
-0.1
-0.2
-0.1
-2.8
N.D.
nitrogen source
Cytidine (N)
-0.4
-0.2
-0.1
-2.5
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.2
-0.2
+0.3
-0.0
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-1.1
-0.1
-0.2
-1.7
N.D.
nitrogen source
Putrescine (N)
-0.5
+0.2
+0.1
-2.7
N.D.
phage
Ste41
-1.4
-0.7
-0.2
-0.6
N.D.
stress
LB Anaerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.2
-0.2
-0.4
-2.1
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-4.3
+0.1
+0.5
+0.8
N.D.
carbon source
Acetate (C)
-0.9
+0.1
-0.1
-2.0
N.D.
carbon source
Acetate (C)
-0.6
-0.2
+0.1
-2.0
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.6
+0.2
+0.5
+0.1
N.D.
nitrogen source
Putrescine (N)
-0.1
+0.1
+0.1
-2.7
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.4
-0.2
-0.1
+0.4
N.D.
carbon source
D-Galacturonic Acid (C)
-0.6
+0.0
+0.4
-1.9
N.D.
stress
M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
-0.5
+0.2
+0.3
-2.0
N.D.
carbon source
L-Malic (C)
+0.9
+0.2
-0.1
-1.7
N.D.
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