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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhhF and yhhL overlap by 11 nucleotides
yhhL and yhhM are separated by 2 nucleotides
yhhM and yhhN are separated by 140 nucleotides
yhhN and zntA are separated by 73 nucleotides
b3465: yhhF - predicted methyltransferase (NCBI), at 3,602,416 to 3,603,012
yhhF
b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271
yhhL
b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633
yhhM
b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400
yhhN
b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672
zntA
Group
Condition
b3465
b3466
b3467
b3468
b3469
yhhF
yhhL
yhhM
yhhN
zntA
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-4.2
-5.6
-0.0
+0.4
+0.1
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.2
-1.2
-1.2
-2.9
-2.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-4.0
-4.5
-0.2
+0.7
-0.1
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.4
-0.7
-1.1
-2.8
-1.8
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-1.5
-0.7
-0.8
-2.6
-2.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-3.2
-1.5
-0.6
+0.3
-1.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.7
-3.6
+0.2
+0.5
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-3.4
-4.3
+0.1
+0.5
+0.8
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.9
-0.9
-0.8
-2.3
-1.4
carbon source
Glycolic Acid (C)
-2.5
+0.4
+0.1
-0.2
-4.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-2.6
-2.3
-0.1
+0.4
-1.4
carbon source
Glycolic Acid (C)
-2.4
+0.3
+0.2
-0.2
-3.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.9
-2.4
-0.2
-0.1
+0.4
carbon source
acetate (C)
-1.2
-0.6
+0.1
-0.0
-3.0
nitrogen source
Putrescine (N)
-1.6
-0.5
+0.2
+0.1
-2.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.4
-3.2
-0.2
+0.3
-0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.0
-0.4
+0.3
-0.2
-4.2
nitrogen source
Putrescine (N)
-1.8
-0.1
+0.1
+0.1
-2.7
carbon source
acetate (C)
-0.9
-0.5
+0.2
-0.0
-3.0
stress
LB Aerobic with Oxytetracycline 0.00000125 mM
-3.8
+0.3
-0.3
-0.1
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-1.0
-1.1
-0.1
-0.2
-1.7
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.5
-0.2
-0.3
-0.7
-2.4
stress
LB Aerobic with Doxycycline hyclate 0.00000625 mM
-3.4
-0.2
-0.2
+0.0
-0.3
stress
LB Aerobic with Oxytetracycline 0.0000003125 mM
-2.6
+0.0
-0.3
-0.2
-0.8
stress
Cisplatin 0.1 mg/ml
-1.3
-1.1
-0.5
+0.1
-0.8
stress
M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
-1.4
-0.5
+0.2
+0.3
-2.0
m9 anaerobic no stress control
M9 anaerobic no stress control
-1.5
-0.4
+0.1
+0.2
-1.8
stress
M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
-1.2
-0.2
+0.2
+0.4
-2.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
+2.4
+0.3
-0.1
-0.4
-1.0
stress
LB Aerobic with Novobiocin 0.0000625 mM
+1.6
+1.0
+0.2
+0.7
-0.6
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