Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhhF and yhhL overlap by 11 nucleotidesyhhL and yhhM are separated by 2 nucleotidesyhhM and yhhN are separated by 140 nucleotidesyhhN and zntA are separated by 73 nucleotides b3465: yhhF - predicted methyltransferase (NCBI), at 3,602,416 to 3,603,012 yhhF b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271 yhhL b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633 yhhM b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400 yhhN b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672 zntA
Group Condition b3465 b3466 b3467 b3468 b3469
yhhF yhhL yhhM yhhN zntA
plasmid transformation mariner vector transformation; liquid outgrowth assay -4.2 -5.6 -0.0 +0.4 +0.1
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.2 -1.2 -1.2 -2.9 -2.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -4.0 -4.5 -0.2 +0.7 -0.1
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.4 -0.7 -1.1 -2.8 -1.8
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -1.5 -0.7 -0.8 -2.6 -2.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -3.2 -1.5 -0.6 +0.3 -1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -3.7 -3.6 +0.2 +0.5 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -3.4 -4.3 +0.1 +0.5 +0.8
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.9 -0.9 -0.8 -2.3 -1.4
carbon source Glycolic Acid (C) -2.5 +0.4 +0.1 -0.2 -4.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -2.6 -2.3 -0.1 +0.4 -1.4
carbon source Glycolic Acid (C) -2.4 +0.3 +0.2 -0.2 -3.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -2.9 -2.4 -0.2 -0.1 +0.4
carbon source acetate (C) -1.2 -0.6 +0.1 -0.0 -3.0
nitrogen source Putrescine (N) -1.6 -0.5 +0.2 +0.1 -2.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.4 -3.2 -0.2 +0.3 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.0 -0.4 +0.3 -0.2 -4.2
nitrogen source Putrescine (N) -1.8 -0.1 +0.1 +0.1 -2.7
carbon source acetate (C) -0.9 -0.5 +0.2 -0.0 -3.0
stress LB Aerobic with Oxytetracycline 0.00000125 mM -3.8 +0.3 -0.3 -0.1 -0.3
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -1.0 -1.1 -0.1 -0.2 -1.7
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -0.5 -0.2 -0.3 -0.7 -2.4
stress LB Aerobic with Doxycycline hyclate 0.00000625 mM -3.4 -0.2 -0.2 +0.0 -0.3
stress LB Aerobic with Oxytetracycline 0.0000003125 mM -2.6 +0.0 -0.3 -0.2 -0.8
stress Cisplatin 0.1 mg/ml -1.3 -1.1 -0.5 +0.1 -0.8
stress M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM -1.4 -0.5 +0.2 +0.3 -2.0
m9 anaerobic no stress control M9 anaerobic no stress control -1.5 -0.4 +0.1 +0.2 -1.8
stress M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM -1.2 -0.2 +0.2 +0.4 -2.0
stress LB Aerobic with Irgasan 0.0000003125 mM +2.4 +0.3 -0.1 -0.4 -1.0
stress LB Aerobic with Novobiocin 0.0000625 mM +1.6 +1.0 +0.2 +0.7 -0.6
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