Fitness for 5 genes in Escherichia coli BW25113

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500 ntugpQ and ugpC overlap by 4 nucleotidesugpC and ugpE are separated by 1 nucleotidesugpE and ugpA overlap by 4 nucleotidesugpA and ugpB are separated by 97 nucleotides b3449: ugpQ - cytoplasmic glycerophosphodiester phosphodiesterase (NCBI), at 3,585,393 to 3,586,136 ugpQ b3450: ugpC - ATP-binding component of sn-glycerol 3-phosphate transport system (VIMSS), at 3,586,133 to 3,587,203 ugpC b3451: ugpE - glycerol-3-phosphate transporter subunit (NCBI), at 3,587,205 to 3,588,050 ugpE b3452: ugpA - glycerol-3-phosphate transporter subunit (NCBI), at 3,588,047 to 3,588,934 ugpA b3453: ugpB - glycerol-3-phosphate transporter subunit (NCBI), at 3,589,032 to 3,590,348 ugpB
Group Condition b3449 b3450 b3451 b3452 b3453
ugpQ ugpC ugpE ugpA ugpB
stress octanol 0.08 vol% +0.2 -0.1 -1.4 -0.4 -0.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.6 -0.1 -0.3 -0.5 -0.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.4 -0.3 -0.3 -0.4 -0.2
phage T7_phage 0.00015625 MOI TC3 +0.2 -0.0 +0.3 -1.1 -0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 -0.4 -0.1 -0.1 +0.3
motility outer cut, LB soft agar motility assay -0.2 -0.2 +0.4 -0.7 -0.0
phage T7_phage 0.00015625 MOI TC4 +0.5 -0.7 +0.0 +0.1 -0.7
carbon source carbon source D-Mannose 1mM -0.7 -0.2 -0.1 +0.2 +0.3
carbon source acetate (C) -0.3 +0.2 -0.4 -0.3 +0.3
carbon source carbon source D-Glucose 5mM +0.2 -0.1 -1.0 +0.1 +0.3
carbon source L-Malic (C) -0.3 +0.2 -0.5 -0.1 +0.2
phage Lambda_ci1857 +0.2 +0.2 -0.5 +0.1 -0.5
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.1 -0.2 -0.5 +0.2 +0.4
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.0 +0.2 +0.5 -0.4 -0.3
drt_type_3 DRT_type_3 +0.3 +0.1 -0.6 -0.1 +0.3
mzaabcde mzaABCDE +0.1 +0.3 -0.7 +0.0 +0.4
stress LB Aerobic with Irgasan 0.0000003125 mM +0.4 -0.1 -0.2 -0.3 +0.4
stress 3-methyl-1-butanol 1.25 vol% +0.7 +0.1 +0.1 -0.2 -0.6
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.3 -0.6 +0.3 +0.3 -0.1
nophagecontrol R1_N4_T1_phage0 -0.6 +0.3 +0.4 -0.2 +0.5
gfp_control GFP_control +0.1 +0.2 -0.4 +0.3 +0.3
sir2-hera SIR2-HerA +0.5 +0.2 -0.6 +0.1 +0.4
gfp_control GFP_control +0.0 +0.4 -0.4 +0.3 +0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.3 +0.1 -0.4 +0.2 +0.4
control PBS_cage_1_mice _Feces_16 hr -0.7 +0.3 +0.1 +0.8 +0.0
stress Cisplatin 0.1 mg/ml +0.7 +0.3 +0.1 -0.1 -0.2
motility outer cut, LB soft agar motility assay +0.6 +0.5 -0.3 +0.0 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 +0.5 +0.2 +0.3 +0.0
phage Papa dilution 10-1 cacl2 maltose +0.5 +0.6 +0.4 +0.3 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.1 -0.3 +0.4 +0.5 +0.2
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