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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
yhgN and gntU are separated by 56 nucleotides
gntU and gntK are separated by 3 nucleotides
gntK and gntR are separated by 138 nucleotides
gntR and yhhW are separated by 223 nucleotides
b3434: yhgN - predicted antibiotic transporter (NCBI), at 3,573,094 to 3,573,687
yhgN
b4476: gntU - gluconate transporter, low affinity GNT 1 system (NCBI), at 3,573,744 to 3,575,084
gntU
b3437: gntK - gluconokinase 2, thermoresistant (VIMSS), at 3,575,088 to 3,575,615
gntK
b3438: gntR - DNA-binding transcriptional repressor (NCBI), at 3,575,754 to 3,576,749
gntR
b3439: yhhW - hypothetical protein (NCBI), at 3,576,973 to 3,577,668
yhhW
Group
Condition
b3434
b4476
b3437
b3438
b3439
yhgN
gntU
gntK
gntR
yhhW
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.4
-0.1
-1.7
-1.2
-0.6
retron_ec_48_control
retron_Ec_48_control
-0.1
+0.0
-0.3
-2.2
-0.1
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.4
-0.0
-1.6
-0.7
+0.2
gfp_control
GFP_control
-0.1
-0.1
+0.1
-2.4
-0.1
carbon source
D-Gluconic Acid (C)
-0.2
-0.0
-1.6
-0.7
+0.0
carbon source
D-Gluconic Acid (C)
-0.1
+0.0
-1.5
-0.8
-0.1
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
+0.1
-0.0
-0.8
-1.8
+0.1
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
-0.0
-0.0
-0.8
-1.7
+0.1
gfp_control
GFP_control
+0.0
-0.2
-0.3
-1.7
-0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.2
-0.2
-1.3
-0.9
+0.1
stress
2,4-Dinitrophenol 2 mM
+0.1
-0.1
-0.9
-1.2
-0.3
stress
Nickel (II) chloride 1.5 mM
+0.2
+0.1
-0.3
-2.1
-0.1
motility
outer cut, LB soft agar motility assay
-0.6
+0.1
-0.5
-1.3
+0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.2
-0.6
-1.1
+0.2
phage
T7_phage 0.00015625 MOI TC4
-0.5
-0.4
-0.2
-0.8
-0.2
carbon source
carbon source D-Mannose 0.5mM
-0.6
-0.2
-1.1
-0.3
+0.0
retron_ec_48_control
retron_Ec_48_control
+0.1
-0.1
-0.3
-1.8
+0.1
fishing
LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector
-0.2
+0.1
-0.6
-1.3
+0.0
gfp_control
GFP_control
+0.1
-0.1
+0.0
-1.8
-0.3
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
+0.0
+0.1
-0.5
-1.5
-0.0
mzaabcde
mzaABCDE
+0.1
-0.2
+0.0
-1.8
+0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
+0.3
-0.2
-1.7
-0.0
mzaabcde
mzaABCDE
+0.3
+0.0
+0.2
-2.0
-0.0
stress
Nickel (II) chloride 1.5 mM
+0.3
-0.0
+0.1
-1.3
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
+0.1
-0.3
-1.6
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
+0.2
-0.5
-1.3
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
+0.4
-0.1
-1.7
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
+0.3
+0.9
-0.9
-0.1
stress
Benzalkonium Chloride 0.004 mg/ml
-0.3
+0.5
-0.1
-0.6
+0.7
carbon source
Dodecanol (C)
+0.2
+0.1
+0.5
+5.9
+0.1
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