Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhgN and gntU are separated by 56 nucleotidesgntU and gntK are separated by 3 nucleotidesgntK and gntR are separated by 138 nucleotidesgntR and yhhW are separated by 223 nucleotides b3434: yhgN - predicted antibiotic transporter (NCBI), at 3,573,094 to 3,573,687 yhgN b4476: gntU - gluconate transporter, low affinity GNT 1 system (NCBI), at 3,573,744 to 3,575,084 gntU b3437: gntK - gluconokinase 2, thermoresistant (VIMSS), at 3,575,088 to 3,575,615 gntK b3438: gntR - DNA-binding transcriptional repressor (NCBI), at 3,575,754 to 3,576,749 gntR b3439: yhhW - hypothetical protein (NCBI), at 3,576,973 to 3,577,668 yhhW
Group Condition b3434 b4476 b3437 b3438 b3439
yhgN gntU gntK gntR yhhW
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.4 -0.1 -1.7 -1.2 -0.6
retron_ec_48_control retron_Ec_48_control -0.1 +0.0 -0.3 -2.2 -0.1
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.4 -0.0 -1.6 -0.7 +0.2
gfp_control GFP_control -0.1 -0.1 +0.1 -2.4 -0.1
carbon source D-Gluconic Acid (C) -0.2 -0.0 -1.6 -0.7 +0.0
carbon source D-Gluconic Acid (C) -0.1 +0.0 -1.5 -0.8 -0.1
dart Outgrowth from conjugation with pRH23 in LB+Gent50 +0.1 -0.0 -0.8 -1.8 +0.1
dart Outgrowth from conjugation with pRH23 in LB+Gent50 -0.0 -0.0 -0.8 -1.7 +0.1
gfp_control GFP_control +0.0 -0.2 -0.3 -1.7 -0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 -0.2 -1.3 -0.9 +0.1
stress 2,4-Dinitrophenol 2 mM +0.1 -0.1 -0.9 -1.2 -0.3
stress Nickel (II) chloride 1.5 mM +0.2 +0.1 -0.3 -2.1 -0.1
motility outer cut, LB soft agar motility assay -0.6 +0.1 -0.5 -1.3 +0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -0.2 -0.6 -1.1 +0.2
phage T7_phage 0.00015625 MOI TC4 -0.5 -0.4 -0.2 -0.8 -0.2
carbon source carbon source D-Mannose 0.5mM -0.6 -0.2 -1.1 -0.3 +0.0
retron_ec_48_control retron_Ec_48_control +0.1 -0.1 -0.3 -1.8 +0.1
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector -0.2 +0.1 -0.6 -1.3 +0.0
gfp_control GFP_control +0.1 -0.1 +0.0 -1.8 -0.3
dart Outgrowth from conjugation with pRH22 in LB+Gent50 +0.0 +0.1 -0.5 -1.5 -0.0
mzaabcde mzaABCDE +0.1 -0.2 +0.0 -1.8 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.3 -0.2 -1.7 -0.0
mzaabcde mzaABCDE +0.3 +0.0 +0.2 -2.0 -0.0
stress Nickel (II) chloride 1.5 mM +0.3 -0.0 +0.1 -1.3 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.1 -0.3 -1.6 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.2 -0.5 -1.3 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.4 -0.1 -1.7 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.3 +0.9 -0.9 -0.1
stress Benzalkonium Chloride 0.004 mg/ml -0.3 +0.5 -0.1 -0.6 +0.7
carbon source Dodecanol (C) +0.2 +0.1 +0.5 +5.9 +0.1
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