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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
glgC and glgX are separated by 17 nucleotides
glgX and glgB overlap by 4 nucleotides
glgB and asd are separated by 272 nucleotides
asd and yhgN are separated by 192 nucleotides
b3430: glgC - glucose-1-phosphate adenylyltransferase (NCBI), at 3,566,056 to 3,567,351
glgC
b3431: glgX - glycogen debranching enzyme (NCBI), at 3,567,369 to 3,569,342
glgX
b3432: glgB - glycogen branching enzyme (NCBI), at 3,569,339 to 3,571,525
glgB
b3433: asd - aspartate-semialdehyde dehydrogenase (NCBI), at 3,571,798 to 3,572,901
asd
b3434: yhgN - predicted antibiotic transporter (NCBI), at 3,573,094 to 3,573,687
yhgN
Group
Condition
b3430
b3431
b3432
b3433
b3434
glgC
glgX
glgB
asd
yhgN
nitrogen source
Putrescine (N)
-0.3
+0.1
-1.6
N.D.
+0.0
nitrogen source
Putrescine (N)
-0.2
+0.3
-2.0
N.D.
+0.1
nitrogen source
D-Alanine (N)
-0.4
-0.3
-0.8
N.D.
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.1
-0.3
-1.1
N.D.
-0.2
phage
T7_phage 0.00015625 MOI TC4
-0.8
+0.0
-0.3
N.D.
-0.5
phage
Lambda_ci1857
-0.2
-0.7
-0.6
N.D.
+0.0
motility
outer cut, LB soft agar motility assay
-0.2
-0.1
-0.4
N.D.
-0.6
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.3
+0.0
-1.1
N.D.
-0.4
carbon source
Glycolic Acid (C)
+0.2
-0.1
-1.2
N.D.
+0.1
control
PBS_cage_1_mice _Feces_16 hr
-0.0
+0.1
-0.6
N.D.
-0.4
carbon source
D-Serine (C)
+0.1
+0.1
-1.0
N.D.
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-0.5
+0.3
N.D.
-0.0
stress
octanol 0.08 vol%
-0.3
+0.3
-0.6
N.D.
-0.1
stress
1-ethyl-3-methylimidazolium chloride 80 mM
-0.3
-0.2
+0.3
N.D.
-0.5
carbon source
D-Serine (C)
-0.1
+0.2
-0.9
N.D.
+0.1
carbon source
Glycolic Acid (C)
+0.2
-0.3
-0.9
N.D.
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
-0.4
+0.3
N.D.
-0.4
carbon source
Ying_OrganicAcid7 rep A; time point 3
-0.5
-0.3
+0.1
N.D.
+0.3
carbon source
Ying_OrganicAcid7 rep C; time point 3
-0.6
-0.1
+0.2
N.D.
+0.3
carbon source
carbon source D-Glucose 20mM
-0.3
+0.5
-0.4
N.D.
+0.2
nitrogen source
L-Arginine (N)
+0.3
-0.2
-0.3
N.D.
+0.3
carbon source
D-Glucosamine Hydrochloride (C)
+0.4
-0.3
+0.3
N.D.
-0.2
carbon source
carbon source D-Mannose 0.5mM
+0.4
+0.2
+0.3
N.D.
-0.6
stress
Benzalkonium Chloride 0.004 mg/ml
+0.0
+1.4
-0.7
N.D.
-0.3
carbon source
D-Glucosamine Hydrochloride (C)
+0.6
-0.1
+0.4
N.D.
-0.5
stress
3-methyl-1-butanol 1.25 vol%
+0.0
+0.3
-0.3
N.D.
+0.6
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.3
+0.2
+0.0
N.D.
+0.5
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
+0.6
+0.3
+0.3
N.D.
-0.1
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
+0.8
+0.5
+0.6
N.D.
-0.0
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
+0.9
+0.5
+0.6
N.D.
-0.1
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