Fitness for 5 genes in Escherichia coli BW25113

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500 ntrtcB and rtcR are separated by 188 nucleotidesrtcR and glpR overlap by 19 nucleotidesglpR and glpG are separated by 16 nucleotidesglpG and glpE are separated by 44 nucleotides b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101 rtcB b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888 rtcR b3423: glpR - DNA-binding transcriptional repressor (NCBI), at 3,557,870 to 3,558,628 glpR b3424: glpG - predicted intramembrane serine protease (NCBI), at 3,558,645 to 3,559,475 glpG b3425: glpE - thiosulfate sulfurtransferase (NCBI), at 3,559,520 to 3,559,846 glpE
Group Condition b3421 b3422 b3423 b3424 b3425
rtcB rtcR glpR glpG glpE
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.2 -3.9 -2.1 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.0 -3.8 -1.9 +1.6
stress Trimethoprim 0.00125 mM +0.0 +0.0 -3.7 -0.4 +0.4
nitrogen source Putrescine (N) -0.0 -0.1 -3.0 +0.2 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.1 -2.2 -2.0 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.1 -1.7 -2.1 +0.1
stress Trimethoprim 0.0025 mM +0.0 -0.2 -3.1 -0.4 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 +0.1 -3.2 -1.8 +1.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.2 -0.2 -1.5 -0.4 -0.6
carbon source D-Ribose (C) -0.1 +0.1 -1.6 -0.2 -0.8
carbon source D-Ribose (C) -0.0 +0.1 -2.1 -0.1 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -0.0 -1.8 -1.2 +0.2
nitrogen source Putrescine (N) +0.1 +0.1 -2.3 +0.2 -0.4
stress hexanol 0.31 vol% -0.0 -0.0 +0.5 -2.5 -0.3
carbon source Glycolic Acid (C) +0.2 +0.0 -2.2 +0.2 -0.5
stress Nickel (II) chloride 1 mM -0.1 -0.0 -1.7 +0.2 -0.5
carbon source succinate (C) -0.0 -0.1 -2.6 +0.4 +0.2
carbon source succinate (C) -0.0 -0.1 -2.1 +0.4 +0.0
stress hexanol 0.31 vol% +0.1 +0.2 +0.3 -1.6 -0.3
phage Lambda_ci1857 +0.4 -0.2 +0.5 -0.3 -1.6
phage T7_phage 0.0015625 MOI TC2 -0.6 -0.6 +0.9 +0.0 -0.4
stress 3-methyl-1-butanol 1.25 vol% +0.1 +0.2 +1.3 -1.4 -0.3
stress 2,4-Dinitrophenol 2 mM -0.1 +0.0 +1.5 -0.1 -1.0
stress 3-methyl-1-butanol 1.25 vol% -0.2 +0.0 +1.5 -1.0 +0.2
stress octanol 0.08 vol% +0.2 +0.3 +1.7 -0.2 -1.1
stress 2,4-Dinitrophenol 2 mM +0.0 +0.1 +1.7 -0.1 -0.8
stress Benzalkonium Chloride 0.004 mg/ml +0.0 -0.0 +2.0 +0.0 -0.6
phage Papa dilution 10-1 cacl2 maltose +0.6 +1.5 +0.8 +0.5 +0.3
carbon source Dodecanol and Rhamnolipid (C) +0.0 +0.0 +4.2 +0.1 -0.2
carbon source Dodecanol and Rhamnolipid (C) +0.1 -0.0 +4.3 -0.1 +0.0
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