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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rtcB and rtcR are separated by 188 nucleotides
rtcR and glpR overlap by 19 nucleotides
glpR and glpG are separated by 16 nucleotides
glpG and glpE are separated by 44 nucleotides
b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101
rtcB
b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888
rtcR
b3423: glpR - DNA-binding transcriptional repressor (NCBI), at 3,557,870 to 3,558,628
glpR
b3424: glpG - predicted intramembrane serine protease (NCBI), at 3,558,645 to 3,559,475
glpG
b3425: glpE - thiosulfate sulfurtransferase (NCBI), at 3,559,520 to 3,559,846
glpE
Group
Condition
b3421
b3422
b3423
b3424
b3425
rtcB
rtcR
glpR
glpG
glpE
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
-0.2
-3.9
-2.1
+0.7
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
-0.0
-3.8
-1.9
+1.6
stress
Trimethoprim 0.00125 mM
+0.0
+0.0
-3.7
-0.4
+0.4
nitrogen source
Putrescine (N)
-0.0
-0.1
-3.0
+0.2
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
+0.1
-2.2
-2.0
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.1
-1.7
-2.1
+0.1
stress
Trimethoprim 0.0025 mM
+0.0
-0.2
-3.1
-0.4
+0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
+0.1
-3.2
-1.8
+1.6
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.2
-0.2
-1.5
-0.4
-0.6
carbon source
D-Ribose (C)
-0.1
+0.1
-1.6
-0.2
-0.8
carbon source
D-Ribose (C)
-0.0
+0.1
-2.1
-0.1
-0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
-0.0
-1.8
-1.2
+0.2
nitrogen source
Putrescine (N)
+0.1
+0.1
-2.3
+0.2
-0.4
stress
hexanol 0.31 vol%
-0.0
-0.0
+0.5
-2.5
-0.3
carbon source
Glycolic Acid (C)
+0.2
+0.0
-2.2
+0.2
-0.5
stress
Nickel (II) chloride 1 mM
-0.1
-0.0
-1.7
+0.2
-0.5
carbon source
succinate (C)
-0.0
-0.1
-2.6
+0.4
+0.2
carbon source
succinate (C)
-0.0
-0.1
-2.1
+0.4
+0.0
stress
hexanol 0.31 vol%
+0.1
+0.2
+0.3
-1.6
-0.3
phage
Lambda_ci1857
+0.4
-0.2
+0.5
-0.3
-1.6
phage
T7_phage 0.0015625 MOI TC2
-0.6
-0.6
+0.9
+0.0
-0.4
stress
3-methyl-1-butanol 1.25 vol%
+0.1
+0.2
+1.3
-1.4
-0.3
stress
2,4-Dinitrophenol 2 mM
-0.1
+0.0
+1.5
-0.1
-1.0
stress
3-methyl-1-butanol 1.25 vol%
-0.2
+0.0
+1.5
-1.0
+0.2
stress
octanol 0.08 vol%
+0.2
+0.3
+1.7
-0.2
-1.1
stress
2,4-Dinitrophenol 2 mM
+0.0
+0.1
+1.7
-0.1
-0.8
stress
Benzalkonium Chloride 0.004 mg/ml
+0.0
-0.0
+2.0
+0.0
-0.6
phage
Papa dilution 10-1 cacl2 maltose
+0.6
+1.5
+0.8
+0.5
+0.3
carbon source
Dodecanol and Rhamnolipid (C)
+0.0
+0.0
+4.2
+0.1
-0.2
carbon source
Dodecanol and Rhamnolipid (C)
+0.1
-0.0
+4.3
-0.1
+0.0
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