Fitness for 5 genes in Escherichia coli BW25113

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500 ntyrfG and hslR are separated by 10 nucleotideshslR and yrfI are separated by 24 nucleotidesyrfI and yhgE are separated by 62 nucleotidesyhgE and pck are separated by 378 nucleotides b3399: yrfG - putative phosphatase (VIMSS), at 3,526,691 to 3,527,359 yrfG b3400: hslR - ribosome-associated heat shock protein Hsp15 (NCBI), at 3,527,370 to 3,527,771 hslR b3401: yrfI - orf, hypothetical protein (VIMSS), at 3,527,796 to 3,528,674 yrfI b3402: yhgE - predicted inner membrane protein (NCBI), at 3,528,737 to 3,530,461 yhgE b3403: pck - phosphoenolpyruvate carboxykinase (NCBI), at 3,530,840 to 3,532,462 pck
Group Condition b3399 b3400 b3401 b3402 b3403
yrfG hslR yrfI yhgE pck
carbon source succinate (C) -0.1 +0.1 -0.8 +0.0 -2.5
phage Δdbn_10 -0.0 -0.8 -1.2 -0.1 -0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.0 +0.4 -0.4 -2.9
carbon source succinate (C) -0.1 +0.1 -0.6 +0.1 -2.0
carbon source Glycolic Acid (C) -0.2 +0.5 -1.0 -0.0 -1.7
carbon source L-Malic (C) -0.0 +0.1 -0.9 +0.0 -1.7
carbon source Glycolic Acid (C) -0.1 +0.6 -1.1 +0.0 -1.7
carbon source L-Malic (C) -0.0 +0.1 -0.8 +0.1 -1.5
motility outer cut, LB soft agar motility assay -0.6 -0.4 -0.5 -0.2 -0.1
carbon source carbon source D-Mannose 5mM -0.2 -0.1 -0.9 -0.4 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.1 +0.6 -0.3 -2.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.3 +1.0 -0.3 -2.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 +0.1 +0.9 -0.3 -2.4
carbon source a-Ketoglutaric (C) +0.1 +0.1 -0.9 +0.2 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 +0.1 +0.9 -0.1 -1.9
carbon source a-Ketoglutaric (C) +0.1 +0.3 -0.8 +0.2 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.1 +1.0 +0.0 -2.2
phage Ste41 +0.5 +0.2 -0.2 -0.0 -0.6
plasmid transformation vcDART vector transformation; agar assay +0.2 +0.2 +0.6 -0.0 -0.8
stress Trimethoprim 0.0025 mM +0.4 +0.3 +0.6 +0.1 -1.0
stress Trimethoprim 0.00125 mM +0.3 +0.4 +0.7 -0.0 -0.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.3 +0.1 +1.1 -0.2 -0.0
plasmid transformation vcDART vector transformation; agar assay +0.3 +0.3 +0.7 +0.1 -0.5
stress LB Aerobic with Novobiocin 0.0000625 mM +0.2 +0.2 +0.8 -0.1 -0.2
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.3 +1.0 +0.1 -0.1 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.1 +0.3 +1.1 -0.1 +0.2
protonated/deuterated deuterated glucose in D2O +0.1 +0.4 +0.9 +0.1 +0.0
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +1.1 -0.1 +0.6 +0.2 -0.1
protonated/deuterated deuterated glucose in D2O +0.0 +0.5 +1.1 +0.1 -0.0
stress Benzalkonium Chloride 0.004 mg/ml +0.5 +0.2 +1.3 +0.1 -0.0
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