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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yrfG and hslR are separated by 10 nucleotides
hslR and yrfI are separated by 24 nucleotides
yrfI and yhgE are separated by 62 nucleotides
yhgE and pck are separated by 378 nucleotides
b3399: yrfG - putative phosphatase (VIMSS), at 3,526,691 to 3,527,359
yrfG
b3400: hslR - ribosome-associated heat shock protein Hsp15 (NCBI), at 3,527,370 to 3,527,771
hslR
b3401: yrfI - orf, hypothetical protein (VIMSS), at 3,527,796 to 3,528,674
yrfI
b3402: yhgE - predicted inner membrane protein (NCBI), at 3,528,737 to 3,530,461
yhgE
b3403: pck - phosphoenolpyruvate carboxykinase (NCBI), at 3,530,840 to 3,532,462
pck
Group
Condition
b3399
b3400
b3401
b3402
b3403
yrfG
hslR
yrfI
yhgE
pck
carbon source
succinate (C)
-0.1
+0.1
-0.8
+0.0
-2.5
phage
Δdbn_10
-0.0
-0.8
-1.2
-0.1
-0.8
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
+0.0
+0.4
-0.4
-2.9
carbon source
succinate (C)
-0.1
+0.1
-0.6
+0.1
-2.0
carbon source
Glycolic Acid (C)
-0.2
+0.5
-1.0
-0.0
-1.7
carbon source
L-Malic (C)
-0.0
+0.1
-0.9
+0.0
-1.7
carbon source
Glycolic Acid (C)
-0.1
+0.6
-1.1
+0.0
-1.7
carbon source
L-Malic (C)
-0.0
+0.1
-0.8
+0.1
-1.5
motility
outer cut, LB soft agar motility assay
-0.6
-0.4
-0.5
-0.2
-0.1
carbon source
carbon source D-Mannose 5mM
-0.2
-0.1
-0.9
-0.4
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.1
+0.6
-0.3
-2.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
+0.3
+1.0
-0.3
-2.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
+0.1
+0.9
-0.3
-2.4
carbon source
a-Ketoglutaric (C)
+0.1
+0.1
-0.9
+0.2
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
+0.1
+0.9
-0.1
-1.9
carbon source
a-Ketoglutaric (C)
+0.1
+0.3
-0.8
+0.2
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
+0.1
+1.0
+0.0
-2.2
phage
Ste41
+0.5
+0.2
-0.2
-0.0
-0.6
plasmid transformation
vcDART vector transformation; agar assay
+0.2
+0.2
+0.6
-0.0
-0.8
stress
Trimethoprim 0.0025 mM
+0.4
+0.3
+0.6
+0.1
-1.0
stress
Trimethoprim 0.00125 mM
+0.3
+0.4
+0.7
-0.0
-0.9
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.3
+0.1
+1.1
-0.2
-0.0
plasmid transformation
vcDART vector transformation; agar assay
+0.3
+0.3
+0.7
+0.1
-0.5
stress
LB Aerobic with Novobiocin 0.0000625 mM
+0.2
+0.2
+0.8
-0.1
-0.2
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.3
+1.0
+0.1
-0.1
-0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.1
+0.3
+1.1
-0.1
+0.2
protonated/deuterated
deuterated glucose in D2O
+0.1
+0.4
+0.9
+0.1
+0.0
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+1.1
-0.1
+0.6
+0.2
-0.1
protonated/deuterated
deuterated glucose in D2O
+0.0
+0.5
+1.1
+0.1
-0.0
stress
Benzalkonium Chloride 0.004 mg/ml
+0.5
+0.2
+1.3
+0.1
-0.0
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