Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhfZ and trpS are separated by 289 nucleotidestrpS and gph overlap by 8 nucleotidesgph and rpe overlap by 8 nucleotidesrpe and dam are separated by 17 nucleotides b3383: yhfZ - hypothetical protein (NCBI), at 3,509,461 to 3,510,366 yhfZ b3384: trpS - tryptophanyl-tRNA synthetase (NCBI), at 3,510,656 to 3,511,660 trpS b3385: gph - phosphoglycolate phosphatase (NCBI), at 3,511,653 to 3,512,411 gph b3386: rpe - ribulose-phosphate 3-epimerase (NCBI), at 3,512,404 to 3,513,081 rpe b3387: dam - DNA adenine methylase (NCBI), at 3,513,099 to 3,513,935 dam
Group Condition b3383 b3384 b3385 b3386 b3387
yhfZ trpS gph rpe dam
motility outer cut, LB soft agar motility assay -0.2 N.D. -0.3 -3.1 -5.1
retron_ec_48_control retron_Ec_48_control -0.2 N.D. +0.1 -0.1 -6.9
carbon source D-Ribose (C) +0.1 N.D. -0.7 -2.4 -3.9
motility inner cut, LB soft agar motility assay -0.1 N.D. -0.3 -4.2 -2.1
motility outer cut, LB soft agar motility assay +0.0 N.D. -0.2 -3.7 -2.7
carbon source D-Ribose (C) +0.1 N.D. -0.7 -2.3 -3.8
carbon source Glycolic Acid (C) -0.0 N.D. -0.5 -2.2 -3.8
retron_ec_48_control retron_Ec_48_control +0.1 N.D. +0.1 -0.1 -6.5
motility inner cut, LB soft agar motility assay -0.2 N.D. -0.3 -4.2 -1.5
carbon source Glycolic Acid (C) -0.1 N.D. -0.4 -1.9 -4.0
retron_ec_48_control retron_Ec_48_control -0.0 N.D. +0.1 -0.0 -6.4
mzaabcde mzaABCDE -0.1 N.D. -0.0 +0.1 -6.1
motility inner cut, LB soft agar motility assay +0.0 N.D. -0.5 -3.9 -1.6
carbon source a-Ketoglutaric (C) +0.2 N.D. -1.1 -1.9 -3.1
carbon source a-Ketoglutaric (C) +0.2 N.D. -1.1 -1.7 -3.3
mzaabcde mzaABCDE -0.1 N.D. +0.0 +0.1 -5.8
sulfur source M63 no sulfur + 5 mM sulfate +0.2 N.D. +0.1 -5.0 -0.9
sulfur source M63 no sulfur + 5 mM L-cys +0.2 N.D. -0.1 -5.0 -0.5
carbon source D-Trehalose (C) +0.1 N.D. -0.3 -2.4 -2.7
carbon source L-Malic (C) +0.0 N.D. -0.3 -2.1 -3.0
mzaabcde mzaABCDE -0.2 N.D. -0.1 +0.1 -5.3
sulfur source M63 no sulfur + 5 mM L-cys +0.0 N.D. -0.0 -4.8 -0.5
carbon source L-Malic (C) +0.2 N.D. -0.1 -2.2 -3.1
motility outer cut, LB soft agar motility assay -0.1 N.D. +0.3 -3.5 -1.8
carbon source D-Trehalose (C) +0.2 N.D. +0.0 -2.4 -2.7
carbon source acetate (C) +0.2 N.D. -0.7 -2.2 -2.2
stress Cisplatin 0.1 mg/ml -0.9 N.D. -0.4 +1.1 -3.8
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.7 N.D. +0.0 +0.9 +3.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.4 N.D. +0.2 +0.8 +3.4
stress Aluminum chloride 5 mM -0.1 N.D. +0.1 +4.2 +1.1
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