Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhfU and php are separated by 10 nucleotidesphp and yhfW overlap by 4 nucleotidesyhfW and yhfX overlap by 1 nucleotidesyhfX and yhfY are separated by 83 nucleotides b3378: yhfU - orf, hypothetical protein (VIMSS), at 3,505,370 to 3,505,723 yhfU b3379: php - predicted hydrolase (NCBI), at 3,505,734 to 3,506,612 php b3380: yhfW - predicted mutase (NCBI), at 3,506,609 to 3,507,835 yhfW b3381: yhfX - predicted amino acid racemase (NCBI), at 3,507,835 to 3,508,998 yhfX b3382: yhfY - orf, hypothetical protein (VIMSS), at 3,509,082 to 3,509,444 yhfY
Group Condition b3378 b3379 b3380 b3381 b3382
yhfU php yhfW yhfX yhfY
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.9 -1.6 -0.3 -0.9 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -0.6 -0.5 -0.9 -0.3
stress Cisplatin 0.1 mg/ml -0.2 -1.1 -0.7 -0.6 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.8 -0.6 -1.3 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.9 -0.2 -0.9 -0.4
motility outer cut, LB soft agar motility assay -0.4 -0.6 -0.3 -0.2 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -1.1 -0.2 -0.7 +0.2
stress Cisplatin 0.1 mg/ml -0.0 -0.7 -0.4 -1.0 +0.2
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.3 -0.6 -0.2 -0.6 -0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.1 -0.7 -0.6 -0.7 +0.1
stress Cisplatin 0.1 mg/ml +0.1 -0.9 -0.5 -0.7 +0.1
phage Ste41 -0.7 -1.0 -0.2 +0.1 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.2 -0.8 -0.5 -1.1 +0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.3 -0.5 -0.2 -0.4 -0.4
stress Spectinomycin 0.0125 mg/ml +0.1 -0.8 -0.3 -0.7 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.5 -0.0 -0.9 -0.3
phage Ste41 -0.9 -0.2 -0.0 +0.2 -0.7
stress Cisplatin 0.05 mg/ml -0.1 -0.4 -0.3 -0.8 +0.1
stress Cisplatin 0.1 mg/ml +0.3 -0.6 -0.2 -0.6 -0.3
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.3 -0.2 -0.4 -0.8 -0.2
stress Spectinomycin 0.00625 mg/ml +0.5 -0.7 -0.4 -0.5 -0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.5 -0.8 -0.0 -0.4 -0.3
phage T7_phage 0.00015625 MOI TC4 +0.6 -0.5 -0.1 -0.0 -0.5
stress LB Aerobic with Irgasan 0.0000003125 mM -0.3 +0.4 +0.2 +0.3 -1.0
phage T7_phage 0.00015625 MOI TC3 -0.3 -0.4 -0.1 -0.2 +0.8
stress toluene 2.5 vol% +0.2 +0.3 +0.2 +0.1 -1.0
control PBS_cage_2_mouse_1_Cecum_40 hr +0.3 -0.3 -0.3 -0.5 +0.8
carbon source carbon source D-Glucose 0.25mM +0.5 -0.7 -0.0 -0.3 +0.8
carbon source carbon source D-Glucose 0.25mM +0.7 +0.6 +0.0 -0.3 -0.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.8 -0.0 +0.2 +0.0 +0.8
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