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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fic and yhfG overlap by 11 nucleotides
yhfG and ppiA are separated by 104 nucleotides
ppiA and tsgA are separated by 270 nucleotides
tsgA and nirB are separated by 261 nucleotides
b3361: fic - stationary-phase protein, cell division (NCBI), at 3,488,883 to 3,489,485
fic
b3362: yhfG - hypothetical protein (NCBI), at 3,489,475 to 3,489,642
yhfG
b3363: ppiA - peptidyl-prolyl cis-trans isomerase A (rotamase A) (NCBI), at 3,489,747 to 3,490,319
ppiA
b3364: tsgA - hypothetical protein (NCBI), at 3,490,590 to 3,491,771
tsgA
b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576
nirB
Group
Condition
b3361
b3362
b3363
b3364
b3365
fic
yhfG
ppiA
tsgA
nirB
stress
octanol 0.08 vol%
-0.9
+0.4
-3.4
-0.4
-0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.7
-0.8
-0.8
-0.1
-0.9
stress
Benzalkonium Chloride 0.004 mg/ml
+0.0
+0.1
-3.1
+0.1
+0.2
stress
Cephalothin 0.01 mg/ml
-0.5
-0.1
-1.5
-0.1
-0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.7
-0.2
-1.2
-0.1
-0.0
stress
Trimethoprim 0.0025 mM
-0.1
-0.3
-1.4
+0.0
-0.1
stress
Chlorite 0.4 mM
-0.9
-0.0
-0.3
-0.2
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.3
-0.4
+0.1
-0.5
stress
Carbenicillin 0.008 mg/ml
-0.5
-0.2
-0.5
-0.1
-0.1
stress
LB Anaerobic with Mecillinam 0.0000078125 mM
-1.0
-0.3
+0.1
-0.1
-0.0
stress
Trimethoprim 0.00125 mM
+0.0
-0.4
-1.2
+0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.6
-0.9
-0.1
+0.7
-0.3
carbon source
carbon source D-Mannose 0.5mM
-0.8
-0.4
+0.2
-0.1
+0.2
phage
T7_phage 0.0015625 MOI TC2
-1.2
+0.3
-0.0
-0.1
+0.1
predation
6 hr rep1_HD100_10fold_dilution_grewON
-1.5
+0.1
-0.1
+0.1
+0.4
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-1.1
+0.2
+0.1
-0.1
+0.0
motility
outer cut, LB soft agar motility assay
-1.1
-0.2
+0.2
+0.2
+0.0
stress
LB Anaerobic with Irgasan 0.000000625 mM
-0.2
-0.4
-0.6
-0.1
+0.6
stress
LB Aerobic with Novobiocin 0.0000625 mM
-0.3
+0.3
-0.8
+0.2
+0.0
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.2
-0.2
-0.7
-0.1
+0.4
carbon source
carbon source D-Glucose 5mM
+0.2
-0.8
+0.3
+0.1
-0.2
phage
Δdbn_10
-0.8
+0.4
+0.1
+0.1
-0.1
drt_type_3
DRT_type_3
+0.4
+0.3
-0.2
-0.2
-0.4
stress
Chlorite 1.25 mM
-0.9
-1.3
-1.0
+0.5
+2.6
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.5
+0.2
-0.7
-0.1
-0.0
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.2
+0.2
-0.4
-0.1
+0.7
phage
T7_phage 0.00015625 MOI TC4
+0.3
-0.2
+0.5
+0.2
+0.3
gfp_control
GFP_control
+0.9
+0.4
-0.1
+0.0
-0.2
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.5
+0.1
-0.3
+0.1
+0.6
stress
3-methyl-1-butanol 1.25 vol%
+1.3
+0.0
+0.4
+0.3
+0.2
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