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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fkpA and slyX are separated by 220 nucleotides
slyX and slyD are separated by 48 nucleotides
slyD and yheV are separated by 94 nucleotides
yheV and kefB are separated by 9 nucleotides
b3347: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI), at 3,474,629 to 3,475,441
fkpA
b3348: slyX - hypothetical protein (NCBI), at 3,475,662 to 3,475,880
slyX
b3349: slyD - FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI), at 3,475,929 to 3,476,519
slyD
b4551: yheV - hypothetical protein (NCBI), at 3,476,614 to 3,476,814
yheV
b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629
kefB
Group
Condition
b3347
b3348
b3349
b4551
b3350
fkpA
slyX
slyD
yheV
kefB
phage
T7_phage 0.0015625 MOI TC2
+0.2
-0.3
-1.7
-4.6
+0.0
stress
acetate 70 mM
-0.2
-1.3
+0.0
-2.1
+0.0
phage
Δdbn_10
-0.1
-1.2
-0.1
-1.6
-0.2
stress
Bacitracin 1 mg/ml
+0.8
-0.6
-1.7
-1.6
+0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.3
+0.6
-0.4
-1.9
-0.0
stress
LB Anaerobic with Oxytetracycline 0.0000003125 mM
+0.0
-0.5
-0.3
-2.2
+0.1
phage
T7_phage 0.00015625 MOI TC4
-0.2
+0.6
-1.2
-2.1
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
-0.5
-0.9
-1.4
+0.3
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.1
+0.1
-0.9
-2.3
+0.9
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.6
+0.3
-2.1
-0.1
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.9
+0.3
+0.9
-1.9
-0.2
motility
outer cut, LB soft agar motility assay
-0.4
-1.7
-0.3
+0.7
+0.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.9
-1.4
+1.0
+0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+1.7
-0.9
-2.3
-0.3
+0.4
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.1
+0.4
-1.9
+0.3
+0.5
stress
Benzalkonium Chloride 0.004 mg/ml
-0.2
-0.4
+1.4
-1.7
+0.1
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.4
+0.8
-1.8
+0.3
+0.6
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+2.0
-0.6
-1.6
-0.3
+0.3
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.2
+0.8
-0.8
-1.0
+0.7
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.5
+1.1
-2.0
+0.8
+0.6
stress
Carbenicillin 0.008 mg/ml
+2.1
-1.1
-0.4
-0.3
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.1
+0.6
+1.7
-0.7
-0.1
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.2
-1.6
+0.3
+1.4
+0.3
stress
Cephalothin 0.01 mg/ml
+1.7
-0.8
-0.6
+0.1
+0.2
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+1.0
-0.7
-0.5
+0.9
+0.1
stress
3-methyl-1-butanol 1.25 vol%
+0.2
-1.2
+1.3
+1.1
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+1.0
+0.7
-0.9
+1.1
+0.2
carbon source
Glycolic Acid (C)
+0.0
+0.5
+2.3
-0.6
+0.2
carbon source
Glycolic Acid (C)
-0.0
+0.8
+2.3
-0.2
+0.2
carbon source
carbon source D-Mannose 5mM
-0.1
+1.3
+0.7
+1.3
+0.3
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