Fitness for 5 genes in Escherichia coli BW25113

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500 ntfkpA and slyX are separated by 220 nucleotidesslyX and slyD are separated by 48 nucleotidesslyD and yheV are separated by 94 nucleotidesyheV and kefB are separated by 9 nucleotides b3347: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI), at 3,474,629 to 3,475,441 fkpA b3348: slyX - hypothetical protein (NCBI), at 3,475,662 to 3,475,880 slyX b3349: slyD - FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI), at 3,475,929 to 3,476,519 slyD b4551: yheV - hypothetical protein (NCBI), at 3,476,614 to 3,476,814 yheV b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629 kefB
Group Condition b3347 b3348 b3349 b4551 b3350
fkpA slyX slyD yheV kefB
phage T7_phage 0.0015625 MOI TC2 +0.2 -0.3 -1.7 -4.6 +0.0
stress acetate 70 mM -0.2 -1.3 +0.0 -2.1 +0.0
phage Δdbn_10 -0.1 -1.2 -0.1 -1.6 -0.2
stress Bacitracin 1 mg/ml +0.8 -0.6 -1.7 -1.6 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.3 +0.6 -0.4 -1.9 -0.0
stress LB Anaerobic with Oxytetracycline 0.0000003125 mM +0.0 -0.5 -0.3 -2.2 +0.1
phage T7_phage 0.00015625 MOI TC4 -0.2 +0.6 -1.2 -2.1 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -0.5 -0.9 -1.4 +0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.1 +0.1 -0.9 -2.3 +0.9
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.6 +0.3 -2.1 -0.1 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 +0.3 +0.9 -1.9 -0.2
motility outer cut, LB soft agar motility assay -0.4 -1.7 -0.3 +0.7 +0.2
motility outer cut, LB soft agar motility assay -0.2 -0.9 -1.4 +1.0 +0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.7 -0.9 -2.3 -0.3 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.1 +0.4 -1.9 +0.3 +0.5
stress Benzalkonium Chloride 0.004 mg/ml -0.2 -0.4 +1.4 -1.7 +0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.4 +0.8 -1.8 +0.3 +0.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM +2.0 -0.6 -1.6 -0.3 +0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 +0.8 -0.8 -1.0 +0.7
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.5 +1.1 -2.0 +0.8 +0.6
stress Carbenicillin 0.008 mg/ml +2.1 -1.1 -0.4 -0.3 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.1 +0.6 +1.7 -0.7 -0.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.2 -1.6 +0.3 +1.4 +0.3
stress Cephalothin 0.01 mg/ml +1.7 -0.8 -0.6 +0.1 +0.2
stress LB Anaerobic with Cefoxitin 0.000003125 mM +1.0 -0.7 -0.5 +0.9 +0.1
stress 3-methyl-1-butanol 1.25 vol% +0.2 -1.2 +1.3 +1.1 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.0 +0.7 -0.9 +1.1 +0.2
carbon source Glycolic Acid (C) +0.0 +0.5 +2.3 -0.6 +0.2
carbon source Glycolic Acid (C) -0.0 +0.8 +2.3 -0.2 +0.2
carbon source carbon source D-Mannose 5mM -0.1 +1.3 +0.7 +1.3 +0.3
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