Fitness for 5 genes in Escherichia coli BW25113

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500 ntgspO and bfr are separated by 28 nucleotidesbfr and bfd are separated by 71 nucleotidesbfd and chiA are separated by 168 nucleotideschiA and tufA are separated by 291 nucleotides b3335: gspO - bifunctional prepilin leader peptidase/ methylase (NCBI), at 3,463,565 to 3,464,242 gspO b3336: bfr - bacterioferritin, iron storage and detoxification protein (NCBI), at 3,464,271 to 3,464,747 bfr b3337: bfd - bacterioferritin-associated ferredoxin (NCBI), at 3,464,819 to 3,465,013 bfd b3338: chiA - periplasmic endochitinase (NCBI), at 3,465,182 to 3,467,875 chiA b3339: tufA - protein chain elongation factor EF-Tu (duplicate of tufB) (NCBI), at 3,468,167 to 3,469,351 tufA
Group Condition b3335 b3336 b3337 b3338 b3339
gspO bfr bfd chiA tufA
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -0.7 -0.9 -0.2 N.D.
phage N4_phage 0.009375 MOI TC2 -0.2 -0.5 -0.7 -0.3 N.D.
carbon source carbon source D-Glucose 0.25mM -0.5 -0.7 -0.4 -0.0 N.D.
nophagecontrol R1_N4_T1_phage0 -0.4 -0.6 -0.3 -0.2 N.D.
phage R2_N4_T1_phage10 -0.4 -0.1 -0.7 -0.1 N.D.
carbon source Glycolic Acid (C) -0.0 -0.8 -0.4 -0.0 N.D.
carbon source carbon source D-Mannose 5mM -0.0 -0.5 -0.4 -0.2 N.D.
stress M9 with Methotrexate 0.06 mM 48 hr +0.0 -0.2 -0.9 -0.0 N.D.
stress M9 with Methotrexate 0.06 mM 24 hr -0.0 -0.3 -0.8 +0.0 N.D.
phage N4_phage 0.009375 MOI TC1 -0.2 -0.3 -0.4 -0.2 N.D.
carbon source acetate (C) -0.0 -0.5 -0.6 +0.0 N.D.
stress Cisplatin 0.1 mg/ml -0.1 -0.2 -0.9 +0.1 N.D.
no stress control M9 no stress control; Aerobic 48 hr -0.1 -0.3 -0.7 -0.0 N.D.
carbon source carbon source D-Mannose 5mM -0.2 -0.2 -0.5 -0.1 N.D.
carbon source acetate (C) -0.2 -0.4 -0.5 +0.0 N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.2 -0.8 +0.1 N.D.
stress 3-methyl-1-butanol 1.25 vol% +0.2 -0.2 -0.7 +0.1 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 -0.5 -0.3 +0.1 N.D.
carbon source D-Serine (C) +0.6 -0.0 -0.6 -0.1 N.D.
phage T7_phage 0.00015625 MOI TC4 +0.3 +0.7 +0.1 +0.0 N.D.
phage T7_phage 0.0015625 MOI TC2 -0.1 +0.4 +0.6 +0.3 N.D.
control PBS_cage_2_mouse_1_Cecum_40 hr -0.1 +0.6 +0.4 +0.2 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.2 +0.2 +0.6 +0.2 N.D.
phage Papa dilution 10-1 cacl2 maltose +0.1 +0.2 +0.9 -0.0 N.D.
mzaabcde mzaABCDE -0.0 +0.2 +0.8 +0.2 N.D.
phage T7_phage 0.0015625 MOI TC1 +0.3 +0.5 +0.2 +0.2 N.D.
phage Ste41 +0.4 +0.4 +1.1 -0.2 N.D.
phage RB68 +0.5 +0.5 +0.8 -0.0 N.D.
phage Ste41 +0.5 +0.6 +0.8 -0.2 N.D.
phage RB68 +0.6 +0.5 +0.8 +0.1 N.D.
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