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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
gspO and bfr are separated by 28 nucleotides
bfr and bfd are separated by 71 nucleotides
bfd and chiA are separated by 168 nucleotides
chiA and tufA are separated by 291 nucleotides
b3335: gspO - bifunctional prepilin leader peptidase/ methylase (NCBI), at 3,463,565 to 3,464,242
gspO
b3336: bfr - bacterioferritin, iron storage and detoxification protein (NCBI), at 3,464,271 to 3,464,747
bfr
b3337: bfd - bacterioferritin-associated ferredoxin (NCBI), at 3,464,819 to 3,465,013
bfd
b3338: chiA - periplasmic endochitinase (NCBI), at 3,465,182 to 3,467,875
chiA
b3339: tufA - protein chain elongation factor EF-Tu (duplicate of tufB) (NCBI), at 3,468,167 to 3,469,351
tufA
Group
Condition
b3335
b3336
b3337
b3338
b3339
gspO
bfr
bfd
chiA
tufA
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-0.7
-0.9
-0.2
N.D.
phage
N4_phage 0.009375 MOI TC2
-0.2
-0.5
-0.7
-0.3
N.D.
carbon source
carbon source D-Glucose 0.25mM
-0.5
-0.7
-0.4
-0.0
N.D.
nophagecontrol
R1_N4_T1_phage0
-0.4
-0.6
-0.3
-0.2
N.D.
phage
R2_N4_T1_phage10
-0.4
-0.1
-0.7
-0.1
N.D.
carbon source
Glycolic Acid (C)
-0.0
-0.8
-0.4
-0.0
N.D.
carbon source
carbon source D-Mannose 5mM
-0.0
-0.5
-0.4
-0.2
N.D.
stress
M9 with Methotrexate 0.06 mM 48 hr
+0.0
-0.2
-0.9
-0.0
N.D.
stress
M9 with Methotrexate 0.06 mM 24 hr
-0.0
-0.3
-0.8
+0.0
N.D.
phage
N4_phage 0.009375 MOI TC1
-0.2
-0.3
-0.4
-0.2
N.D.
carbon source
acetate (C)
-0.0
-0.5
-0.6
+0.0
N.D.
stress
Cisplatin 0.1 mg/ml
-0.1
-0.2
-0.9
+0.1
N.D.
no stress control
M9 no stress control; Aerobic 48 hr
-0.1
-0.3
-0.7
-0.0
N.D.
carbon source
carbon source D-Mannose 5mM
-0.2
-0.2
-0.5
-0.1
N.D.
carbon source
acetate (C)
-0.2
-0.4
-0.5
+0.0
N.D.
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.2
-0.8
+0.1
N.D.
stress
3-methyl-1-butanol 1.25 vol%
+0.2
-0.2
-0.7
+0.1
N.D.
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.4
-0.5
-0.3
+0.1
N.D.
carbon source
D-Serine (C)
+0.6
-0.0
-0.6
-0.1
N.D.
phage
T7_phage 0.00015625 MOI TC4
+0.3
+0.7
+0.1
+0.0
N.D.
phage
T7_phage 0.0015625 MOI TC2
-0.1
+0.4
+0.6
+0.3
N.D.
control
PBS_cage_2_mouse_1_Cecum_40 hr
-0.1
+0.6
+0.4
+0.2
N.D.
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.2
+0.2
+0.6
+0.2
N.D.
phage
Papa dilution 10-1 cacl2 maltose
+0.1
+0.2
+0.9
-0.0
N.D.
mzaabcde
mzaABCDE
-0.0
+0.2
+0.8
+0.2
N.D.
phage
T7_phage 0.0015625 MOI TC1
+0.3
+0.5
+0.2
+0.2
N.D.
phage
Ste41
+0.4
+0.4
+1.1
-0.2
N.D.
phage
RB68
+0.5
+0.5
+0.8
-0.0
N.D.
phage
Ste41
+0.5
+0.6
+0.8
-0.2
N.D.
phage
RB68
+0.6
+0.5
+0.8
+0.1
N.D.
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