Fitness for 5 genes in Escherichia coli BW25113

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500 ntgspH and yheH overlap by 4 nucleotidesyheH and gspJ overlap by 8 nucleotidesgspJ and gspK overlap by 8 nucleotidesgspK and yheK are separated by 14 nucleotides b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999 gspH b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373 yheH b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953 gspJ b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929 gspK b3333: yheK - putative general protein secretion protein (VIMSS), at 3,461,944 to 3,463,107 yheK
Group Condition b3329 b3330 b3331 b3332 b3333
gspH yheH gspJ gspK yheK
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.3 -0.2 -0.3 -0.3 -0.3
phage N4_phage 0.009375 MOI TC2 -0.0 -0.5 -0.3 -0.2 -0.3
predation 18 hr rep1_HD100_no_dilution -0.3 -0.4 -0.2 -0.3 -0.2
carbon source carbon source D-Mannose 5mM -0.4 -0.3 +0.0 -0.4 -0.1
phage N4_phage 0.0009375 MOI TC2 -0.3 -0.2 -0.3 -0.2 -0.1
carbon source carbon source D-Glucose 0.25mM -0.4 -0.2 +0.1 -0.5 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.6 -0.2 -0.3 +0.3 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.1 -0.3 -0.2 -0.4
phage R2_N4_T1_phage10 +0.3 -0.6 -0.2 -0.3 +0.0
phage RB68 -0.5 +0.1 -0.2 -0.2 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.2 -0.1 +0.4 -0.1
phage R1_N4_T2_phage8 +0.4 -0.4 -0.0 -0.3 +0.0
stress Chlorite 1.25 mM +0.5 -0.2 -0.3 -0.1 -0.2
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 -0.4 -0.1 +0.3 -0.1
stress .5% DMSO -0.3 -0.1 +0.3 +0.1 +0.4
control LB -0.2 -0.2 +0.3 +0.1 +0.5
carbon source acetate (C) +0.4 -0.2 +0.5 -0.1 +0.2
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.1 +0.4 +0.4 +0.1 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.9 -0.3 +0.0 +0.1 +0.3
control PBS_cage_1_mice _Feces_22 hr +0.2 +0.3 +0.3 +0.2 +0.0
nitrogen source L-Arginine (N) -0.1 +0.2 +1.0 -0.0 +0.1
control PBS_cage_2_mouse_1_Cecum_40 hr +0.0 +0.1 +0.5 +0.3 +0.3
control PBS_cage_2_mice _Feces_40 hr +0.2 -0.0 +0.6 +0.3 +0.2
control PBS_cage_2_mouse_3_Cecum_40 hr +0.3 +0.0 +0.4 +0.2 +0.3
stress LB Anaerobic with Ampicillin 0.00000195313 mM +0.3 +0.3 +0.3 +0.4 +0.0
control PBS_cage_2_mouse_2_Cecum_40 hr +0.4 +0.0 +0.3 +0.3 +0.3
nitrogen source L-Arginine (N) +0.3 +0.1 +1.0 -0.1 +0.0
phage T7_phage 0.00015625 MOI TC3 +0.3 +0.3 -0.0 +0.5 +0.3
control PBS_cage_1_mice _Feces_40 hr +0.5 +0.5 +0.3 +0.3 +0.0
control PBS_cage_1_mice _Feces_16 hr +0.6 +0.3 +0.3 +0.4 +0.0
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