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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
gspG and gspH are separated by 7 nucleotides
gspH and yheH overlap by 4 nucleotides
yheH and gspJ overlap by 8 nucleotides
gspJ and gspK overlap by 8 nucleotides
b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482
gspG
b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999
gspH
b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373
yheH
b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953
gspJ
b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929
gspK
Group
Condition
b3328
b3329
b3330
b3331
b3332
gspG
gspH
yheH
gspJ
gspK
predation
18 hr rep1_HD100_no_dilution
-0.4
-0.3
-0.4
-0.2
-0.3
stress
Tetracycline 0.0008 mg/ml
-0.7
-0.1
-0.3
-0.1
-0.3
nophagecontrol
R1_N4_T1_phage0
-0.4
-0.1
-0.5
-0.2
-0.2
phage
N4_phage 0.009375 MOI TC2
-0.3
-0.0
-0.5
-0.3
-0.2
carbon source
carbon source D-Mannose 5mM
-0.2
-0.4
-0.3
+0.0
-0.4
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.1
-0.3
-0.2
-0.3
-0.3
carbon source
carbon source D-Glucose 0.25mM
-0.2
-0.4
-0.2
+0.1
-0.5
phage
RB68
-0.2
-0.5
+0.1
-0.2
-0.2
phage
R2_N4_T1_phage10
-0.3
+0.3
-0.6
-0.2
-0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.3
-0.6
-0.2
-0.3
+0.3
protonated/deuterated
deuterated glucose in H2O
-0.6
+0.2
-0.2
+0.1
-0.2
phage
R1_N4_T2_phage8
-0.2
+0.4
-0.4
-0.0
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.5
+0.4
-0.4
-0.1
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
-0.4
-0.2
-0.1
+0.4
carbon source
acetate (C)
-0.3
+0.1
-0.2
+0.5
+0.1
stress
Chlorite 1.25 mM
+0.2
+0.5
-0.2
-0.3
-0.1
carbon source
acetate (C)
-0.2
+0.4
-0.2
+0.5
-0.1
phage
T7_phage 0.00015625 MOI TC3
-0.2
+0.3
+0.3
-0.0
+0.5
stress
Benzalkonium Chloride 0.004 mg/ml
+0.3
+0.9
-0.3
+0.0
+0.1
nitrogen source
L-Arginine (N)
-0.1
-0.1
+0.2
+1.0
-0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.7
-0.0
+0.3
-0.1
+0.2
nitrogen source
L-Arginine (N)
-0.2
+0.3
+0.1
+1.0
-0.1
plasmid transformation
vcDART vector transformation; agar assay
+0.4
+0.3
-0.0
+0.3
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.3
+0.4
+0.2
+0.3
+0.1
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.3
+0.4
+0.0
+0.3
+0.3
control
PBS_cage_2_mouse_3_Cecum_40 hr
+0.3
+0.3
+0.0
+0.4
+0.2
control
PBS_cage_2_mice _Feces_40 hr
+0.3
+0.2
-0.0
+0.6
+0.3
control
PBS_cage_1_mice _Feces_40 hr
+0.0
+0.5
+0.5
+0.3
+0.3
control
PBS_cage_1_mice _Feces_16 hr
+0.1
+0.6
+0.3
+0.3
+0.4
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
+0.4
+0.3
+0.3
+0.3
+0.4
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