Fitness for 5 genes in Escherichia coli BW25113

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500 ntgspG and gspH are separated by 7 nucleotidesgspH and yheH overlap by 4 nucleotidesyheH and gspJ overlap by 8 nucleotidesgspJ and gspK overlap by 8 nucleotides b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482 gspG b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999 gspH b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373 yheH b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953 gspJ b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929 gspK
Group Condition b3328 b3329 b3330 b3331 b3332
gspG gspH yheH gspJ gspK
predation 18 hr rep1_HD100_no_dilution -0.4 -0.3 -0.4 -0.2 -0.3
stress Tetracycline 0.0008 mg/ml -0.7 -0.1 -0.3 -0.1 -0.3
nophagecontrol R1_N4_T1_phage0 -0.4 -0.1 -0.5 -0.2 -0.2
phage N4_phage 0.009375 MOI TC2 -0.3 -0.0 -0.5 -0.3 -0.2
carbon source carbon source D-Mannose 5mM -0.2 -0.4 -0.3 +0.0 -0.4
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 -0.3 -0.2 -0.3 -0.3
carbon source carbon source D-Glucose 0.25mM -0.2 -0.4 -0.2 +0.1 -0.5
phage RB68 -0.2 -0.5 +0.1 -0.2 -0.2
phage R2_N4_T1_phage10 -0.3 +0.3 -0.6 -0.2 -0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -0.6 -0.2 -0.3 +0.3
protonated/deuterated deuterated glucose in H2O -0.6 +0.2 -0.2 +0.1 -0.2
phage R1_N4_T2_phage8 -0.2 +0.4 -0.4 -0.0 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.5 +0.4 -0.4 -0.1 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -0.4 -0.2 -0.1 +0.4
carbon source acetate (C) -0.3 +0.1 -0.2 +0.5 +0.1
stress Chlorite 1.25 mM +0.2 +0.5 -0.2 -0.3 -0.1
carbon source acetate (C) -0.2 +0.4 -0.2 +0.5 -0.1
phage T7_phage 0.00015625 MOI TC3 -0.2 +0.3 +0.3 -0.0 +0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.3 +0.9 -0.3 +0.0 +0.1
nitrogen source L-Arginine (N) -0.1 -0.1 +0.2 +1.0 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.7 -0.0 +0.3 -0.1 +0.2
nitrogen source L-Arginine (N) -0.2 +0.3 +0.1 +1.0 -0.1
plasmid transformation vcDART vector transformation; agar assay +0.4 +0.3 -0.0 +0.3 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 +0.4 +0.2 +0.3 +0.1
control PBS_cage_2_mouse_2_Cecum_40 hr +0.3 +0.4 +0.0 +0.3 +0.3
control PBS_cage_2_mouse_3_Cecum_40 hr +0.3 +0.3 +0.0 +0.4 +0.2
control PBS_cage_2_mice _Feces_40 hr +0.3 +0.2 -0.0 +0.6 +0.3
control PBS_cage_1_mice _Feces_40 hr +0.0 +0.5 +0.5 +0.3 +0.3
control PBS_cage_1_mice _Feces_16 hr +0.1 +0.6 +0.3 +0.3 +0.4
stress LB Anaerobic with Ampicillin 0.00000195313 mM +0.4 +0.3 +0.3 +0.3 +0.4
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