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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
mscL and yhdL overlap by 4 nucleotides
yhdL and zntR are separated by 55 nucleotides
zntR and yhdN are separated by 10 nucleotides
yhdN and rplQ are separated by 106 nucleotides
b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456
mscL
b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671
yhdL
b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152
zntR
b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531
yhdN
b3294: rplQ - 50S ribosomal protein L17 (NCBI), at 3,437,638 to 3,438,021
rplQ
Group
Condition
b3291
b4550
b3292
b3293
b3294
mscL
yhdL
zntR
yhdN
rplQ
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.2
-0.2
-2.3
-0.2
N.D.
retron_ec_48_control
retron_Ec_48_control
-1.3
-0.6
-0.2
-0.8
N.D.
gfp_control
GFP_control
-1.3
-0.2
-0.3
-0.9
N.D.
gfp_control
GFP_control
-1.1
-0.1
-0.6
-0.8
N.D.
gfp_control
GFP_control
-1.2
-0.7
+0.0
-0.6
N.D.
carbon source
acetate (C)
-0.1
+0.1
-2.3
+0.1
N.D.
carbon source
acetate (C)
+0.1
+0.2
-3.1
+0.4
N.D.
stress
LB Anaerobic with Zinc Pyrithione 0.00000125 mM
-0.1
+0.1
-2.4
+0.1
N.D.
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.2
+0.4
-2.8
+0.3
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.00000125 mM
+0.2
+0.1
-2.4
+0.2
N.D.
nitrogen source
Putrescine (N)
-0.0
+0.1
-2.3
+0.3
N.D.
stress
LB Anaerobic with Zinc Pyrithione 0.00000125 mM
-0.0
+0.1
-2.3
+0.4
N.D.
stress
LB Aerobic with Doxycycline hyclate 0.00000625 mM
+0.3
-2.1
-0.2
+0.3
N.D.
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
+0.1
-1.8
-0.6
+0.6
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.00000125 mM
+0.2
+0.5
-2.5
+0.2
N.D.
stress
LB Anaerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.0
+0.2
-2.0
+0.3
N.D.
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.1
+0.6
-2.0
+0.1
N.D.
carbon source
carbon source D-Glucose 0.25mM
+0.2
-1.3
-0.9
+0.6
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.5
-1.0
-0.9
+1.1
N.D.
stress
2-nitrobenzenesulfonyl fluoride
-0.0
-0.1
-1.8
+0.7
N.D.
nitrogen source
Putrescine (N)
+0.0
+0.4
-2.1
+0.4
N.D.
carbon source
a-Ketoglutaric (C)
+0.2
+0.3
-1.8
+0.5
N.D.
carbon source
Glycolic Acid (C)
+0.0
+0.3
-1.7
+0.6
N.D.
carbon source
L-Malic (C)
+0.1
+0.3
-1.8
+0.7
N.D.
carbon source
a-Ketoglutaric (C)
+0.0
+0.3
-1.7
+0.7
N.D.
carbon source
Glycolic Acid (C)
+0.2
+0.3
-1.8
+0.8
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.1
+2.1
-2.4
+1.0
N.D.
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.3
+2.0
-2.1
+1.6
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.0
+2.4
-2.0
+1.9
N.D.
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.8
+1.9
-1.5
+1.5
N.D.
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