Fitness for 5 genes in Escherichia coli BW25113

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500 ntdef and fmt are separated by 14 nucleotidesfmt and rsmB are separated by 45 nucleotidesrsmB and trkA are separated by 21 nucleotidestrkA and mscL are separated by 129 nucleotides b3287: def - peptide deformylase (NCBI), at 3,431,712 to 3,432,221 def b3288: fmt - methionyl-tRNA formyltransferase (NCBI), at 3,432,236 to 3,433,183 fmt b3289: rsmB - 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI), at 3,433,229 to 3,434,518 rsmB b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916 trkA b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456 mscL
Group Condition b3287 b3288 b3289 b3290 b3291
def fmt rsmB trkA mscL
carbon source Glycolic Acid (C) N.D. N.D. -1.7 -2.7 +0.0
phage Phage_N4_cage_4_mice _Feces_40 hr N.D. N.D. -0.3 -3.8 +0.1
carbon source Glycolic Acid (C) N.D. N.D. -1.5 -2.6 +0.2
carbon source acetate (C) N.D. N.D. -1.0 -2.6 -0.1
motility outer cut, LB soft agar motility assay N.D. N.D. -0.2 -3.4 -0.1
stress hexanol 0.31 vol% N.D. N.D. -0.7 -3.0 -0.1
stress Spectinomycin 0.00625 mg/ml N.D. N.D. -1.2 -2.2 -0.1
carbon source acetate (C) N.D. N.D. -1.2 -2.5 +0.1
stress 3-methyl-3-butenol 0.625 vol% N.D. N.D. -0.6 -3.0 +0.1
stress Spectinomycin 0.0125 mg/ml N.D. N.D. -1.6 -2.0 +0.1
stress ethyl acetate 5 vol% N.D. N.D. -0.6 -2.8 -0.0
motility inner cut, LB soft agar motility assay N.D. N.D. -0.6 -2.8 +0.1
stress ethyl acetate 5 vol% N.D. N.D. -0.6 -2.8 +0.0
stress 3-methyl-2-butenol 0.625 vol% N.D. N.D. -0.3 -3.0 +0.1
stress 3-methyl-1-butanol 0.625 vol% N.D. N.D. -0.5 -2.9 +0.2
stress 3-methyl-1-butanol 0.625 vol% N.D. N.D. -0.4 -2.8 +0.2
carbon source D-Lactate (C) N.D. N.D. -0.4 -2.8 +0.2
stress 3-methyl-2-butenol 1.25 vol% N.D. N.D. -0.0 -3.1 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. -2.3 +0.8 -1.1
control PBS_cage_2_mouse_3_Cecum_40 hr N.D. N.D. -0.5 -2.5 +0.5
stress Chlorite 1.25 mM N.D. N.D. -0.5 +2.7 -0.2
stress Doxycycline hyclate 0.001 mg/ml N.D. N.D. -0.5 +3.3 -0.2
stress Cephalothin 0.01 mg/ml N.D. N.D. -0.4 +3.5 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. +0.5 +2.7 -0.2
stress LB Anaerobic with Nalidixic 0.0000078125 mM N.D. N.D. +0.4 +3.1 +0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. +2.9 +0.8 +0.0
stress LB Anaerobic with Nalidixic 0.0000078125 mM N.D. N.D. +0.4 +3.4 -0.0
stress Bacitracin 1 mg/ml N.D. N.D. +1.6 +2.2 +0.2
stress LB Aerobic with Irgasan 0.0000003125 mM N.D. N.D. +2.4 +1.3 +0.3
stress Nalidixic 0.006 mg/ml N.D. N.D. +0.7 +5.4 +0.0
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