Fitness for 5 genes in Escherichia coli BW25113

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500 ntaccC and yhdT are separated by 108 nucleotidesyhdT and panF overlap by 11 nucleotidespanF and prmA are separated by 11 nucleotidesprmA and dusB are separated by 328 nucleotides b3256: accC - acetyl-CoA carboxylase (NCBI), at 3,403,939 to 3,405,288 accC b3257: yhdT - conserved inner membrane protein (NCBI), at 3,405,397 to 3,405,639 yhdT b3258: panF - sodium/pantothenate symporter (VIMSS), at 3,405,629 to 3,407,080 panF b3259: prmA - ribosomal protein L11 methyltransferase (NCBI), at 3,407,092 to 3,407,973 prmA b3260: dusB - tRNA-dihydrouridine synthase B (NCBI), at 3,408,302 to 3,409,267 dusB
Group Condition b3256 b3257 b3258 b3259 b3260
accC yhdT panF prmA dusB
phage Δdbn_10 N.D. -0.8 -0.1 -0.6 -3.6
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. -1.2 -0.5 -1.1 -1.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. -0.6 -0.7 -0.8 -1.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. -1.4 -0.9 -0.6 -0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. -0.2 -0.3 -1.4 -1.2
carbon source carbon source D-Glucose 5mM N.D. -1.5 -0.4 +0.4 +0.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.5 -1.2 -0.8 +2.4
stress Cephalothin 0.01 mg/ml N.D. -0.6 -0.5 -0.2 +1.9
phage T7_phage 0.0015625 MOI TC2 N.D. +0.4 +0.6 -1.3 +1.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.7 -0.4 -0.9 +2.8
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.7 +1.0 +0.5 -1.2
carbon source Acetate (C) N.D. -0.3 -0.6 +0.2 +1.9
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.9 +1.1 +0.2 -1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.4 +1.4 -2.1 +1.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.5 +1.2 -1.1 +0.5
stress Spectinomycin 0.00625 mg/ml N.D. +0.4 +0.5 -0.6 +1.9
carbon source L-Malic (C) N.D. +0.2 +0.7 -0.4 +2.1
plasmid transformation vcDART vector transformation; agar assay N.D. +1.0 +0.8 +0.0 +1.1
plasmid transformation vcDART vector transformation; agar assay N.D. +1.3 +1.2 +0.2 +0.4
carbon source L-Malic (C) N.D. +0.2 +0.8 +0.1 +2.2
plasmid transformation vcDART vector transformation; agar assay N.D. +1.4 +1.2 +0.4 +0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.9 +1.3 -0.6 +1.0
stress LB Aerobic with Novobiocin 0.0000625 mM N.D. +0.4 +0.9 +0.5 +2.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +2.0 +1.7 -0.9 +1.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.5 +1.2 -0.0 +1.3
stress Spectinomycin 0.0125 mg/ml N.D. +1.3 +0.8 -0.7 +3.3
stress LB Aerobic with Novobiocin 0.0000625 mM N.D. +1.2 +1.0 +0.3 +2.2
stress Benzalkonium Chloride 0.004 mg/ml N.D. +0.7 +1.1 -0.5 +3.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. +2.1 +1.4 -0.4 +2.9
stress LB Aerobic with Irgasan 0.0000003125 mM N.D. +4.1 +3.6 +0.8 -1.3
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