Fitness for 5 genes in Escherichia coli BW25113

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500 ntaccB and accC are separated by 10 nucleotidesaccC and yhdT are separated by 108 nucleotidesyhdT and panF overlap by 11 nucleotidespanF and prmA are separated by 11 nucleotides b3255: accB - acetyl-CoA carboxylase (NCBI), at 3,403,458 to 3,403,928 accB b3256: accC - acetyl-CoA carboxylase (NCBI), at 3,403,939 to 3,405,288 accC b3257: yhdT - conserved inner membrane protein (NCBI), at 3,405,397 to 3,405,639 yhdT b3258: panF - sodium/pantothenate symporter (VIMSS), at 3,405,629 to 3,407,080 panF b3259: prmA - ribosomal protein L11 methyltransferase (NCBI), at 3,407,092 to 3,407,973 prmA
Group Condition b3255 b3256 b3257 b3258 b3259
accB accC yhdT panF prmA
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. -1.4 -0.9 -0.6
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. -1.2 -0.5 -1.1
phage Ste41 N.D. N.D. -0.0 -0.4 -2.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. -0.5 -1.2 -0.8
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. -0.7 -0.8 -0.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. N.D. -0.6 -0.7 -0.8
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. -0.7 -0.4 -0.9
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. +0.0 -0.3 -1.7
stress Chlorite 1.25 mM N.D. N.D. -0.9 -1.2 +0.3
carbon source carbon source D-Glucose 5mM N.D. N.D. -1.5 -0.4 +0.4
control PBS_cage_1_mice _Feces_16 hr N.D. N.D. -1.1 +0.4 -0.5
phage T7_phage 0.0015625 MOI TC2 N.D. N.D. +0.4 +0.6 -1.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +1.4 +1.4 -2.1
stress Spectinomycin 0.0125 mg/ml N.D. N.D. +1.3 +0.8 -0.7
stress Benzalkonium Chloride 0.004 mg/ml N.D. N.D. +0.7 +1.1 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +1.5 +1.2 -1.1
stress LB Aerobic with Spiramycin 0.000125 mM N.D. N.D. +0.9 +0.9 -0.2
stress Bacitracin 1 mg/ml N.D. N.D. +1.0 +1.0 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. +0.7 +1.0 +0.5
stress Trimethoprim 0.00125 mM N.D. N.D. +0.9 +0.9 +0.4
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. +0.9 +1.1 +0.2
stress Trimethoprim 0.0025 mM N.D. N.D. +0.5 +1.0 +0.7
stress LB Aerobic with Novobiocin 0.0000625 mM N.D. N.D. +1.2 +1.0 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +1.9 +1.3 -0.6
plasmid transformation vcDART vector transformation; agar assay N.D. N.D. +1.3 +1.2 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +1.5 +1.2 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +2.0 +1.7 -0.9
plasmid transformation vcDART vector transformation; agar assay N.D. N.D. +1.4 +1.2 +0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. +2.1 +1.4 -0.4
stress LB Aerobic with Irgasan 0.0000003125 mM N.D. N.D. +4.1 +3.6 +0.8
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