Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhdP and cafA are separated by 67 nucleotidescafA and yhdE overlap by 11 nucleotidesyhdE and mreD are separated by 8 nucleotidesmreD and mreC overlap by 1 nucleotides b4472: yhdP - conserved membrane protein, predicted transporter (NCBI), at 3,390,480 to 3,394,280 yhdP b3247: cafA - bundles of cytoplasmic filaments (VIMSS), at 3,394,348 to 3,395,817 cafA b3248: yhdE - Maf-like protein (NCBI), at 3,395,807 to 3,396,400 yhdE b3249: mreD - cell wall structural complex MreBCD transmembrane component MreD (NCBI), at 3,396,409 to 3,396,897 mreD b3250: mreC - cell wall structural complex MreBCD transmembrane component MreC (NCBI), at 3,396,897 to 3,398,000 mreC
Group Condition b4472 b3247 b3248 b3249 b3250
yhdP cafA yhdE mreD mreC
stress LB Aerobic with Ramoplanin 0.000125 mM -6.0 -0.9 +0.7 N.D. -1.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -5.1 -2.3 +0.4 N.D. +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -5.9 -1.8 +0.2 N.D. +0.9
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -4.9 -1.9 +0.8 N.D. -0.5
stress Bacitracin 0.5 mg/ml -4.2 -1.7 +0.0 N.D. -0.0
stress Bacitracin 1 mg/ml -3.9 -2.6 +1.3 N.D. -0.3
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -4.0 -1.0 +0.0 N.D. -0.4
carbon source D-Maltose (C) -0.4 -3.2 +0.4 N.D. -2.1
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -3.9 -1.1 +0.1 N.D. -0.2
stress LB Anaerobic with Ramoplanin 0.0000625 mM -3.0 -0.9 -0.3 N.D. -0.7
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.6 +0.2 -0.1 N.D. -3.5
stress Benzalkonium Chloride 0.004 mg/ml -3.5 +0.8 +0.4 N.D. -0.9
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.1 -4.1 +1.3 N.D. +0.2
carbon source Glycolic Acid (C) -0.8 -3.7 +2.3 N.D. +0.2
carbon source D-Ribose (C) -0.6 -2.4 +1.3 N.D. -0.2
carbon source L-Malic (C) -0.8 -2.0 +1.1 N.D. -0.3
carbon source L-Malic (C) -0.7 -2.4 +1.1 N.D. +0.1
carbon source D-Ribose (C) -0.8 -2.7 +1.2 N.D. +0.5
carbon source Glycolic Acid (C) -0.9 -3.9 +2.6 N.D. +0.6
carbon source succinate (C) -0.5 -2.2 +1.6 N.D. +0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -1.7 +0.3 N.D. +2.6
stress Trimethoprim 0.00125 mM -0.5 +2.6 +0.6 N.D. -0.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.5 -1.3 +1.5 N.D. +1.5
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 +2.8 +0.5 N.D. -1.2
stress Trimethoprim 0.0025 mM -0.5 +3.2 +0.7 N.D. -0.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.3 +3.3 +0.4 N.D. -0.8
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.2 +3.3 +0.3 N.D. -0.6
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.9 +4.1 +0.9 N.D. -1.6
stress octanol 0.08 vol% +3.6 -0.6 +0.1 N.D. +3.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +5.3 +2.1 +1.9 N.D. -0.8
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