Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhcR and aaeR are separated by 182 nucleotidesaaeR and tldD are separated by 133 nucleotidestldD and yhdP are separated by 429 nucleotidesyhdP and cafA are separated by 67 nucleotides b3242: yhcR - orf, hypothetical protein (VIMSS), at 3,387,156 to 3,387,359 yhcR b3243: aaeR - predicted DNA-binding transcriptional regulator, efflux system (NCBI), at 3,387,542 to 3,388,471 aaeR b3244: tldD - predicted peptidase (NCBI), at 3,388,605 to 3,390,050 tldD b4472: yhdP - conserved membrane protein, predicted transporter (NCBI), at 3,390,480 to 3,394,280 yhdP b3247: cafA - bundles of cytoplasmic filaments (VIMSS), at 3,394,348 to 3,395,817 cafA
Group Condition b3242 b3243 b3244 b4472 b3247
yhcR aaeR tldD yhdP cafA
stress LB Aerobic with Ramoplanin 0.000125 mM -1.3 -0.7 +0.2 -5.9 -1.8
stress LB Aerobic with Ramoplanin 0.000125 mM -1.8 +0.2 +0.1 -6.0 -0.9
stress Bacitracin 1 mg/ml -1.3 +0.0 -0.1 -3.9 -2.6
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.0 +0.0 -0.4 -5.1 -2.3
stress Bacitracin 0.5 mg/ml -0.6 -0.2 -0.1 -4.2 -1.7
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.7 +0.2 -0.3 -4.9 -1.9
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.1 +0.1 +0.0 -3.9 -1.1
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.2 -0.1 +0.0 -4.0 -1.0
carbon source Glycolic Acid (C) +0.7 -0.0 -0.6 -0.9 -3.9
stress Benzalkonium Chloride 0.004 mg/ml -2.3 +0.7 -0.2 -3.5 +0.8
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.4 +0.3 -0.1 -0.1 -4.1
carbon source Glycolic Acid (C) +0.8 +0.2 -0.7 -0.8 -3.7
stress Aluminum chloride 5 mM -0.6 -0.6 -0.5 -0.9 -1.5
carbon source D-Ribose (C) -0.0 +0.1 -0.4 -0.8 -2.7
stress LB Anaerobic with Ramoplanin 0.0000625 mM +0.2 +0.2 -0.0 -3.0 -0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.4 -1.4 -0.6 -0.4 +0.2
stress LB Anaerobic with Ramoplanin 0.0000625 mM -0.2 +0.1 +0.1 -2.8 -0.7
carbon source D-Maltose (C) +0.2 +0.1 -0.2 -0.4 -3.2
carbon source D-Maltose (C) +0.2 +0.2 -0.4 -0.5 -2.8
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.3 +0.6 -1.0 +0.2 -1.7
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.8 -0.5 -0.6 +1.7 +0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.7 +0.4 -0.5 +2.1 +0.4
stress Trimethoprim 0.00125 mM -0.9 +0.1 +0.8 -0.5 +2.6
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.8 -0.6 +0.1 +0.2 +3.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.4 -0.2 +0.1 +0.3 +2.8
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.6 -0.3 +0.1 +0.3 +3.3
stress octanol 0.08 vol% +0.0 -0.4 +0.3 +3.6 -0.6
stress Trimethoprim 0.0025 mM -0.7 +0.0 +1.0 -0.5 +3.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.8 +0.1 +0.2 +0.9 +4.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 -0.4 +0.1 +5.3 +2.1
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