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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhcR and aaeR are separated by 182 nucleotides
aaeR and tldD are separated by 133 nucleotides
tldD and yhdP are separated by 429 nucleotides
yhdP and cafA are separated by 67 nucleotides
b3242: yhcR - orf, hypothetical protein (VIMSS), at 3,387,156 to 3,387,359
yhcR
b3243: aaeR - predicted DNA-binding transcriptional regulator, efflux system (NCBI), at 3,387,542 to 3,388,471
aaeR
b3244: tldD - predicted peptidase (NCBI), at 3,388,605 to 3,390,050
tldD
b4472: yhdP - conserved membrane protein, predicted transporter (NCBI), at 3,390,480 to 3,394,280
yhdP
b3247: cafA - bundles of cytoplasmic filaments (VIMSS), at 3,394,348 to 3,395,817
cafA
Group
Condition
b3242
b3243
b3244
b4472
b3247
yhcR
aaeR
tldD
yhdP
cafA
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.3
-0.7
+0.2
-5.9
-1.8
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.8
+0.2
+0.1
-6.0
-0.9
stress
Bacitracin 1 mg/ml
-1.3
+0.0
-0.1
-3.9
-2.6
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.0
+0.0
-0.4
-5.1
-2.3
stress
Bacitracin 0.5 mg/ml
-0.6
-0.2
-0.1
-4.2
-1.7
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.7
+0.2
-0.3
-4.9
-1.9
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
-0.1
+0.1
+0.0
-3.9
-1.1
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
+0.2
-0.1
+0.0
-4.0
-1.0
carbon source
Glycolic Acid (C)
+0.7
-0.0
-0.6
-0.9
-3.9
stress
Benzalkonium Chloride 0.004 mg/ml
-2.3
+0.7
-0.2
-3.5
+0.8
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.4
+0.3
-0.1
-0.1
-4.1
carbon source
Glycolic Acid (C)
+0.8
+0.2
-0.7
-0.8
-3.7
stress
Aluminum chloride 5 mM
-0.6
-0.6
-0.5
-0.9
-1.5
carbon source
D-Ribose (C)
-0.0
+0.1
-0.4
-0.8
-2.7
stress
LB Anaerobic with Ramoplanin 0.0000625 mM
+0.2
+0.2
-0.0
-3.0
-0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.4
-1.4
-0.6
-0.4
+0.2
stress
LB Anaerobic with Ramoplanin 0.0000625 mM
-0.2
+0.1
+0.1
-2.8
-0.7
carbon source
D-Maltose (C)
+0.2
+0.1
-0.2
-0.4
-3.2
carbon source
D-Maltose (C)
+0.2
+0.2
-0.4
-0.5
-2.8
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.3
+0.6
-1.0
+0.2
-1.7
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.8
-0.5
-0.6
+1.7
+0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.7
+0.4
-0.5
+2.1
+0.4
stress
Trimethoprim 0.00125 mM
-0.9
+0.1
+0.8
-0.5
+2.6
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.8
-0.6
+0.1
+0.2
+3.3
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.4
-0.2
+0.1
+0.3
+2.8
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.6
-0.3
+0.1
+0.3
+3.3
stress
octanol 0.08 vol%
+0.0
-0.4
+0.3
+3.6
-0.6
stress
Trimethoprim 0.0025 mM
-0.7
+0.0
+1.0
-0.5
+3.2
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.8
+0.1
+0.2
+0.9
+4.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.2
-0.4
+0.1
+5.3
+2.1
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