Fitness for 5 genes in Escherichia coli BW25113

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500 ntrpsI and rplM are separated by 15 nucleotidesrplM and yhcM are separated by 218 nucleotidesyhcM and yhcB are separated by 193 nucleotidesyhcB and degQ are separated by 153 nucleotides b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229 rpsI b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673 rplM b3232: yhcM - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,376,892 to 3,378,019 yhcM b3233: yhcB - orf, hypothetical protein (VIMSS), at 3,378,213 to 3,378,611 yhcB b3234: degQ - serine endoprotease, periplasmic (NCBI), at 3,378,765 to 3,380,132 degQ
Group Condition b3230 b3231 b3232 b3233 b3234
rpsI rplM yhcM yhcB degQ
carbon source succinate (C) N.D. N.D. -3.7 N.D. +0.3
carbon source succinate (C) N.D. N.D. -2.9 N.D. +0.1
carbon source Ying_OrganicAcid7 rep C; time point 3 N.D. N.D. -2.2 N.D. -0.2
carbon source Ying_OrganicAcid7 rep B; time point 3 N.D. N.D. -2.3 N.D. -0.1
carbon source Ying_OrganicAcid7 rep A; time point 3 N.D. N.D. -2.1 N.D. -0.1
carbon source a-Ketoglutaric (C) N.D. N.D. -2.3 N.D. +0.2
carbon source a-Ketoglutaric (C) N.D. N.D. -2.2 N.D. +0.4
carbon source Ying_OrganicAcid7 rep C; time point 2 N.D. N.D. -1.7 N.D. -0.1
carbon source Ying_OrganicAcid7 rep B; time point 2 N.D. N.D. -1.7 N.D. -0.0
carbon source casaminos (C) N.D. N.D. -1.6 N.D. +0.1
carbon source Ying_OrganicAcid7 rep A; time point 2 N.D. N.D. -1.5 N.D. -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 N.D. N.D. -1.3 N.D. -0.2
carbon source Ying_AminoAcid20 rep B; time point 3 N.D. N.D. -1.3 N.D. +0.0
carbon source casaminos (C) N.D. N.D. -1.3 N.D. +0.1
carbon source Ying_AminoAcid20 rep A; time point 3 N.D. N.D. -1.2 N.D. +0.2
stress ethyl acetate 5 vol% N.D. N.D. -1.1 N.D. +0.2
ecofab LB in EcoFAB_3.5mL N.D. N.D. -0.5 N.D. +0.8
carbon source carbon source D-Glucose 5mM N.D. N.D. -0.6 N.D. +1.3
phage T7_phage 0.00015625 MOI TC4 N.D. N.D. +1.3 N.D. -0.2
carbon source carbon source D-Mannose 5mM N.D. N.D. -0.2 N.D. +1.5
plasmid transformation vcDART vector transformation; agar assay N.D. N.D. +1.4 N.D. -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +1.3 N.D. +0.2
plasmid transformation mariner vector transformation; agar assay N.D. N.D. +1.4 N.D. +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +1.6 N.D. -0.0
plasmid transformation vcDART vector transformation; agar assay N.D. N.D. +1.7 N.D. -0.1
plasmid transformation vcDART vector transformation; agar assay N.D. N.D. +1.8 N.D. +0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +2.0 N.D. +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +2.1 N.D. +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +3.1 N.D. -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +3.3 N.D. -0.1
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