Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhbJ and npr overlap by 4 nucleotidesnpr and yrbL are separated by 213 nucleotidesyrbL and mtgA overlap by 4 nucleotidesmtgA and yhbL overlap by 4 nucleotides b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991 yhbJ b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260 npr b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106 yrbL b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831 mtgA b3209: yhbL - sigma cross-reacting protein 27A (SCRP-27A) (VIMSS), at 3,347,828 to 3,348,481 yhbL
Group Condition b3205 b3206 b3207 b3208 b3209
yhbJ npr yrbL mtgA yhbL
motility outer cut, LB soft agar motility assay -4.1 -2.6 -0.3 -0.0 -0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -4.3 -0.3 -0.4 -0.0 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -2.5 -0.8 -1.6 +0.1 +0.4
motility outer cut, LB soft agar motility assay -2.2 -1.8 -0.4 +0.3 +0.1
stress octanol 0.08 vol% +0.9 +0.8 -4.2 -0.3 -0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.5 +0.6 -2.3 -0.2 +0.3
carbon source carbon source D-Glucose 0.25mM -2.7 +0.9 +0.1 -0.5 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.8 +1.6 -0.2 -0.4 -0.3
carbon source Ying_Nucleobase14 rep B; time point 3 -3.1 +1.1 -0.2 +0.2 +0.1
carbon source carbon source D-Mannose 5mM -2.9 +1.3 -0.3 +0.4 +0.0
carbon source Glycolic Acid (C) -3.1 +1.6 +0.1 +0.3 -0.3
carbon source Glycolic Acid (C) -3.2 +2.1 +0.2 +0.1 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.0 +2.3 +0.2 -0.8 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +3.6 +0.5 -4.0 -0.5 +0.3
stress Doxycycline hyclate 0.001 mg/ml +1.7 -1.0 -1.3 +0.2 +0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -2.1 +1.8 +0.1 +0.3 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.0 +2.3 +0.3 +0.1 -0.1
stress Bacitracin 1 mg/ml +1.6 -1.5 +0.9 +0.3 -0.4
stress LB Anaerobic with Doxycycline hyclate 0.000000390625 mM +3.0 -0.8 -0.8 +0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.9 +3.2 +0.6 -0.4 +0.1
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +2.8 -0.5 -0.9 -0.1 +0.1
carbon source carbon source D-Mannose 20mM -1.4 +2.5 +0.5 +0.0 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.9 +3.5 +0.8 -0.4 -0.2
carbon source carbon source D-Glucose 20mM -1.9 +3.7 +0.3 +0.2 -0.2
stress Aluminum chloride 5 mM -1.7 -0.1 +3.4 +0.7 +0.1
carbon source carbon source D-Glucose 20mM -0.8 +3.4 +0.4 -0.4 +0.0
phage T7_phage 0.0015625 MOI TC2 +3.0 +0.3 +1.4 -0.2 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +2.7 +0.2 +1.3 +0.2 +0.1
phage T7_phage 0.00015625 MOI TC4 +4.0 -0.0 +1.5 +0.1 +0.3
stress LB Aerobic with Irgasan 0.0000003125 mM +8.4 +1.6 +0.1 +0.3 -0.3
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