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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhbJ and npr overlap by 4 nucleotides
npr and yrbL are separated by 213 nucleotides
yrbL and mtgA overlap by 4 nucleotides
mtgA and yhbL overlap by 4 nucleotides
b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991
yhbJ
b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260
npr
b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106
yrbL
b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831
mtgA
b3209: yhbL - sigma cross-reacting protein 27A (SCRP-27A) (VIMSS), at 3,347,828 to 3,348,481
yhbL
Group
Condition
b3205
b3206
b3207
b3208
b3209
yhbJ
npr
yrbL
mtgA
yhbL
motility
outer cut, LB soft agar motility assay
-4.1
-2.6
-0.3
-0.0
-0.0
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-4.3
-0.3
-0.4
-0.0
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-2.5
-0.8
-1.6
+0.1
+0.4
motility
outer cut, LB soft agar motility assay
-2.2
-1.8
-0.4
+0.3
+0.1
stress
octanol 0.08 vol%
+0.9
+0.8
-4.2
-0.3
-0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.5
+0.6
-2.3
-0.2
+0.3
carbon source
carbon source D-Glucose 0.25mM
-2.7
+0.9
+0.1
-0.5
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.8
+1.6
-0.2
-0.4
-0.3
carbon source
Ying_Nucleobase14 rep B; time point 3
-3.1
+1.1
-0.2
+0.2
+0.1
carbon source
carbon source D-Mannose 5mM
-2.9
+1.3
-0.3
+0.4
+0.0
carbon source
Glycolic Acid (C)
-3.1
+1.6
+0.1
+0.3
-0.3
carbon source
Glycolic Acid (C)
-3.2
+2.1
+0.2
+0.1
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.0
+2.3
+0.2
-0.8
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+3.6
+0.5
-4.0
-0.5
+0.3
stress
Doxycycline hyclate 0.001 mg/ml
+1.7
-1.0
-1.3
+0.2
+0.3
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-2.1
+1.8
+0.1
+0.3
-0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.0
+2.3
+0.3
+0.1
-0.1
stress
Bacitracin 1 mg/ml
+1.6
-1.5
+0.9
+0.3
-0.4
stress
LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
+3.0
-0.8
-0.8
+0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.9
+3.2
+0.6
-0.4
+0.1
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
+2.8
-0.5
-0.9
-0.1
+0.1
carbon source
carbon source D-Mannose 20mM
-1.4
+2.5
+0.5
+0.0
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.9
+3.5
+0.8
-0.4
-0.2
carbon source
carbon source D-Glucose 20mM
-1.9
+3.7
+0.3
+0.2
-0.2
stress
Aluminum chloride 5 mM
-1.7
-0.1
+3.4
+0.7
+0.1
carbon source
carbon source D-Glucose 20mM
-0.8
+3.4
+0.4
-0.4
+0.0
phage
T7_phage 0.0015625 MOI TC2
+3.0
+0.3
+1.4
-0.2
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+2.7
+0.2
+1.3
+0.2
+0.1
phage
T7_phage 0.00015625 MOI TC4
+4.0
-0.0
+1.5
+0.1
+0.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
+8.4
+1.6
+0.1
+0.3
-0.3
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