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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhbY and greA are separated by 155 nucleotides
greA and dacB are separated by 247 nucleotides
dacB and obgE are separated by 185 nucleotides
obgE and yhbE are separated by 15 nucleotides
b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105
yhbY
b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737
greA
b3182: dacB - D-alanyl-D-alanine carboxypeptidase (NCBI), at 3,326,985 to 3,328,418
dacB
b3183: obgE - GTPase involved in cell partioning and DNA repair (NCBI), at 3,328,604 to 3,329,776
obgE
b3184: yhbE - conserved inner membrane protein (NCBI), at 3,329,792 to 3,330,757
yhbE
Group
Condition
b3180
b3181
b3182
b3183
b3184
yhbY
greA
dacB
obgE
yhbE
gfp_control
GFP_control
-0.9
-8.1
+0.2
-3.4
-0.6
motility
outer cut, LB soft agar motility assay
-2.6
-4.2
-0.1
-3.5
-0.7
sir2-hera
SIR2-HerA
-1.0
-6.9
+0.4
-2.9
-0.6
gfp_control
GFP_control
-1.4
-6.4
+0.5
-3.0
-0.5
phage
T7_phage 0.00015625 MOI TC2
-0.5
-6.7
+0.0
-2.9
-0.5
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.8
-2.8
-3.7
-1.6
-0.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.1
-2.4
-3.9
-1.5
-0.5
mzaabcde
mzaABCDE
-1.0
-4.7
+0.2
-3.1
-0.5
drt_type_3
DRT_type_3
-1.0
-4.3
+0.3
-3.4
-0.6
mzaabcde
mzaABCDE
-1.2
-4.6
+0.2
-3.1
-0.4
mzaabcde
mzaABCDE
-1.2
-4.3
+0.4
-3.1
-0.6
retron_ec_48_control
retron_Ec_48_control
-1.3
-4.6
+0.4
-3.3
-0.2
ppi
ppI
-1.2
-4.2
+0.4
-3.4
-0.5
drt_type_3
DRT_type_3
-1.2
-4.2
+0.4
-3.4
-0.4
sir2-hera
SIR2-HerA
-1.0
-3.8
+0.3
-3.5
-0.4
drt_type_3
DRT_type_3
-0.9
-4.1
+0.4
-3.3
-0.3
ppi
ppI
-0.9
-3.5
+0.3
-3.4
-0.5
sir2-hera
SIR2-HerA
-1.0
-3.3
+0.4
-3.3
-0.5
phage
T7_phage 0.00015625 MOI TC4
-1.4
-4.4
+0.4
-1.7
-0.7
phage
T7_phage 0.000015625 MOI TC3
-0.2
-4.4
+0.1
-3.0
-0.2
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-0.2
-0.8
+0.1
-6.3
-0.3
ppi
ppI
-1.1
-3.0
+0.3
-3.4
-0.4
retron_ec_48_control
retron_Ec_48_control
-0.8
-3.3
+0.4
-3.3
-0.5
retron_ec_48_control
retron_Ec_48_control
-0.8
-3.0
+0.4
-3.4
-0.5
gfp_control
GFP_control
-1.3
-2.5
+0.4
-3.1
-0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+4.5
-2.7
-4.3
-1.2
-0.8
stress
Benzalkonium Chloride 0.004 mg/ml
-0.8
+6.4
-0.3
+0.7
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
+3.5
+0.2
+3.7
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.7
+3.5
+0.2
+3.3
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.0
+4.6
+0.1
+1.9
+0.6
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