Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhbY and greA are separated by 155 nucleotidesgreA and dacB are separated by 247 nucleotidesdacB and obgE are separated by 185 nucleotidesobgE and yhbE are separated by 15 nucleotides b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105 yhbY b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737 greA b3182: dacB - D-alanyl-D-alanine carboxypeptidase (NCBI), at 3,326,985 to 3,328,418 dacB b3183: obgE - GTPase involved in cell partioning and DNA repair (NCBI), at 3,328,604 to 3,329,776 obgE b3184: yhbE - conserved inner membrane protein (NCBI), at 3,329,792 to 3,330,757 yhbE
Group Condition b3180 b3181 b3182 b3183 b3184
yhbY greA dacB obgE yhbE
gfp_control GFP_control -0.9 -8.1 +0.2 -3.4 -0.6
motility outer cut, LB soft agar motility assay -2.6 -4.2 -0.1 -3.5 -0.7
sir2-hera SIR2-HerA -1.0 -6.9 +0.4 -2.9 -0.6
gfp_control GFP_control -1.4 -6.4 +0.5 -3.0 -0.5
phage T7_phage 0.00015625 MOI TC2 -0.5 -6.7 +0.0 -2.9 -0.5
stress LB Aerobic with Ramoplanin 0.000125 mM -0.8 -2.8 -3.7 -1.6 -0.6
stress LB Aerobic with Ramoplanin 0.000125 mM -1.1 -2.4 -3.9 -1.5 -0.5
mzaabcde mzaABCDE -1.0 -4.7 +0.2 -3.1 -0.5
drt_type_3 DRT_type_3 -1.0 -4.3 +0.3 -3.4 -0.6
mzaabcde mzaABCDE -1.2 -4.6 +0.2 -3.1 -0.4
mzaabcde mzaABCDE -1.2 -4.3 +0.4 -3.1 -0.6
retron_ec_48_control retron_Ec_48_control -1.3 -4.6 +0.4 -3.3 -0.2
ppi ppI -1.2 -4.2 +0.4 -3.4 -0.5
drt_type_3 DRT_type_3 -1.2 -4.2 +0.4 -3.4 -0.4
sir2-hera SIR2-HerA -1.0 -3.8 +0.3 -3.5 -0.4
drt_type_3 DRT_type_3 -0.9 -4.1 +0.4 -3.3 -0.3
ppi ppI -0.9 -3.5 +0.3 -3.4 -0.5
sir2-hera SIR2-HerA -1.0 -3.3 +0.4 -3.3 -0.5
phage T7_phage 0.00015625 MOI TC4 -1.4 -4.4 +0.4 -1.7 -0.7
phage T7_phage 0.000015625 MOI TC3 -0.2 -4.4 +0.1 -3.0 -0.2
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -0.2 -0.8 +0.1 -6.3 -0.3
ppi ppI -1.1 -3.0 +0.3 -3.4 -0.4
retron_ec_48_control retron_Ec_48_control -0.8 -3.3 +0.4 -3.3 -0.5
retron_ec_48_control retron_Ec_48_control -0.8 -3.0 +0.4 -3.4 -0.5
gfp_control GFP_control -1.3 -2.5 +0.4 -3.1 -0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +4.5 -2.7 -4.3 -1.2 -0.8
stress Benzalkonium Chloride 0.004 mg/ml -0.8 +6.4 -0.3 +0.7 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +3.5 +0.2 +3.7 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.7 +3.5 +0.2 +3.3 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.0 +4.6 +0.1 +1.9 +0.6
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