Fitness for 5 genes in Escherichia coli BW25113

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500 ntftsH and rlmE are separated by 99 nucleotidesrlmE and yhbY are separated by 125 nucleotidesyhbY and greA are separated by 155 nucleotidesgreA and dacB are separated by 247 nucleotides b3178: ftsH - protease, ATP-dependent zinc-metallo (NCBI), at 3,323,023 to 3,324,957 ftsH b3179: rlmE - 23S rRNA U2552 ribose 2'-O-methyltransferase, SAM-dependent (NCBI), at 3,325,057 to 3,325,686 rlmE b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105 yhbY b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737 greA b3182: dacB - D-alanyl-D-alanine carboxypeptidase (NCBI), at 3,326,985 to 3,328,418 dacB
Group Condition b3178 b3179 b3180 b3181 b3182
ftsH rlmE yhbY greA dacB
gfp_control GFP_control N.D. N.D. -0.9 -8.1 +0.2
sir2-hera SIR2-HerA N.D. N.D. -1.0 -6.9 +0.4
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. -0.8 -2.8 -3.7
gfp_control GFP_control N.D. N.D. -1.4 -6.4 +0.5
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. -1.1 -2.4 -3.9
phage T7_phage 0.00015625 MOI TC2 N.D. N.D. -0.5 -6.7 +0.0
motility outer cut, LB soft agar motility assay N.D. N.D. -2.6 -4.2 -0.1
phage T7_phage 0.0015625 MOI TC1 N.D. N.D. -0.5 -5.2 +0.2
mzaabcde mzaABCDE N.D. N.D. -1.2 -4.6 +0.2
mzaabcde mzaABCDE N.D. N.D. -1.0 -4.7 +0.2
retron_ec_48_control retron_Ec_48_control N.D. N.D. -1.3 -4.6 +0.4
phage T7_phage 0.00015625 MOI TC4 N.D. N.D. -1.4 -4.4 +0.4
mzaabcde mzaABCDE N.D. N.D. -1.2 -4.3 +0.4
drt_type_3 DRT_type_3 N.D. N.D. -1.0 -4.3 +0.3
ppi ppI N.D. N.D. -1.2 -4.2 +0.4
drt_type_3 DRT_type_3 N.D. N.D. -1.2 -4.2 +0.4
drt_type_3 DRT_type_3 N.D. N.D. -0.9 -4.1 +0.4
phage T7_phage 0.000015625 MOI TC3 N.D. N.D. -0.2 -4.4 +0.1
sir2-hera SIR2-HerA N.D. N.D. -1.0 -3.8 +0.3
ppi ppI N.D. N.D. -0.9 -3.5 +0.3
sir2-hera SIR2-HerA N.D. N.D. -1.0 -3.3 +0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. +4.5 -2.7 -4.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. +2.2 -2.3 +0.3
stress Cephalothin 0.01 mg/ml N.D. N.D. -1.5 +4.6 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -1.1 +3.8 -0.0
stress Aluminum chloride 5 mM N.D. N.D. +1.9 +2.7 -0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. -0.6 +5.3 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. -0.0 +5.2 -0.0
stress Benzalkonium Chloride 0.004 mg/ml N.D. N.D. -0.8 +6.4 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +1.0 +4.6 +0.1
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