Fitness for 5 genes in Escherichia coli BW25113

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500 ntsraG and rpsO are separated by 16 nucleotidesrpsO and truB are separated by 148 nucleotidestruB and rbfA overlap by 1 nucleotidesrbfA and infB are separated by 163 nucleotides b4449: sraG - unknown RNA (NCBI), at 3,309,247 to 3,309,420 sraG b3165: rpsO - 30S ribosomal protein S15 (NCBI), at 3,309,437 to 3,309,706 rpsO b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB b3167: rbfA - ribosome-binding factor A (NCBI), at 3,310,799 to 3,311,200 rbfA b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036 infB
Group Condition b4449 b3165 b3166 b3167 b3168
sraG rpsO truB rbfA infB
nitrogen source Putrescine (N) N.D. N.D. -2.8 N.D. N.D.
stress Bacitracin 1 mg/ml N.D. N.D. -2.5 N.D. N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM N.D. N.D. -2.5 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. -2.3 N.D. N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. N.D. -2.2 N.D. N.D.
stress Cephalothin 0.01 mg/ml N.D. N.D. -1.7 N.D. N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. -1.7 N.D. N.D.
phage RB68 N.D. N.D. -1.6 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. -1.6 N.D. N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM N.D. N.D. -1.6 N.D. N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. N.D. -1.5 N.D. N.D.
stress Chloramphenicol 0.001 mg/ml N.D. N.D. -1.5 N.D. N.D.
phage RB68 N.D. N.D. -1.4 N.D. N.D.
nitrogen source Putrescine (N) N.D. N.D. -1.4 N.D. N.D.
phage Ste41 N.D. N.D. -1.2 N.D. N.D.
stress decyl alcohol 5 vol% N.D. N.D. -1.1 N.D. N.D.
stress Trimethoprim 0.00125 mM N.D. N.D. +1.1 N.D. N.D.
carbon source Glycolic Acid (C) N.D. N.D. +1.3 N.D. N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. N.D. +1.3 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +1.3 N.D. N.D.
carbon source Glycolic Acid (C) N.D. N.D. +1.3 N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. +1.3 N.D. N.D.
stress Trimethoprim 0.0025 mM N.D. N.D. +1.4 N.D. N.D.
stress Benzalkonium Chloride 0.004 mg/ml N.D. N.D. +1.5 N.D. N.D.
stress Cobalt chloride 0.32 mM N.D. N.D. +1.8 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +2.0 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +2.3 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. +2.4 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +2.6 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. +3.0 N.D. N.D.
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