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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yraR and yhbO are separated by 127 nucleotides
yhbO and yhbP overlap by 21 nucleotides
yhbP and yhbQ are separated by 50 nucleotides
yhbQ and yhbS overlap by 14 nucleotides
b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868
yraR
b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514
yhbO
b3154: yhbP - hypothetical protein (NCBI), at 3,297,494 to 3,297,937
yhbP
b3155: yhbQ - hypothetical protein (NCBI), at 3,297,988 to 3,298,290
yhbQ
b3156: yhbS - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,298,277 to 3,298,780
yhbS
Group
Condition
b3152
b3153
b3154
b3155
b3156
yraR
yhbO
yhbP
yhbQ
yhbS
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
+0.1
-1.6
-0.7
-0.4
stress
Cisplatin 0.1 mg/ml
+0.1
-0.1
-0.4
-1.1
-1.0
phage
Ste41
-0.0
-0.8
-0.7
-0.4
-0.4
carbon source
Acetate (C)
-0.4
+0.0
-0.3
-0.4
-1.0
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.5
-0.3
-0.1
-1.6
-0.4
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+0.0
-0.7
+0.1
-0.6
-0.7
carbon source
Glycolic Acid (C)
-0.4
+0.3
-0.1
-0.3
-1.1
phage
Δdbn_10
-2.2
+0.7
-0.2
+0.4
-0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
+0.1
+0.4
-0.5
-0.8
motility
inner cut, LB soft agar motility assay
-0.7
+0.5
+0.1
-0.5
-0.4
phage
T7_phage 0.00015625 MOI TC4
+0.5
-0.0
-0.4
-0.6
-0.4
stress
Cephalothin 0.01 mg/ml
-0.4
-0.2
+0.9
-0.6
-0.7
phage
Ste41
-0.5
+0.1
+0.7
-1.2
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
+0.2
-0.4
+0.4
-0.7
phage
RB68
+0.7
-0.2
+0.1
-0.7
-0.5
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.6
-0.6
+0.4
-0.5
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.9
+0.4
-0.5
-1.2
+0.1
motility
outer cut, LB soft agar motility assay
+0.0
+1.3
-1.3
-0.1
-0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
+0.6
+0.6
-0.8
-0.3
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.1
+0.9
-0.3
-0.9
-0.1
phage
Phage_N4_cage_3_mice _Feces_40 hr
-0.4
+0.9
-0.3
-0.6
+0.2
carbon source
Ying_Others16 rep B; time point 2
-0.1
-0.2
+0.1
-0.7
+1.0
stress
3-methyl-1-butanol 1.25 vol%
-0.7
+0.5
+1.0
-0.2
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.9
-0.3
+0.6
-0.3
+0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.4
-1.1
+3.0
-0.3
+0.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.4
+0.7
+0.1
-0.8
+0.5
carbon source
Ying_Others16 rep A; time point 2
+0.3
-0.2
+0.2
-0.4
+1.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.1
-0.1
+0.5
+0.1
+0.4
stress
Bacitracin 1 mg/ml
+0.4
+0.6
+1.4
-0.4
+0.2
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.2
+0.7
+1.2
+0.8
-0.1
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