Fitness for 5 genes in Escherichia coli BW25113

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500 ntyraR and yhbO are separated by 127 nucleotidesyhbO and yhbP overlap by 21 nucleotidesyhbP and yhbQ are separated by 50 nucleotidesyhbQ and yhbS overlap by 14 nucleotides b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868 yraR b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514 yhbO b3154: yhbP - hypothetical protein (NCBI), at 3,297,494 to 3,297,937 yhbP b3155: yhbQ - hypothetical protein (NCBI), at 3,297,988 to 3,298,290 yhbQ b3156: yhbS - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,298,277 to 3,298,780 yhbS
Group Condition b3152 b3153 b3154 b3155 b3156
yraR yhbO yhbP yhbQ yhbS
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +0.1 -1.6 -0.7 -0.4
stress Cisplatin 0.1 mg/ml +0.1 -0.1 -0.4 -1.1 -1.0
phage Ste41 -0.0 -0.8 -0.7 -0.4 -0.4
carbon source Acetate (C) -0.4 +0.0 -0.3 -0.4 -1.0
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.5 -0.3 -0.1 -1.6 -0.4
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.0 -0.7 +0.1 -0.6 -0.7
carbon source Glycolic Acid (C) -0.4 +0.3 -0.1 -0.3 -1.1
phage Δdbn_10 -2.2 +0.7 -0.2 +0.4 -0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 +0.1 +0.4 -0.5 -0.8
motility inner cut, LB soft agar motility assay -0.7 +0.5 +0.1 -0.5 -0.4
phage T7_phage 0.00015625 MOI TC4 +0.5 -0.0 -0.4 -0.6 -0.4
stress Cephalothin 0.01 mg/ml -0.4 -0.2 +0.9 -0.6 -0.7
phage Ste41 -0.5 +0.1 +0.7 -1.2 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 +0.2 -0.4 +0.4 -0.7
phage RB68 +0.7 -0.2 +0.1 -0.7 -0.5
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.6 -0.6 +0.4 -0.5 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.9 +0.4 -0.5 -1.2 +0.1
motility outer cut, LB soft agar motility assay +0.0 +1.3 -1.3 -0.1 -0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 +0.6 +0.6 -0.8 -0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.1 +0.9 -0.3 -0.9 -0.1
phage Phage_N4_cage_3_mice _Feces_40 hr -0.4 +0.9 -0.3 -0.6 +0.2
carbon source Ying_Others16 rep B; time point 2 -0.1 -0.2 +0.1 -0.7 +1.0
stress 3-methyl-1-butanol 1.25 vol% -0.7 +0.5 +1.0 -0.2 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.9 -0.3 +0.6 -0.3 +0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.4 -1.1 +3.0 -0.3 +0.6
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.4 +0.7 +0.1 -0.8 +0.5
carbon source Ying_Others16 rep A; time point 2 +0.3 -0.2 +0.2 -0.4 +1.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.1 -0.1 +0.5 +0.1 +0.4
stress Bacitracin 1 mg/ml +0.4 +0.6 +1.4 -0.4 +0.2
stress LB Anaerobic with Ampicillin 0.00000195313 mM -0.2 +0.7 +1.2 +0.8 -0.1
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