Fitness for 5 genes in Escherichia coli BW25113

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500 ntgarR and garL are separated by 35 nucleotidesgarL and garP are separated by 15 nucleotidesgarP and garD are separated by 374 nucleotidesgarD and sohA are separated by 148 nucleotides b3125: garR - tartronate semialdehyde reductase (RefSeq), at 3,269,889 to 3,270,773 garR b3126: garL - alpha-dehydro-beta-deoxy-D-glucarate aldolase (NCBI), at 3,270,809 to 3,271,579 garL b3127: garP - predicted (D)-galactarate transporter (NCBI), at 3,271,595 to 3,272,929 garP b3128: garD - (D)-galactarate dehydrogenase (NCBI), at 3,273,304 to 3,274,875 garD b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359 sohA
Group Condition b3125 b3126 b3127 b3128 b3129
garR garL garP garD sohA
carbon source Glycolic Acid (C) -1.3 -1.0 -2.2 +0.2 -0.5
carbon source Glycolic Acid (C) -0.8 -0.4 -2.0 -0.1 -0.5
carbon source carbon source D-Glucose 0.25mM -0.8 -0.8 -0.7 +0.3 -1.0
phage T7_phage 0.0015625 MOI TC2 -0.2 +0.4 -0.8 -0.2 -2.1
carbon source carbon source D-Mannose 5mM -0.2 -0.4 -0.6 +0.1 -1.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -0.1 +0.0 -0.2 -1.8
phage Ste41 -0.5 -0.0 +0.5 -0.0 -1.7
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.0 -0.1 +0.1 -0.1 -1.5
stress LB Aerobic with Ramoplanin 0.000125 mM +0.6 -0.2 -0.3 +0.1 -1.2
stress Aluminum chloride 5 mM -0.2 -1.3 -0.3 +0.1 +0.8
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 +0.2 +0.2 -0.2 -1.4
stress Cisplatin 0.1 mg/ml +0.2 -1.1 -0.2 +0.3 +0.3
stress 3-methyl-1-butanol 1.25 vol% +0.1 +0.4 +0.4 -0.2 -1.1
stress 3-methyl-1-butanol 1.25 vol% +0.3 +0.4 +0.2 +0.3 -1.5
stress LB Anaerobic with Trimethoprim 0.0000003125 mM +0.4 +0.3 -0.1 +0.2 -1.0
stress Chloramphenicol 0.005 mg/ml +0.3 -0.6 -0.4 -0.1 +0.6
stress Chlorite 1.25 mM +0.2 -0.6 -0.1 +0.3 +0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 +0.9 -0.1 +0.2 -1.0
phage Ste41 +0.4 -0.6 +0.6 +0.1 +0.2
stress Trimethoprim 0.00125 mM -0.2 -0.1 -0.0 -0.0 +1.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 +0.1 -0.4 -0.3 +1.9
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.0 +0.0 -0.3 +0.3 +1.4
carbon source carbon source D-Glucose 5mM +0.1 +0.2 -0.2 +0.4 +1.0
motility inner cut, LB soft agar motility assay +0.3 +0.2 +0.4 -0.1 +0.9
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.0 -0.0 +0.3 +0.2 +1.4
stress Trimethoprim 0.0025 mM +0.3 -0.0 -0.1 -0.2 +1.9
stress Cephalothin 0.01 mg/ml -0.1 -0.3 +0.2 +0.1 +2.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.4 -0.2 +0.1 +1.5
control PBS_cage_1_mice _Feces_16 hr +0.4 +0.9 +0.4 +0.1 +0.6
motility outer cut, LB soft agar motility assay +0.7 +0.8 +0.4 +0.0 +0.7
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