Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhaC and rnpB are separated by 613 nucleotidesrnpB and garK are separated by 32 nucleotidesgarK and garR are separated by 96 nucleotidesgarR and garL are separated by 35 nucleotides b3121: yhaC - hypothetical protein (NCBI), at 3,266,437 to 3,267,624 yhaC b3123: rnpB - RNase P RNA component precursor RnpB (NCBI), at 3,268,238 to 3,268,614 rnpB b3124: garK - glycerate kinase I (RefSeq), at 3,268,647 to 3,269,792 garK b3125: garR - tartronate semialdehyde reductase (RefSeq), at 3,269,889 to 3,270,773 garR b3126: garL - alpha-dehydro-beta-deoxy-D-glucarate aldolase (NCBI), at 3,270,809 to 3,271,579 garL
Group Condition b3121 b3123 b3124 b3125 b3126
yhaC rnpB garK garR garL
carbon source Glycolic Acid (C) -2.2 N.D. -4.1 -1.3 -1.0
carbon source Glycolic Acid (C) -0.8 N.D. -4.5 -0.8 -0.4
carbon source D-Galacturonic Acid (C) -2.0 N.D. -3.5 -0.6 -0.3
carbon source D-Glucuronic Acid (C) -1.0 N.D. -4.9 -0.4 +0.0
carbon source D-Galacturonic Acid (C) -1.8 N.D. -3.4 -0.6 -0.2
carbon source D-Glucuronic Acid (C) -0.8 N.D. -3.8 -0.5 -0.2
carbon source D-Galacturonic Acid (C) M9 -0.6 N.D. -3.6 -0.4 -0.6
carbon source D-Galacturonic Acid (C) MOPS -0.4 N.D. -3.3 -0.5 -0.4
motility inner cut, LB soft agar motility assay -4.3 N.D. -0.3 +0.3 +0.2
motility inner cut, LB soft agar motility assay -3.7 N.D. -0.5 +0.4 +0.0
carbon source D-Ribose (C) -2.1 N.D. -1.7 -0.1 +0.0
motility outer cut, LB soft agar motility assay -3.4 N.D. +0.0 -0.6 +0.3
carbon source D-Serine (C) -0.4 N.D. -3.0 -0.1 +0.1
motility inner cut, LB soft agar motility assay -2.8 N.D. -0.1 +0.0 -0.3
protonated/deuterated protonated glucose in D2O -2.4 N.D. -0.6 -0.1 -0.0
stress Cisplatin 0.05 mg/ml -2.7 N.D. +0.0 -0.1 -0.2
stress Cisplatin 0.1 mg/ml -2.2 N.D. +0.1 +0.2 -1.1
M9 Glucose 2% aerobic -2.4 N.D. -0.2 -0.1 -0.2
motility inner cut, LB soft agar motility assay -3.1 N.D. -0.2 +0.3 +0.1
nitrogen source L-Alanine (N) -2.4 N.D. -0.0 -0.3 -0.2
carbon source D-Ribose (C) -1.2 N.D. -1.6 +0.0 -0.0
nitrogen source L-Alanine (N) -2.6 N.D. +0.2 +0.1 -0.1
carbon source D-Serine (C) +0.3 N.D. -2.5 -0.3 +0.0
stress 2,4-Dinitrophenol 2 mM +0.3 N.D. -2.3 -0.0 -0.2
motility outer cut, LB soft agar motility assay -2.7 N.D. -0.5 +0.7 +0.8
control PBS_cage_1_mouse_1_Cecum_40 hr +1.3 N.D. -1.4 -0.4 +0.7
phage T7_phage 0.0015625 MOI TC2 +2.0 N.D. -0.5 -0.2 +0.4
phage N4_phage 0.009375 MOI TC2 +2.8 N.D. -0.3 -0.0 -0.4
control PBS_cage_1_mice _Feces_16 hr +1.6 N.D. -0.6 +0.4 +0.9
phage Papa dilution 10-1 cacl2 maltose +3.3 N.D. +0.7 +0.1 +0.0
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