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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
exuT and exuR are separated by 129 nucleotides
exuR and yqjA are separated by 344 nucleotides
yqjA and yqjB are separated by 3 nucleotides
yqjB and yqjC are separated by 146 nucleotides
b3093: exuT - hexuronate transporter (NCBI), at 3,243,126 to 3,244,544
exuT
b3094: exuR - negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS), at 3,244,674 to 3,245,450
exuR
b3095: yqjA - conserved inner membrane protein (NCBI), at 3,245,795 to 3,246,457
yqjA
b3096: yqjB - hypothetical protein (NCBI), at 3,246,461 to 3,246,844
yqjB
b3097: yqjC - orf, hypothetical protein (VIMSS), at 3,246,991 to 3,247,359
yqjC
Group
Condition
b3093
b3094
b3095
b3096
b3097
exuT
exuR
yqjA
yqjB
yqjC
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.3
-0.7
-3.5
-0.9
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
-0.7
-4.5
-0.3
+0.2
stress
3-methyl-1-butanol 0.625 vol%
-0.1
-0.6
-3.4
-0.6
-0.5
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.1
-0.7
-3.9
+0.1
-0.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.1
-0.9
-3.5
-0.2
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.1
-4.9
+1.4
-0.9
-0.1
stress
LB + carbenicillin regrowth after 48hr
-0.1
-0.7
-1.1
-1.8
-0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
-0.2
-0.4
-2.9
-0.2
carbon source
D-Galacturonic Acid (C) M9
-2.6
-1.7
+0.5
+0.0
-0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.4
-1.7
+0.5
-3.1
-0.0
stress
3-methyl-1-butanol 0.625 vol%
-0.0
-0.4
-2.9
-0.4
-0.2
stress
LB + carbenicillin regrowth after 48hr
-0.1
-0.4
-1.3
-1.9
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
-4.4
+1.2
-0.4
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.1
-2.1
+0.5
-1.8
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-3.5
+0.2
+0.3
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
-3.9
+1.2
-0.4
+0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
-4.0
+1.6
-0.4
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.2
-0.9
+0.9
-2.2
-0.2
stress
Bacitracin 1 mg/ml
+0.1
-0.6
+0.8
-1.7
-0.6
carbon source
D-Galacturonic Acid (C) MOPS
-1.5
-0.9
+0.6
-0.2
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-2.8
+1.3
-0.3
-0.1
phage
RB68
+0.9
+0.5
-0.8
-1.3
-0.7
stress
Cephalothin 0.01 mg/ml
-0.1
-0.7
+1.9
-1.3
-0.9
phage
RB68
+1.0
+0.4
-0.5
-1.6
-0.2
stress
Carbenicillin 0.008 mg/ml
+0.1
-0.2
+2.0
-0.9
-0.9
motility
outer cut, LB soft agar motility assay
+0.3
-1.1
+0.2
+1.5
-0.6
motility
outer cut, LB soft agar motility assay
+0.1
-1.2
+1.1
+2.3
-0.6
motility
outer cut, LB soft agar motility assay
+0.3
-1.1
+0.5
+1.5
+0.7
phage
phage
-0.1
-0.5
+1.1
+2.5
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
-1.0
+3.7
+0.2
+0.4
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