Fitness for 5 genes in Escherichia coli BW25113

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500 ntuxaC and exuT are separated by 362 nucleotidesexuT and exuR are separated by 129 nucleotidesexuR and yqjA are separated by 344 nucleotidesyqjA and yqjB are separated by 3 nucleotides b3092: uxaC - glucuronate isomerase (NCBI), at 3,241,351 to 3,242,763 uxaC b3093: exuT - hexuronate transporter (NCBI), at 3,243,126 to 3,244,544 exuT b3094: exuR - negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS), at 3,244,674 to 3,245,450 exuR b3095: yqjA - conserved inner membrane protein (NCBI), at 3,245,795 to 3,246,457 yqjA b3096: yqjB - hypothetical protein (NCBI), at 3,246,461 to 3,246,844 yqjB
Group Condition b3092 b3093 b3094 b3095 b3096
uxaC exuT exuR yqjA yqjB
carbon source D-Galacturonic Acid (C) M9 -4.3 -2.6 -1.7 +0.5 +0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.3 +0.0 -0.7 -4.5 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.3 -0.3 -0.7 -3.5 -0.9
carbon source D-Galacturonic Acid (C) MOPS -3.4 -1.5 -0.9 +0.6 -0.2
stress 3-methyl-1-butanol 0.625 vol% -0.2 -0.1 -0.6 -3.4 -0.6
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -0.1 -0.7 -3.9 +0.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.0 -0.1 -0.9 -3.5 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -0.1 -4.9 +1.4 -0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 +0.4 -1.7 +0.5 -3.1
stress LB + carbenicillin regrowth after 48hr -0.0 -0.1 -0.7 -1.1 -1.8
stress 3-methyl-1-butanol 0.625 vol% -0.1 -0.0 -0.4 -2.9 -0.4
stress LB + carbenicillin regrowth after 48hr -0.0 -0.1 -0.4 -1.3 -1.9
carbon source D-Galacturonic Acid (C) -3.4 -1.1 +0.6 +0.4 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 -0.1 -0.2 -0.4 -2.9
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 +0.1 -2.1 +0.5 -1.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -0.4 -3.5 +0.2 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.0 -4.4 +1.2 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 -0.2 -4.0 +1.6 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 +0.0 -3.9 +1.2 -0.4
carbon source D-Galacturonic Acid (C) -3.1 -1.1 +0.7 +0.4 +0.1
carbon source D-Glucuronic Acid (C) -1.7 -1.4 +0.1 +0.2 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 +0.2 -0.9 +0.9 -2.2
carbon source D-Glucuronic Acid (C) -1.6 -1.2 +0.7 +0.3 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 +0.2 -2.8 +1.3 -0.3
phage RB68 +0.1 +0.9 +0.5 -0.8 -1.3
stress Cephalothin 0.01 mg/ml -0.1 -0.1 -0.7 +1.9 -1.3
motility outer cut, LB soft agar motility assay -0.1 +0.3 -1.1 +0.5 +1.5
motility outer cut, LB soft agar motility assay +0.2 +0.1 -1.2 +1.1 +2.3
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +0.1 -1.0 +3.7 +0.2
phage phage +0.2 -0.1 -0.5 +1.1 +2.5
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