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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
sstT and ygjV are separated by 4 nucleotides
ygjV and uxaA are separated by 82 nucleotides
uxaA and uxaC are separated by 14 nucleotides
uxaC and exuT are separated by 362 nucleotides
b3089: sstT - sodium:serine/threonine symporter (NCBI), at 3,237,966 to 3,239,210
sstT
b3090: ygjV - conserved inner membrane protein (NCBI), at 3,239,215 to 3,239,766
ygjV
b3091: uxaA - altronate hydrolase (NCBI), at 3,239,849 to 3,241,336
uxaA
b3092: uxaC - glucuronate isomerase (NCBI), at 3,241,351 to 3,242,763
uxaC
b3093: exuT - hexuronate transporter (NCBI), at 3,243,126 to 3,244,544
exuT
Group
Condition
b3089
b3090
b3091
b3092
b3093
sstT
ygjV
uxaA
uxaC
exuT
carbon source
D-Galacturonic Acid (C) M9
-0.0
-0.1
-4.5
-4.3
-2.6
carbon source
D-Galacturonic Acid (C)
-0.0
-0.2
-5.0
-3.4
-1.1
carbon source
D-Galacturonic Acid (C)
-0.0
-0.1
-5.1
-3.1
-1.1
carbon source
D-Galacturonic Acid (C) MOPS
-0.0
-0.0
-4.5
-3.4
-1.5
carbon source
D-Glucuronic Acid (C)
+0.0
+0.0
-0.1
-1.7
-1.4
carbon source
D-Glucuronic Acid (C)
+0.0
-0.0
-0.1
-1.6
-1.2
carbon source
D-Galacturonic Acid (C) MOPS
-0.0
+0.1
-1.9
-0.9
-0.1
carbon source
D-Galacturonic Acid (C) MOPS
-0.1
+0.1
-1.9
-0.5
-0.2
nophagecontrol
R1_N4_T1_phage0
-0.2
-0.5
-0.2
-0.0
-0.3
stress
octanol 0.08 vol%
+0.0
-0.4
-0.2
+0.1
-0.6
control
PBS_cage_2_mouse_1_Cecum_40 hr
-0.1
-0.6
-0.3
-0.2
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.1
-0.2
-0.1
-0.3
-0.3
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.1
-0.3
-0.2
-0.1
-0.4
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.5
-0.2
-0.1
-0.0
control
PBS_cage_1_mice _Feces_40 hr
+0.1
-0.7
-0.1
+0.2
-0.2
phage
Δdbn_10
-0.3
-0.3
-0.3
+0.2
+0.2
control
PBS_cage_1_mice _Feces_22 hr
+0.1
-0.4
-0.1
+0.3
-0.1
phage
Ste41
-0.5
-0.1
-0.0
-0.0
+0.6
motility
inner cut, LB soft agar motility assay
-0.2
-0.3
+0.2
+0.1
+0.2
phage
R2_N4_T1_phage10
-0.2
+0.6
-0.2
-0.2
+0.0
control
PBS_cage_1_mice _Feces_16 hr
+0.3
-0.2
+0.1
+0.3
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.4
+0.0
-0.1
-0.4
+0.4
motility
outer cut, LB soft agar motility assay
+0.4
+0.1
-0.2
-0.1
+0.3
phage
Phage_N4_cage_3_mouse_2_Cecum_40 hr
+0.0
+0.3
-0.2
+0.1
+0.3
carbon source
Acetate (C)
+0.2
+0.1
-0.2
+0.4
+0.1
phage
RB68
+0.2
-0.2
-0.1
-0.0
+1.0
phage
RB68
+0.0
+0.1
-0.3
+0.1
+0.9
phage
T7_phage 0.0015625 MOI TC1
+0.2
+0.0
+0.3
+0.4
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.2
+0.6
-0.0
+0.2
+0.3
phage
Ste41
+0.4
+0.2
+0.0
+0.5
+0.7
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