Fitness for 5 genes in Escherichia coli BW25113

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500 ntyqjH and yqjI are separated by 287 nucleotidesyqjI and aer are separated by 153 nucleotidesaer and ygjG are separated by 417 nucleotidesygjG and ygjH are separated by 41 nucleotides b3070: yqjH - predicted siderophore interacting protein (NCBI), at 3,213,749 to 3,214,513 yqjH b3071: yqjI - predicted transcriptional regulator (NCBI), at 3,214,801 to 3,215,424 yqjI b3072: aer - fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component (NCBI), at 3,215,578 to 3,217,098 aer b3073: ygjG - putrescine:2-oxoglutaric acid aminotransferase, PLP-dependent (RefSeq), at 3,217,516 to 3,218,895 ygjG b3074: ygjH - hypothetical protein (NCBI), at 3,218,937 to 3,219,269 ygjH
Group Condition b3070 b3071 b3072 b3073 b3074
yqjH yqjI aer ygjG ygjH
phage T7_phage 0.0015625 MOI TC2 -0.2 -2.6 +0.2 -0.7 -2.0
phage HSDP4 -0.7 -1.0 -0.8 -0.2 -0.9
phage T7_phage 0.00015625 MOI TC2 -0.5 -1.9 -0.1 +0.0 -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr -0.7 -0.6 -0.6 -0.1 -0.5
stress methylglyoxal 0.008 vol% -0.3 -1.5 -0.3 -0.2 -0.2
nitrogen source Putrescine (N) -0.5 -0.3 -0.1 -1.4 -0.0
motility outer cut, LB soft agar motility assay -0.9 -0.9 -0.5 +0.2 +0.1
phage T7_phage 0.00015625 MOI TC4 -0.4 -1.8 -0.0 +0.5 -0.3
carbon source carbon source D-Mannose 1mM +0.3 -1.8 -0.3 -0.1 -0.0
stress .5% DMSO +0.3 -1.4 -0.1 -0.2 -0.4
phage Δdbn_10 +0.2 -1.5 +0.1 -0.2 -0.2
phage T7_phage 0.0015625 MOI TC1 -0.5 -1.4 -0.1 +0.4 -0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.2 +0.3 -0.4 +0.1 -1.1
stress benzenesulfonyl fluoride +0.4 -1.0 -0.4 -0.0 +0.2
phage TP4 -0.5 -1.0 +0.5 -0.0 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM +0.3 +1.0 +0.2 -0.0 -0.7
stress octanol 0.08 vol% +0.8 +0.2 +0.5 +0.2 -0.3
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 +0.4 -0.1 -0.3 +1.0
carbon source carbon source D-Glucose 5mM +0.4 +1.0 -0.2 -0.2 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +1.6 -0.3 -0.3 +0.4
stress 3-methyl-1-butanol 1.25 vol% +0.5 +0.2 +0.5 -0.0 +0.8
phage Ste41 +0.6 +0.8 -0.1 +0.3 +0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.5 +2.3 -0.0 -0.3 -0.2
stress Trimethoprim 0.00125 mM +0.5 +1.1 +0.2 +0.3 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.7 +1.7 +0.3 -0.1 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.1 +1.5 +0.2 +0.1 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +2.1 -0.1 +0.6 +0.2
phage Papa dilution 10-1 cacl2 maltose +1.0 +1.1 +0.8 +0.3 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.7 +2.2 +0.0 -0.2 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.9 +2.6 -0.0 +0.3 +0.9
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