Fitness for 5 genes in Escherichia coli BW25113

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500 ntrfaE and glnE are separated by 47 nucleotidesglnE and ygiF are separated by 22 nucleotidesygiF and htrG are separated by 241 nucleotideshtrG and cca are separated by 63 nucleotides b3052: rfaE - fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI), at 3,193,342 to 3,194,775 rfaE b3053: glnE - fused deadenylyltransferase/adenylyltransferase for glutamine synthetase (NCBI), at 3,194,823 to 3,197,663 glnE b3054: ygiF - predicted adenylate cyclase (NCBI), at 3,197,686 to 3,198,987 ygiF b3055: htrG - predicted signal transduction protein (SH3 domain) (NCBI), at 3,199,229 to 3,199,849 htrG b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151 cca
Group Condition b3052 b3053 b3054 b3055 b3056
rfaE glnE ygiF htrG cca
carbon source Ying_sugar7 rep B; time point 3 -5.8 -2.4 -0.0 -0.2 N.D.
carbon source Ying_sugar7 rep C; time point 3 -5.9 -2.3 -0.1 -0.1 N.D.
carbon source Ying_sugar7 rep C; time point 2 -6.3 -1.8 -0.0 -0.2 N.D.
carbon source Ying_sugar7 rep A; time point 3 -5.4 -2.3 +0.0 -0.3 N.D.
phage Phage_N4_cage_3_mice _Feces_22 hr -7.5 -0.1 +0.0 -0.1 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -7.1 +0.0 -0.3 -0.3 N.D.
phage Phage_N4_cage_4_mice _Feces_22 hr -7.5 -0.0 -0.0 +0.0 N.D.
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -6.9 -0.2 +0.1 -0.4 N.D.
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr -7.4 +0.0 +0.2 -0.2 N.D.
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -7.5 +0.3 -0.1 -0.0 N.D.
carbon source Ying_sugar7 rep B; time point 2 -5.7 -1.5 +0.0 -0.2 N.D.
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -7.3 -0.0 -0.0 +0.0 N.D.
phage Phage_N4_cage_3_mice _Feces_16 hr -7.0 -0.1 -0.0 -0.2 N.D.
carbon source Ying_sugar7 rep A; time point 2 -5.2 -1.6 -0.2 -0.3 N.D.
control PBS_cage_2_mice _Feces_40 hr -7.0 -0.2 +0.2 -0.0 N.D.
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr -7.2 -0.1 -0.0 +0.4 N.D.
phage Phage_N4_cage_4_mice _Feces_40 hr -7.0 -0.1 +0.2 -0.0 N.D.
phage Phage_N4_cage_4_mice _Feces_16 hr -6.9 +0.1 -0.1 -0.1 N.D.
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr -7.0 -0.1 +0.2 +0.1 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 -5.4 -1.1 +0.0 -0.4 N.D.
carbon source Ying_sugar7 rep B; time point 1 -5.9 -0.7 -0.0 -0.1 N.D.
control PBS_cage_2_mice _Feces_16 hr -7.3 -0.0 +0.3 +0.2 N.D.
control PBS_cage_2_mice _Feces_22 hr -7.1 -0.0 +0.3 +0.2 N.D.
control PBS_cage_2_mouse_1_Cecum_40 hr -6.8 -0.0 +0.2 +0.1 N.D.
control PBS_cage_2_mouse_2_Cecum_40 hr -6.8 +0.1 +0.4 -0.0 N.D.
control PBS_cage_1_mice _Feces_22 hr -6.8 +0.2 +0.2 +0.4 N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr -6.1 +0.6 -0.0 +0.1 N.D.
phage RB68 +5.9 +0.2 +0.9 -0.2 N.D.
phage Δdbn_10 +8.9 -0.1 -0.2 -0.4 N.D.
phage Bas60 +9.3 +0.0 +0.1 +0.0 N.D.
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