Fitness for 5 genes in Escherichia coli BW25113

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500 ntglcF and glcE are separated by 10 nucleotidesglcE and glcD overlap by 1 nucleotidesglcD and glcC are separated by 250 nucleotidesglcC and yghO are separated by 6 nucleotides b4467: glcF - glycolate oxidase iron-sulfur subunit (NCBI), at 3,122,258 to 3,123,481 glcF b4468: glcE - glycolate oxidase FAD binding subunit (NCBI), at 3,123,492 to 3,124,544 glcE b2979: glcD - glycolate dehydrogenase, putative FAD-linked subunit (from data), at 3,124,544 to 3,126,043 glcD b2980: glcC - DNA-binding transcriptional dual regulator, glycolate-binding (NCBI), at 3,126,294 to 3,127,058 glcC b2981: yghO - predicted DNA-binding transcriptional regulator (RefSeq), at 3,127,065 to 3,128,165 yghO
Group Condition b4467 b4468 b2979 b2980 b2981
glcF glcE glcD glcC yghO
carbon source Glycolic Acid (C) -3.6 -4.4 -4.3 -3.8 +0.0
carbon source Glycolic Acid (C) -3.1 -3.1 -2.7 -4.3 -0.1
phage T7_phage 0.00015625 MOI TC3 -0.2 -1.2 -0.7 -0.4 -0.1
phage Ste41 -0.9 -0.8 -0.5 -0.1 +0.0
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.0 -0.4 -1.1 -0.3 -0.1
stress Chloramphenicol 0.005 mg/ml -0.3 -0.3 +0.1 -0.9 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 -0.6 +0.1 -0.3 -0.3
carbon source D-Galacturonic Acid (C) MOPS -0.5 -0.4 -0.3 -0.5 +0.1
stress Cephalothin 0.01 mg/ml -0.5 -0.4 -0.7 -0.3 +0.3
phage T7_phage 0.0015625 MOI TC1 -0.1 -0.9 +0.0 -0.4 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.3 +0.2 -0.8 -0.4 -0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 -0.8 +0.1 -0.1 -0.4
stress octanol 0.08 vol% -0.3 +0.3 -1.4 +0.0 +0.4
stress Cisplatin 0.1 mg/ml -0.2 -0.2 -0.2 -0.7 +0.5
phage Ste41 -0.1 -0.2 -0.7 +0.9 -0.3
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.2 -0.6 -0.2 +0.5 -0.1
stress Chlorite 1.25 mM -0.5 +0.3 +0.3 -0.5 +0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.5 +0.4 -0.6 -0.4 -0.0
mzaabcde mzaABCDE -0.4 +0.6 +0.2 -0.1 -0.3
carbon source carbon source D-Mannose 20mM +0.3 +0.7 -0.2 -0.3 -0.1
stress LB Aerobic with Irgasan 0.0000003125 mM -0.0 +0.4 +0.3 -0.5 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 +0.4 +0.1 -0.4 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.4 +0.3 +0.4 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.4 +0.3 +0.4 +0.2 +0.2
stress Benzalkonium Chloride 0.004 mg/ml -0.3 +0.8 +0.6 -0.2 +0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 -0.2 +0.6 +0.4 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 +0.4 +0.8 +0.2 -0.0
phage Papa dilution 10-1 cacl2 maltose +0.7 +0.7 +0.5 +0.2 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.6 +0.6 +0.2 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.9 +0.3 +0.5 +0.5
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