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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
glcF and glcE are separated by 10 nucleotides
glcE and glcD overlap by 1 nucleotides
glcD and glcC are separated by 250 nucleotides
glcC and yghO are separated by 6 nucleotides
b4467: glcF - glycolate oxidase iron-sulfur subunit (NCBI), at 3,122,258 to 3,123,481
glcF
b4468: glcE - glycolate oxidase FAD binding subunit (NCBI), at 3,123,492 to 3,124,544
glcE
b2979: glcD - glycolate dehydrogenase, putative FAD-linked subunit
(from data)
, at 3,124,544 to 3,126,043
glcD
b2980: glcC - DNA-binding transcriptional dual regulator, glycolate-binding (NCBI), at 3,126,294 to 3,127,058
glcC
b2981: yghO - predicted DNA-binding transcriptional regulator (RefSeq), at 3,127,065 to 3,128,165
yghO
Group
Condition
b4467
b4468
b2979
b2980
b2981
glcF
glcE
glcD
glcC
yghO
carbon source
Glycolic Acid (C)
-3.6
-4.4
-4.3
-3.8
+0.0
carbon source
Glycolic Acid (C)
-3.1
-3.1
-2.7
-4.3
-0.1
phage
T7_phage 0.00015625 MOI TC3
-0.2
-1.2
-0.7
-0.4
-0.1
phage
Ste41
-0.9
-0.8
-0.5
-0.1
+0.0
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.0
-0.4
-1.1
-0.3
-0.1
stress
Chloramphenicol 0.005 mg/ml
-0.3
-0.3
+0.1
-0.9
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.5
-0.6
+0.1
-0.3
-0.3
carbon source
D-Galacturonic Acid (C) MOPS
-0.5
-0.4
-0.3
-0.5
+0.1
stress
Cephalothin 0.01 mg/ml
-0.5
-0.4
-0.7
-0.3
+0.3
phage
T7_phage 0.0015625 MOI TC1
-0.1
-0.9
+0.0
-0.4
-0.2
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.3
+0.2
-0.8
-0.4
-0.1
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.2
-0.8
+0.1
-0.1
-0.4
stress
octanol 0.08 vol%
-0.3
+0.3
-1.4
+0.0
+0.4
stress
Cisplatin 0.1 mg/ml
-0.2
-0.2
-0.2
-0.7
+0.5
phage
Ste41
-0.1
-0.2
-0.7
+0.9
-0.3
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.2
-0.6
-0.2
+0.5
-0.1
stress
Chlorite 1.25 mM
-0.5
+0.3
+0.3
-0.5
+0.3
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.5
+0.4
-0.6
-0.4
-0.0
mzaabcde
mzaABCDE
-0.4
+0.6
+0.2
-0.1
-0.3
carbon source
carbon source D-Mannose 20mM
+0.3
+0.7
-0.2
-0.3
-0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.0
+0.4
+0.3
-0.5
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.7
+0.4
+0.1
-0.4
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.4
+0.3
+0.4
+0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.4
+0.3
+0.4
+0.2
+0.2
stress
Benzalkonium Chloride 0.004 mg/ml
-0.3
+0.8
+0.6
-0.2
+0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.4
-0.2
+0.6
+0.4
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.5
+0.4
+0.8
+0.2
-0.0
phage
Papa dilution 10-1 cacl2 maltose
+0.7
+0.7
+0.5
+0.2
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.6
+0.6
+0.2
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
+0.9
+0.3
+0.5
+0.5
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