Fitness for 5 genes in Escherichia coli BW25113

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500 ntyghJ and yghK are separated by 484 nucleotidesyghK and glcB are separated by 354 nucleotidesglcB and glcG are separated by 21 nucleotidesglcG and glcF are separated by 4 nucleotides b4466: yghJ - predicted inner membrane lipoprotein (NCBI), at 3,112,572 to 3,117,134 yghJ b2975: yghK - glycolate transporter (NCBI), at 3,117,619 to 3,119,301 yghK b2976: glcB - malate synthase (NCBI), at 3,119,656 to 3,121,827 glcB b2977: glcG - hypothetical protein (NCBI), at 3,121,849 to 3,122,253 glcG b4467: glcF - glycolate oxidase iron-sulfur subunit (NCBI), at 3,122,258 to 3,123,481 glcF
Group Condition b4466 b2975 b2976 b2977 b4467
yghJ yghK glcB glcG glcF
phage T7_phage 0.00015625 MOI TC3 -0.5 -0.1 -0.1 -1.3 -0.2
phage Ste41 -0.1 -0.3 -0.1 -0.5 -0.9
phage T7_phage 0.0015625 MOI TC2 -0.2 -0.1 +0.2 -1.2 -0.6
stress Chlorite 1.25 mM -0.1 -0.2 +0.2 -1.0 -0.5
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.2 +0.0 +0.1 -0.9 -0.6
stress Benzalkonium Chloride 0.004 mg/ml -0.0 +0.3 -0.4 -0.8 -0.3
phage Δdbn_10 -0.2 +0.1 -0.1 -0.7 -0.3
stress Cephalothin 0.01 mg/ml +0.1 -0.0 +0.1 -0.7 -0.5
stress Chlorite 0.4 mM -0.1 -0.1 +0.2 -0.6 -0.2
phage T7_phage 0.00015625 MOI TC4 -0.2 -0.4 -0.2 +0.3 -0.1
phage N4_phage 0.00009375 MOI TC4 +0.1 +0.3 +0.2 -0.6 -0.1
nophagecontrol R1_N4_T1_phage0 -0.0 -0.2 +0.2 -0.5 +0.4
stress toluene 2.5 vol% +0.0 -0.1 -0.1 +0.7 -0.6
carbon source carbon source D-Glucose 0.25mM -0.1 +0.0 -0.1 +0.7 -0.4
stress methylglyoxal 0.008 vol% +0.0 +0.2 -0.2 +0.6 -0.3
stress Aluminum chloride 5 mM -0.1 -0.0 -0.4 +0.6 +0.2
control PBS_cage_1_mice _Feces_22 hr -0.1 +0.1 +0.8 +0.3 -0.4
stress copper (II) chloride 2 mM +0.1 +0.1 -0.2 +0.7 +0.1
control PBS_cage_1_mice _Feces_40 hr -0.1 +0.1 +0.6 +0.4 -0.2
carbon source Glycolic Acid (C) +0.1 -0.6 +2.1 +2.4 -3.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.4 +0.4 +0.2 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.2 +0.4 +0.7 +0.3
carbon source Glycolic Acid (C) +0.1 -0.5 +2.5 +2.6 -3.6
motility outer cut, LB soft agar motility assay +0.1 +0.2 -0.1 +0.7 +0.2
control PBS_cage_1_mice _Feces_16 hr +0.0 +0.0 +0.8 +0.4 -0.1
carbon source carbon source D-Mannose 1mM -0.0 +0.2 -0.1 +0.6 +0.5
stress Bacitracin 1 mg/ml +0.0 +0.2 +0.1 +1.1 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -0.2 +0.4 +1.0 +0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.0 -0.1 +0.0 +1.6 +0.1
phage Papa dilution 10-1 cacl2 maltose -0.1 +0.1 +0.3 +0.8 +0.7
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