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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yggX and mltC are separated by 64 nucleotides
mltC and nupG are separated by 201 nucleotides
nupG and speC are separated by 49 nucleotides
speC and yqgA are separated by 397 nucleotides
b2962: yggX - hypothetical protein (NCBI), at 3,102,115 to 3,102,390
yggX
b2963: mltC - membrane-bound lytic murein transglycosylase C (VIMSS), at 3,102,455 to 3,103,534
mltC
b2964: nupG - transport of nucleosides, permease protein (VIMSS), at 3,103,736 to 3,104,992
nupG
b2965: speC - ornithine decarboxylase isozyme (VIMSS), at 3,105,042 to 3,107,177
speC
b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282
yqgA
Group
Condition
b2962
b2963
b2964
b2965
b2966
yggX
mltC
nupG
speC
yqgA
carbon source
Ying_Nucleobase14 rep C; time point 3
+0.3
+0.1
-3.6
-0.3
-0.1
carbon source
Ying_Nucleobase14 rep B; time point 3
+0.2
+0.1
-3.6
-0.2
+0.2
carbon source
Ying_Nucleobase14 rep A; time point 3
+0.6
+0.1
-3.5
-0.4
-0.1
phage
T7_phage 0.00015625 MOI TC3
-2.3
-0.2
-0.4
-0.2
+0.4
carbon source
Ying_Nucleobase14 rep A; time point 2
+0.5
+0.1
-2.9
-0.1
-0.0
phage
T7_phage 0.00015625 MOI TC4
-1.7
+0.0
-0.6
+0.2
-0.2
motility
outer cut, LB soft agar motility assay
-1.3
-0.1
-0.1
-0.3
-0.3
carbon source
Ying_Nucleobase14 rep B; time point 2
+0.3
+0.2
-2.5
+0.0
+0.0
carbon source
Ying_Nucleobase14 rep C; time point 2
+0.5
+0.1
-2.4
-0.0
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
+0.3
-1.8
+0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.2
+0.1
-0.5
+0.2
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.2
-1.3
+0.1
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.8
-0.2
-1.9
+0.3
-0.3
protonated/deuterated
deuterated glucose in H2O
-1.6
-0.0
+0.3
-0.0
+0.2
stress
Cephalothin 0.01 mg/ml
-1.5
+0.9
+0.2
-0.1
-0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.2
-1.0
-0.5
-0.1
+0.8
stress
octanol 0.08 vol%
-0.8
+0.7
+0.1
+0.3
-1.1
carbon source
Ying_Nucleobase14 rep B; time point 1
+0.5
-0.0
-1.2
+0.1
-0.2
carbon source
Guanosine 0.35306 mM (C)
+0.4
+0.1
-1.2
+0.3
-0.3
carbon source
Uridine 0.4095 mM (C)
+0.2
-0.0
-1.4
+0.8
-0.2
gfp_control
GFP_control
-0.6
+0.2
+0.6
-0.1
-0.7
carbon source
Uridine 0.4095 mM (C)
+0.2
-0.0
-1.1
+0.7
-0.1
stress
Trimethoprim 0.00125 mM
+0.9
+0.1
-0.7
-0.1
-0.2
carbon source
Uridine 0.4095 mM (C)
-0.0
+0.1
-1.0
+0.9
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.8
-0.7
+0.1
-0.2
+0.4
stress
3-methyl-1-butanol 1.25 vol%
+1.0
-0.3
+0.2
+0.3
+0.6
nophagecontrol
R1_N4_T1_phage0
+0.6
+0.2
-0.1
+0.2
+1.0
stress
2-Furfuraldehyde 0.25 vol%
+2.0
-0.1
-0.2
-0.1
+0.4
stress
Spectinomycin 0.0125 mg/ml
+0.3
+0.0
+2.1
-0.1
-0.3
stress
Spectinomycin 0.00625 mg/ml
+0.8
+0.0
+1.4
+0.0
+0.0
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