Fitness for 5 genes in Escherichia coli BW25113

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500 ntyggX and mltC are separated by 64 nucleotidesmltC and nupG are separated by 201 nucleotidesnupG and speC are separated by 49 nucleotidesspeC and yqgA are separated by 397 nucleotides b2962: yggX - hypothetical protein (NCBI), at 3,102,115 to 3,102,390 yggX b2963: mltC - membrane-bound lytic murein transglycosylase C (VIMSS), at 3,102,455 to 3,103,534 mltC b2964: nupG - transport of nucleosides, permease protein (VIMSS), at 3,103,736 to 3,104,992 nupG b2965: speC - ornithine decarboxylase isozyme (VIMSS), at 3,105,042 to 3,107,177 speC b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282 yqgA
Group Condition b2962 b2963 b2964 b2965 b2966
yggX mltC nupG speC yqgA
carbon source Ying_Nucleobase14 rep C; time point 3 +0.3 +0.1 -3.6 -0.3 -0.1
carbon source Ying_Nucleobase14 rep B; time point 3 +0.2 +0.1 -3.6 -0.2 +0.2
carbon source Ying_Nucleobase14 rep A; time point 3 +0.6 +0.1 -3.5 -0.4 -0.1
phage T7_phage 0.00015625 MOI TC3 -2.3 -0.2 -0.4 -0.2 +0.4
carbon source Ying_Nucleobase14 rep A; time point 2 +0.5 +0.1 -2.9 -0.1 -0.0
phage T7_phage 0.00015625 MOI TC4 -1.7 +0.0 -0.6 +0.2 -0.2
motility outer cut, LB soft agar motility assay -1.3 -0.1 -0.1 -0.3 -0.3
carbon source Ying_Nucleobase14 rep B; time point 2 +0.3 +0.2 -2.5 +0.0 +0.0
carbon source Ying_Nucleobase14 rep C; time point 2 +0.5 +0.1 -2.4 -0.0 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 +0.3 -1.8 +0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 +0.1 -0.5 +0.2 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.2 -1.3 +0.1 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.8 -0.2 -1.9 +0.3 -0.3
protonated/deuterated deuterated glucose in H2O -1.6 -0.0 +0.3 -0.0 +0.2
stress Cephalothin 0.01 mg/ml -1.5 +0.9 +0.2 -0.1 -0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 -1.0 -0.5 -0.1 +0.8
stress octanol 0.08 vol% -0.8 +0.7 +0.1 +0.3 -1.1
carbon source Ying_Nucleobase14 rep B; time point 1 +0.5 -0.0 -1.2 +0.1 -0.2
carbon source Guanosine 0.35306 mM (C) +0.4 +0.1 -1.2 +0.3 -0.3
carbon source Uridine 0.4095 mM (C) +0.2 -0.0 -1.4 +0.8 -0.2
gfp_control GFP_control -0.6 +0.2 +0.6 -0.1 -0.7
carbon source Uridine 0.4095 mM (C) +0.2 -0.0 -1.1 +0.7 -0.1
stress Trimethoprim 0.00125 mM +0.9 +0.1 -0.7 -0.1 -0.2
carbon source Uridine 0.4095 mM (C) -0.0 +0.1 -1.0 +0.9 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.8 -0.7 +0.1 -0.2 +0.4
stress 3-methyl-1-butanol 1.25 vol% +1.0 -0.3 +0.2 +0.3 +0.6
nophagecontrol R1_N4_T1_phage0 +0.6 +0.2 -0.1 +0.2 +1.0
stress 2-Furfuraldehyde 0.25 vol% +2.0 -0.1 -0.2 -0.1 +0.4
stress Spectinomycin 0.0125 mg/ml +0.3 +0.0 +2.1 -0.1 -0.3
stress Spectinomycin 0.00625 mg/ml +0.8 +0.0 +1.4 +0.0 +0.0
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